Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CKV94_RS03665 Genome accession   NZ_LT906482
Coordinates   728301..729977 (+) Length   558 a.a.
NCBI ID   WP_003823522.1    Uniprot ID   C0DVR4
Organism   Eikenella corrodens strain NCTC10596     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723301..734977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV94_RS03645 (SAMEA4412678_00728) fumC 724326..725720 (-) 1395 WP_035580537.1 class II fumarate hydratase -
  CKV94_RS03660 (SAMEA4412678_00731) recJ 726358..728055 (+) 1698 WP_003823521.1 single-stranded-DNA-specific exonuclease RecJ -
  CKV94_RS03665 (SAMEA4412678_00732) pilF 728301..729977 (+) 1677 WP_003823522.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKV94_RS03670 (SAMEA4412678_00733) pilG 730009..731244 (+) 1236 WP_003823524.1 type II secretion system F family protein Machinery gene
  CKV94_RS03675 (SAMEA4412678_00734) pilD 731319..732176 (+) 858 WP_003823525.1 A24 family peptidase Machinery gene
  CKV94_RS03680 (SAMEA4412678_00735) coaE 732178..732783 (+) 606 WP_003823526.1 dephospho-CoA kinase -
  CKV94_RS03685 (SAMEA4412678_00736) zapD 732787..733563 (+) 777 WP_003823527.1 cell division protein ZapD -
  CKV94_RS03690 (SAMEA4412678_00737) yacG 733560..733742 (+) 183 WP_003823528.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62167.82 Da        Isoelectric Point: 5.1922

>NTDB_id=1148098 CKV94_RS03665 WP_003823522.1 728301..729977(+) (pilF) [Eikenella corrodens strain NCTC10596]
MSVGLLRILFQNKLIEQDQVEKYREKVNQNKNIIPMLAEAGIIKQADLALLLAKLFHYPILDLSLYSRSMMVQDVLNDSQ
MTEYRCVPLFKRGQKLFLGVSDPTLLQTYQKLVFPSGLIVDLILVNDEQLDRLLEFASQTSTAILDEISADSTTLATPAS
LKVDDEEEDGPIAKFIHKVLSDALNTGASDIHFEFYEEMARIRFRTDGQLREVVQPPLNIRGQIASRIKVMSKMDISEKR
VPQDGRIQIQFNKNQKPIDFRVNTLPTLFGEKIVMRILNSDAATLNINQLGFEPFQKELLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGMTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKQPIERPPAEALLKVGFTEEDLAKDDWQLY
RQVGCDRCKGKGFKGRAGVYEIMPVTDAIQKIIINNGTEVDIAEQAYADGLVDLRRAGILKAMQGITTLEEILASTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1148098 CKV94_RS03665 WP_003823522.1 728301..729977(+) (pilF) [Eikenella corrodens strain NCTC10596]
ATGAGCGTCGGACTATTACGCATCCTGTTTCAAAACAAACTGATTGAGCAGGATCAGGTTGAAAAATACCGCGAAAAAGT
TAATCAAAACAAAAATATCATTCCCATGCTAGCTGAGGCTGGCATTATCAAGCAGGCAGATTTGGCACTGCTTCTAGCCA
AGCTGTTCCACTATCCGATTTTGGATTTGTCCCTCTACTCCCGCAGCATGATGGTGCAAGATGTACTCAACGACAGCCAG
ATGACGGAGTACCGCTGCGTACCTTTGTTCAAGCGTGGGCAAAAACTGTTTTTGGGTGTCTCCGACCCCACTTTGTTGCA
AACATACCAAAAGCTTGTTTTCCCTAGCGGCTTGATTGTTGACCTGATCCTAGTCAATGATGAACAGCTAGACCGCCTGT
TGGAGTTCGCCAGCCAAACATCCACCGCCATTTTGGATGAAATTTCTGCTGACTCAACCACCCTGGCCACACCAGCTTCG
CTCAAAGTGGATGACGAAGAAGAAGACGGCCCGATTGCTAAATTCATCCACAAGGTGCTGTCTGATGCCCTTAACACCGG
CGCATCCGATATCCACTTCGAATTCTACGAAGAAATGGCGCGTATCCGCTTCCGTACCGATGGCCAGCTGCGCGAAGTGG
TACAGCCTCCGCTAAATATCCGCGGACAGATTGCCTCACGTATCAAAGTAATGTCCAAAATGGATATTTCCGAAAAACGC
GTCCCACAAGACGGCCGAATCCAGATTCAGTTCAACAAAAACCAAAAGCCCATCGACTTCCGTGTGAATACGCTGCCCAC
GCTATTCGGCGAAAAAATTGTAATGCGTATTTTGAACTCGGATGCCGCCACGCTGAATATCAACCAGCTTGGTTTCGAAC
CTTTCCAAAAAGAATTGCTGCTGGAAGCCATCCACCGCCCGTACGGCATGGTGCTGGTAACTGGTCCGACCGGTTCGGGT
AAAACTGTGTCGCTGTACACCTGCCTAAACATTTTGAATACCGATAGCGTAAACATCTCCACCGCCGAAGACCCGGCCGA
GATTAACTTGCCCGGTATCAATCAGGTAAACGTAAACGACAAACAGGGCATGACATTTGCTGCGGCACTGAAAGCATTCC
TGCGCCAAGACCCGGACATCATCATGGTGGGTGAAATTCGCGACTTGGAAACTGCCGATATCGCCATTAAAGCTGCGCAA
ACCGGTCACATGGTATTCTCTACCCTGCACACCAATAACGCTCCAGCCACACTATCCCGTATGCTGAACATGGGGGTAGC
ACCGTTCAATATTGCCAGCTCGGTTAATCTGATTATGGCTCAGCGTTTGGTGCGTCGACTGTGTACCTGCAAACAACCGA
TCGAGCGTCCGCCAGCCGAGGCTTTACTGAAAGTTGGCTTCACCGAGGAAGACTTGGCCAAGGACGACTGGCAGCTATAC
CGTCAGGTGGGTTGCGACCGTTGCAAAGGCAAAGGTTTCAAAGGCCGAGCCGGTGTATATGAAATCATGCCGGTTACCGA
TGCCATACAAAAAATCATTATCAATAACGGCACCGAGGTGGATATTGCCGAACAGGCCTATGCCGACGGGCTGGTAGACT
TACGCCGAGCTGGTATTTTGAAAGCAATGCAAGGCATTACTACCTTAGAAGAAATCCTTGCCAGCACCAACGACTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

70

100

0.703
  pilB Acinetobacter baylyi ADP1

51.792

100

0.518
  pilB Acinetobacter baumannii D1279779

51.254

100

0.513
  pilB Vibrio cholerae strain A1552

46.479

100

0.473
  pilB Legionella pneumophila strain ERS1305867

46.263

100

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

44.326

100

0.448
  pilB Vibrio campbellii strain DS40M4

43.563

100

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.381

94.086

0.38
  pilF Thermus thermophilus HB27

38.068

94.624

0.36

Multiple sequence alignment