Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   CKV74_RS03500 Genome accession   NZ_LT906463
Coordinates   684073..685467 (+) Length   464 a.a.
NCBI ID   WP_408607456.1    Uniprot ID   -
Organism   Haemophilus pittmaniae strain NCTC13334     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 679073..690467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV74_RS03475 (NCTC13334_00701) - 679156..681774 (-) 2619 WP_095176747.1 penicillin-binding protein 1A -
  CKV74_RS03480 (NCTC13334_00702) pilM 681874..682629 (+) 756 WP_007242451.1 pilus assembly protein PilM -
  CKV74_RS03485 (NCTC13334_00703) - 682638..683150 (+) 513 WP_007242358.1 hypothetical protein -
  CKV74_RS03490 (NCTC13334_00704) - 683147..683674 (+) 528 WP_007242489.1 hypothetical protein -
  CKV74_RS03495 (NCTC13334_00705) - 683671..684036 (+) 366 WP_007242378.1 hypothetical protein -
  CKV74_RS03500 (NCTC13334_00706) comE 684073..685467 (+) 1395 WP_408607456.1 type IV pilus secretin PilQ Machinery gene
  CKV74_RS03505 (NCTC13334_00707) - 685540..686235 (+) 696 WP_007242316.1 ComF family protein -
  CKV74_RS03510 (NCTC13334_00708) nfuA 686313..686897 (+) 585 WP_007242387.1 Fe-S biogenesis protein NfuA -
  CKV74_RS03515 (NCTC13334_00709) hypF 687121..689409 (-) 2289 WP_095176749.1 carbamoyltransferase HypF -
  CKV74_RS03520 (NCTC13334_00710) - 689747..690346 (+) 600 WP_007242385.1 4Fe-4S dicluster domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 50392.51 Da        Isoelectric Point: 7.0302

>NTDB_id=1147718 CKV74_RS03500 WP_408607456.1 684073..685467(+) (comE) [Haemophilus pittmaniae strain NCTC13334]
MARAHRLGKCIYRALLGGLVLLCSTQLAADENSVFSVRLKQAPLVATLQQLALTQNINLLIDDELEGNLSLQLDNVDVDR
LLRATAKIKGLSFHQENGIYYLGKLSENEKYAGQMSTPNTLEPADIAGTSVAVEPNLQHATIKLHFAKAQDVLKTLTGGS
GSLLSANGAVSFDERSNLLLIQDDARSLRNIKKLVAELDKPIEQIAIEARIVTITDESLKELGVRWGIFNPAAESHRVSG
SLAANGFNDIANQLNINFAGAINPAGSVALQVAKINGRLLDLELTALERENNVEIIASPRLLTTNKKSASIKQGTEIPYV
VTNGRNDTQSVEFREAVLGLEVTPHISKDNTILLDLLVSQNSPGNRVSYGQNSEVVSIDKQEINTQVFAKNGETIVLGGV
FHDTISKGEDKVPLLGDIPGIKRLFSRQSERHQKRELVIFVTPHILTPGPLPAGFHPGKQKKSQ

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1147718 CKV74_RS03500 WP_408607456.1 684073..685467(+) (comE) [Haemophilus pittmaniae strain NCTC13334]
ATAGCGAGGGCGCATAGGCTAGGAAAATGCATATATCGTGCCTTATTAGGGGGATTAGTGCTGTTATGCAGTACTCAGCT
TGCCGCCGATGAGAATTCGGTGTTTTCGGTTCGTCTTAAACAGGCGCCATTAGTGGCGACATTACAGCAGCTGGCACTAA
CGCAAAATATCAATTTGTTAATTGATGATGAGCTAGAAGGCAATTTATCCCTGCAGTTGGATAATGTGGATGTGGATCGT
CTATTACGTGCAACTGCCAAAATTAAAGGTCTTTCCTTTCATCAAGAAAACGGCATCTACTATCTCGGCAAATTATCGGA
AAATGAAAAATATGCCGGACAAATGAGTACACCCAATACGCTCGAACCTGCGGATATAGCTGGAACTTCTGTAGCGGTGG
AACCCAATTTGCAACATGCCACAATCAAATTACATTTTGCGAAAGCGCAGGATGTTCTGAAAACTCTTACCGGAGGTAGT
GGTAGCCTGCTTTCAGCGAATGGTGCAGTAAGTTTTGATGAACGCAGTAATCTGCTATTAATTCAGGATGATGCGCGTTC
CTTAAGAAATATTAAAAAATTAGTTGCCGAACTGGATAAGCCCATCGAACAAATTGCGATAGAGGCCCGCATTGTTACGA
TTACCGATGAAAGCCTGAAAGAATTGGGAGTGCGCTGGGGGATCTTTAATCCTGCGGCAGAGTCACATCGAGTTTCGGGC
AGTTTGGCTGCCAATGGTTTCAACGATATTGCTAATCAGCTAAATATCAATTTTGCCGGAGCGATTAATCCGGCCGGTTC
CGTTGCGTTGCAGGTGGCGAAAATTAATGGCCGCCTATTGGATTTGGAATTAACCGCTTTAGAGCGTGAAAATAATGTGG
AAATCATCGCTAGTCCACGTTTGCTCACAACTAATAAAAAGAGTGCCAGTATCAAGCAAGGAACCGAAATTCCTTATGTG
GTTACTAATGGGCGTAATGATACGCAGTCGGTAGAGTTTCGCGAAGCTGTACTTGGGCTTGAAGTGACACCTCATATATC
CAAGGATAATACAATTTTGTTGGATTTATTGGTTAGTCAAAACTCACCAGGGAATCGGGTTTCTTACGGACAAAATAGTG
AAGTTGTCTCAATTGATAAACAGGAAATTAATACGCAAGTGTTTGCTAAAAATGGTGAAACTATTGTGCTGGGCGGAGTA
TTTCATGACACCATCAGTAAGGGCGAAGACAAGGTGCCGTTGTTGGGAGATATTCCTGGGATAAAACGATTGTTTAGCCG
ACAAAGCGAACGTCATCAAAAAAGGGAATTGGTGATTTTTGTCACTCCGCATATTCTCACTCCAGGCCCCTTGCCGGCAG
GATTTCATCCTGGCAAGCAAAAGAAATCGCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(286-446)

Bacterial type II/III secretion system short domain (Secretin_N)

(141-204)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

68.934

95.043

0.655
  comE Haemophilus influenzae 86-028NP

68.481

95.043

0.651
  comE Glaesserella parasuis strain SC1401

50.467

92.241

0.466
  pilQ Vibrio campbellii strain DS40M4

41.096

94.397

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.299

92.888

0.384
  pilQ Vibrio cholerae strain A1552

41.299

92.888

0.384

Multiple sequence alignment