Detailed information NTG1148815

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C6H38_RS14710
• Genome accession: NZ_LT963402
• Coordinates: 3058614..3059783 (-)
• Length: 389 a.a.
• NCBI ID: WP_044389985.1
• Uniprot ID: A0A3M3YY41
• Organism: Pseudomonas syringae pv. avii isolate CFBP3846
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3053614..3064783

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H38_RS14695 (CFBP3846_02805)	-	3054771..3055835 (+)	1065	WP_011104338.1	microcin C ABC transporter permease YejB	-
C6H38_RS14700 (CFBP3846_02806)	-	3055835..3056857 (+)	1023	WP_060412619.1	ABC transporter permease	-
C6H38_RS14705 (CFBP3846_02807)	-	3056859..3058436 (+)	1578	WP_060412620.1	ABC transporter ATP-binding protein	-
C6H38_RS14710 (CFBP3846_02808)	pilU	3058614..3059783 (-)	1170	WP_044389985.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C6H38_RS14715 (CFBP3846_02809)	-	3059938..3060213 (+)	276	WP_003373489.1	peptidylprolyl isomerase	-
C6H38_RS14720 (CFBP3846_02810)	pgm	3060289..3061935 (-)	1647	WP_005764448.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
C6H38_RS14725 (CFBP3846_02811)	-	3062036..3064321 (-)	2286	WP_060412621.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42668.78 Da
• Isoelectric Point: 5.8663

>NTDB_id=1148815 C6H38_RS14710 WP_044389985.1 3058614..3059783(-) (pilU) [Pseudomonas syringae pv. avii isolate CFBP3846]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLSSETLKPGEVAVVAQGLMDEEQKLEFVRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVDGAISEEEALKNADSQNNVRLRLKLHSENGVANLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1148815 C6H38_RS14710 WP_044389985.1 3058614..3059783(-) (pilU) [Pseudomonas syringae pv. avii isolate CFBP3846]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCTGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAGTTCAACGGCGTGCTCAAGCCGCTGAGCAGCGAAACCCTCAAGCCTGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAGTTTGTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCTATTGTCGCGCGTAACATCAAGCTGGACATTCCGCGCTTTGA
GGACCTTTTCCTGCCGCCGGTATTGCTTGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGTGCCACCGGCT
CGGGTAAATCGACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAGGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGTGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACTCTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATCCGCGATCGCGAAACGATGGAGCACG
CGCTGGCATTTGCCGACACCGGCCACTTGGCGATTTCGACCCTGCACGCCAATAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTTTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTGGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATCCGCGACCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGCAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGATGGCGCGATCAGCGAGGAAGAAGCATTGAAAAATGCCGATTCGCAAAACAACGTTCGTCTGCG
CCTCAAGCTGCACAGCGAGAACGGAGTGGCTAACCTGACCACGCCGCCCCCGGCGCCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A3M3YY41

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	52.113	91.26	0.476
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365