Detailed information NTG1148757

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C6H44_RS11305
• Genome accession: NZ_LT963391
• Coordinates: 2363920..2365089 (+)
• Length: 389 a.a.
• NCBI ID: WP_054071721.1
• Uniprot ID: A0A9P3AB78
• Organism: Pseudomonas syringae pv. cerasicola isolate CFBP6109
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2358920..2370089

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C6H44_RS11290	-	2359357..2361684 (+)	2328	Protein_2126	ATP-dependent DNA helicase	-
C6H44_RS11295 (CFBP6109_02225)	pgm	2361800..2363446 (+)	1647	WP_057460406.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
C6H44_RS11300 (CFBP6109_02226)	-	2363521..2363796 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
C6H44_RS11305 (CFBP6109_02227)	pilU	2363920..2365089 (+)	1170	WP_054071721.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
C6H44_RS11310 (CFBP6109_02228)	-	2365140..2366717 (-)	1578	WP_057460405.1	ABC transporter ATP-binding protein	-
C6H44_RS11315 (CFBP6109_02229)	-	2366719..2367741 (-)	1023	WP_060411205.1	ABC transporter permease	-
C6H44_RS11320 (CFBP6109_02230)	-	2367741..2368805 (-)	1065	WP_002553338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42578.79 Da
• Isoelectric Point: 6.0641

>NTDB_id=1148757 C6H44_RS11305 WP_054071721.1 2363920..2365089(+) (pilU) [Pseudomonas syringae pv. cerasicola isolate CFBP6109]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPIGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=1148757 C6H44_RS11305 WP_054071721.1 2363920..2365089(+) (pilU) [Pseudomonas syringae pv. cerasicola isolate CFBP6109]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAACCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCTGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGCT
CGGGCAAATCGACCTCGTTGGCAGCGCTGATCGATTACCGCAACCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTCATCCATCGCCACAAGAAGTCCATCGTCAACCAGCGCGAGGTGGGGGTCGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTGTCCCAGCG
ACTGGTCAGAACCCCGGACGGCAAACGCCGCGCAGCGGTGGAAGTGATGATGGGTACGCCGACCATTCGCGACCTGATCC
AGCGCAACGAACTGACCGAACTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCACTG
TTCAATCTGGCAGTCGAAGGGGCGATCAGCGAGGAGGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGATCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362