Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   CKW05_RS09850 Genome accession   NZ_LT906457
Coordinates   2193732..2194310 (-) Length   192 a.a.
NCBI ID   WP_058482984.1    Uniprot ID   -
Organism   Legionella spiritensis strain NCTC11990     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2188732..2199310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW05_RS09825 (SAMEA4412668_01964) aroB 2188735..2189841 (-) 1107 WP_058482980.1 3-dehydroquinate synthase -
  CKW05_RS09830 (SAMEA4412668_01965) aroK 2189828..2190355 (-) 528 WP_058482981.1 shikimate kinase AroK -
  CKW05_RS09835 (SAMEA4412668_01966) pilQ 2190443..2192584 (-) 2142 WP_058482982.1 type IV pilus secretin PilQ Machinery gene
  CKW05_RS09840 (SAMEA4412668_01967) pilP 2192594..2193100 (-) 507 WP_058483009.1 pilus assembly protein PilP Machinery gene
  CKW05_RS09845 (SAMEA4412668_01968) pilO 2193115..2193735 (-) 621 WP_058482983.1 type 4a pilus biogenesis protein PilO Machinery gene
  CKW05_RS09850 (SAMEA4412668_01969) pilN 2193732..2194310 (-) 579 WP_058482984.1 PilN domain-containing protein Machinery gene
  CKW05_RS09855 (SAMEA4412668_01970) pilM 2194315..2195379 (-) 1065 WP_058482985.1 pilus assembly protein PilM Machinery gene
  CKW05_RS09860 (SAMEA4412668_01971) - 2195724..2196842 (+) 1119 WP_058482986.1 hypothetical protein -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22231.67 Da        Isoelectric Point: 9.7596

>NTDB_id=1147572 CKW05_RS09850 WP_058482984.1 2193732..2194310(-) (pilN) [Legionella spiritensis strain NCTC11990]
MTEINLLPWRELKREHEKKQFTTMLFGALVLAAGLVFMINYYAISLVDGQTRRNQRLQDEITIFNKQIQEIKKLKEIREA
LISRMNIVQNLQATRTLTVHLFDELVKVVPDGVYLTKVKRVEHRVTVLGYSESNSSVSTLMRNIELNPWINDPELTEIKK
NIDSKTGDYAQTSNEFKLSFTLKSDNKIDGKS

Nucleotide


Download         Length: 579 bp        

>NTDB_id=1147572 CKW05_RS09850 WP_058482984.1 2193732..2194310(-) (pilN) [Legionella spiritensis strain NCTC11990]
ATGACCGAGATAAACCTTTTACCCTGGCGGGAACTTAAGCGGGAACATGAAAAAAAGCAATTCACAACCATGTTGTTTGG
CGCGCTTGTTCTGGCTGCAGGTCTCGTTTTTATGATTAATTATTACGCTATCAGTCTTGTTGATGGACAGACTCGCCGCA
ACCAGCGTTTGCAGGATGAAATTACAATTTTTAACAAACAGATTCAGGAAATAAAAAAACTGAAAGAAATTCGCGAAGCT
CTGATTTCACGAATGAATATCGTACAGAATTTGCAGGCAACCAGAACATTGACCGTTCATCTCTTTGATGAACTGGTGAA
GGTGGTGCCGGATGGAGTGTATCTGACCAAAGTTAAGCGTGTGGAGCACCGTGTTACCGTTCTTGGTTATTCGGAATCCA
ATTCCAGCGTGTCTACGCTGATGCGTAACATTGAACTCAATCCATGGATTAATGATCCGGAATTAACGGAAATCAAGAAA
AACATAGACTCGAAAACAGGGGATTATGCCCAGACCAGTAATGAGTTCAAATTGAGTTTCACATTGAAGTCGGACAACAA
GATAGATGGTAAATCATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

63.388

95.313

0.604

Multiple sequence alignment