Detailed information NTG1148853

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C6H37_RS13910
• Genome accession: NZ_LT963408
• Coordinates: 2819592..2820761 (+)
• Length: 389 a.a.
• NCBI ID: WP_005737916.1
• Uniprot ID: A0A2S4I8U6
• Organism: Pseudomonas syringae group genomosp. 3 isolate CFBP6411
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2814592..2825761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H37_RS13895 (CFBP6411_02557)	-	2814955..2817288 (+)	2334	WP_104698447.1	ATP-dependent DNA helicase	-
C6H37_RS13900 (CFBP6411_02558)	pgm	2817389..2819035 (+)	1647	WP_005737913.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
C6H37_RS13905 (CFBP6411_02559)	-	2819162..2819437 (-)	276	WP_005737914.1	peptidylprolyl isomerase	-
C6H37_RS13910 (CFBP6411_02560)	pilU	2819592..2820761 (+)	1170	WP_005737916.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C6H37_RS13920 (CFBP6411_02562)	-	2821508..2823085 (-)	1578	WP_060402672.1	ABC transporter ATP-binding protein	-
C6H37_RS13925 (CFBP6411_02563)	-	2823087..2824109 (-)	1023	WP_005618488.1	ABC transporter permease	-
C6H37_RS13930 (CFBP6411_02564)	-	2824109..2825173 (-)	1065	WP_005737920.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42695.89 Da
• Isoelectric Point: 5.8676

>NTDB_id=1148853 C6H37_RS13910 WP_005737916.1 2819592..2820761(+) (pilU) [Pseudomonas syringae group genomosp. 3 isolate CFBP6411]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGIETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1148853 C6H37_RS13910 WP_005737916.1 2819592..2820761(+) (pilU) [Pseudomonas syringae group genomosp. 3 isolate CFBP6411]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAATTCAACGGCGTGCTCAAACCGCTGGGCATCGAAACGCTCAAGCCCGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAACAGAAGCTCGAGTTTCTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCTGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTTGATATTCCGCGCTTTGA
GGACCTGTTCCTGCCGCCGGTATTGCTCGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGTAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCGCTGAAAAACACCCTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAAACCATGGAGCACG
CCCTGGCATTCGCCGACACCGGCCACTTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGTGCGCAGTTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGCAACGAACTGACCGAACTCAAGGGCATCATGGAAAAGTCTGGCAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCGGTCGAAGGTGCGATCAGCGAGGAAGAAGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAACTGCACAGCGAAAATGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACGGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-248)

3D structure

• AlphaFold DB: A0A2S4I8U6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365