Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKV96_RS07560 Genome accession   NZ_LT906476
Coordinates   1644516..1646240 (+) Length   574 a.a.
NCBI ID   WP_014843847.1    Uniprot ID   -
Organism   Legionella pneumophila strain NCTC11286     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1639516..1651240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV96_RS07540 (SAMEA4040027_01487) - 1639863..1642391 (+) 2529 WP_014843844.1 EAL domain-containing protein -
  CKV96_RS07545 (SAMEA4040027_01488) - 1642393..1642962 (-) 570 WP_014843845.1 hypoxanthine-guanine phosphoribosyltransferase -
  CKV96_RS07550 (SAMEA4040027_01489) - 1643005..1643331 (-) 327 WP_011213802.1 DUF1820 family protein -
  CKV96_RS07555 (SAMEA4040027_01490) - 1643514..1644257 (-) 744 WP_014843846.1 methyltransferase domain-containing protein -
  CKV96_RS07560 (SAMEA4040027_01491) pilB 1644516..1646240 (+) 1725 WP_014843847.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKV96_RS07565 (SAMEA4040027_01492) pilC 1646246..1647466 (+) 1221 WP_027225016.1 type II secretion system F family protein Machinery gene
  CKV96_RS07570 (SAMEA4040027_01493) pilD 1647512..1648375 (+) 864 WP_027225017.1 prepilin peptidase Machinery gene
  CKV96_RS07575 (SAMEA4040027_01494) - 1648389..1649594 (-) 1206 WP_014843849.1 cyclic nucleotide-binding domain-containing protein -
  CKV96_RS07580 (SAMEA4040027_01495) - 1649702..1650295 (-) 594 WP_011213808.1 hypothetical protein -
  CKV96_RS07585 (SAMEA4040027_01496) - 1650311..1650766 (-) 456 WP_011213809.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62710.25 Da        Isoelectric Point: 5.5861

>NTDB_id=1147962 CKV96_RS07560 WP_014843847.1 1644516..1646240(+) (pilB) [Legionella pneumophila strain NCTC11286]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKETDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1147962 CKV96_RS07560 WP_014843847.1 1644516..1646240(+) (pilB) [Legionella pneumophila strain NCTC11286]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGATAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATATCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAATTGCATCGATGTGGGCACCATT
CCTGCCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGAGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAAGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCTCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTGGAAGGTTTAGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCGCCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATACCGAATCAGGTATAGGCAAGATGGTATTTTACATGAGGTCGCCACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGCATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAGGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGTACATGTCCAACCTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCGGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGACCCTGATATTATAATG
GTTGGTGAGATACGAGATCTTGAAACAGCAGAGATTGCTGTAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCTGCTGAAACTTTAAATCGTTTAGTAAATATGGGAATACCTACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGAGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAACTGATCTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTTGGGTTGTTTGAGGTTTTACCGATGACGAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGCTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

99.477

100

0.995
  pilB Acinetobacter baylyi ADP1

49.656

100

0.503
  pilB Acinetobacter baumannii D1279779

50.617

98.78

0.5
  pilB Vibrio cholerae strain A1552

50.926

94.077

0.479
  pilB Vibrio campbellii strain DS40M4

47.756

97.038

0.463
  pilF Neisseria gonorrhoeae MS11

46.996

98.606

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.98

93.902

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

92.16

0.368
  pilF Thermus thermophilus HB27

38.476

93.728

0.361

Multiple sequence alignment