Detailed information NTG1147573

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CKW05_RS09855
• Genome accession: NZ_LT906457
• Coordinates: 2194315..2195379 (-)
• Length: 354 a.a.
• NCBI ID: WP_058482985.1
• Uniprot ID: A0A0W0Z6Y4
• Organism: Legionella spiritensis strain NCTC11990
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2189315..2200379

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKW05_RS09830 (SAMEA4412668_01965)	aroK	2189828..2190355 (-)	528	WP_058482981.1	shikimate kinase AroK	-
CKW05_RS09835 (SAMEA4412668_01966)	pilQ	2190443..2192584 (-)	2142	WP_058482982.1	type IV pilus secretin PilQ	Machinery gene
CKW05_RS09840 (SAMEA4412668_01967)	pilP	2192594..2193100 (-)	507	WP_058483009.1	pilus assembly protein PilP	Machinery gene
CKW05_RS09845 (SAMEA4412668_01968)	pilO	2193115..2193735 (-)	621	WP_058482983.1	type 4a pilus biogenesis protein PilO	Machinery gene
CKW05_RS09850 (SAMEA4412668_01969)	pilN	2193732..2194310 (-)	579	WP_058482984.1	PilN domain-containing protein	Machinery gene
CKW05_RS09855 (SAMEA4412668_01970)	pilM	2194315..2195379 (-)	1065	WP_058482985.1	pilus assembly protein PilM	Machinery gene
CKW05_RS09860 (SAMEA4412668_01971)	-	2195724..2196842 (+)	1119	WP_058482986.1	hypothetical protein	-
CKW05_RS09865 (SAMEA4412668_01973)	-	2197026..2199392 (+)	2367	WP_058482987.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38515.32 Da
• Isoelectric Point: 4.6040

>NTDB_id=1147573 CKW05_RS09855 WP_058482985.1 2194315..2195379(-) (pilM) [Legionella spiritensis strain NCTC11990]
MLKLFKPKNRSVIGIDISSSSVKALEISTNDNTYCIEGYGYENLPANALDGNTIKDIDAVASCIKRVLVNAHLHGKSVVL
AVPDSSVISKVVQINEGLSDSEIEELIVIEADKYIPYPIDEINIDFEVLGHSPKNSAMLDVLIVASRAENVSSRVEAVSR
AGLEAKIVDVESFAVERAAQLLAKDLPAQGQDKIVAVIDIGSSFTDLFVLHGMKIIFTREEEFGGKQLIEAIARQYSVSF
DEAAAMKAAGRMPEDYEDAVLQPYIEMILLQIKRTLQFFFSTSHHGFVDHIILAGGVAMLPNLADLVQEKTGIPTSVANP
FDKMEIAPQLDRGRMLNEGPSLMIACGLALRTIG

Nucleotide

• Length: 1065 bp

>NTDB_id=1147573 CKW05_RS09855 WP_058482985.1 2194315..2195379(-) (pilM) [Legionella spiritensis strain NCTC11990]
GTGCTCAAACTGTTTAAACCTAAAAATCGATCAGTTATCGGTATTGATATCAGTTCCTCTTCCGTCAAGGCTCTGGAAAT
TTCCACTAATGACAATACCTATTGTATTGAAGGCTATGGTTATGAAAACCTTCCCGCCAATGCTCTGGACGGCAACACCA
TCAAGGATATTGACGCCGTCGCCTCCTGTATCAAACGGGTTCTGGTTAATGCTCATTTGCATGGCAAATCCGTGGTTCTC
GCGGTTCCTGATTCCTCAGTCATCAGCAAGGTCGTACAGATTAACGAAGGCTTGAGCGATTCGGAAATCGAGGAGCTTAT
CGTTATCGAAGCCGATAAATACATCCCTTATCCGATTGATGAAATCAATATAGACTTTGAGGTTCTGGGACATTCACCAA
AAAATTCCGCCATGCTCGATGTGTTGATTGTCGCATCGCGCGCTGAAAATGTAAGCAGTCGGGTTGAAGCCGTCAGCCGT
GCCGGTCTGGAAGCGAAAATAGTCGATGTCGAATCCTTTGCCGTAGAGCGGGCCGCTCAATTGCTTGCTAAAGACTTGCC
GGCCCAGGGGCAGGATAAAATTGTCGCGGTTATCGATATTGGCTCCTCGTTTACCGACTTGTTTGTCTTGCACGGGATGA
AAATCATCTTTACCCGGGAAGAGGAGTTTGGCGGCAAACAGTTGATAGAGGCTATTGCCAGACAATACTCGGTCAGTTTT
GATGAGGCGGCGGCAATGAAAGCGGCAGGCCGAATGCCGGAAGATTATGAAGATGCGGTGTTGCAACCCTATATAGAAAT
GATTCTTCTGCAAATCAAACGTACTCTGCAATTTTTCTTTTCAACCAGTCATCACGGGTTTGTCGATCATATTATTCTGG
CCGGCGGGGTGGCCATGCTGCCGAATTTAGCCGATCTGGTGCAGGAAAAAACAGGAATTCCGACCAGCGTGGCCAATCCC
TTCGATAAAATGGAAATAGCCCCTCAACTGGACAGGGGGCGTATGTTGAATGAAGGTCCCTCCCTGATGATCGCCTGCGG
ATTGGCTCTAAGGACGATTGGATGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: A0A0W0Z6Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	64.589	99.718	0.644
comM	Acinetobacter nosocomialis M2	43.059	99.718	0.429
pilM	Acinetobacter baumannii D1279779	43.059	99.718	0.429
comM	Acinetobacter baylyi ADP1	42.21	99.718	0.421