Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKV84_RS07315 Genome accession   NZ_LT906452
Coordinates   1587300..1589024 (+) Length   574 a.a.
NCBI ID   WP_010947251.1    Uniprot ID   -
Organism   Legionella pneumophila strain NCTC11985     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1582300..1594024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV84_RS07295 (SAMEA4040028_01442) - 1583376..1585097 (+) 1722 WP_014841647.1 amidase family protein -
  CKV84_RS07300 (SAMEA4040028_01443) - 1585177..1585746 (-) 570 WP_014841648.1 hypoxanthine-guanine phosphoribosyltransferase -
  CKV84_RS07305 (SAMEA4040028_01444) - 1585789..1586115 (-) 327 WP_011213802.1 DUF1820 family protein -
  CKV84_RS07310 (SAMEA4040028_01445) - 1586298..1587041 (-) 744 WP_014841649.1 methyltransferase domain-containing protein -
  CKV84_RS07315 (SAMEA4040028_01446) pilB 1587300..1589024 (+) 1725 WP_010947251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKV84_RS07320 (SAMEA4040028_01447) pilC 1589030..1590250 (+) 1221 WP_014841650.1 type II secretion system F family protein Machinery gene
  CKV84_RS07325 (SAMEA4040028_01448) pilD 1590295..1591158 (+) 864 WP_014841651.1 prepilin peptidase Machinery gene
  CKV84_RS07330 (SAMEA4040028_01449) - 1591172..1592377 (-) 1206 WP_014841652.1 cyclic nucleotide-binding domain-containing protein -
  CKV84_RS07335 (SAMEA4040028_01450) - 1592485..1593078 (-) 594 WP_011213808.1 hypothetical protein -
  CKV84_RS07340 (SAMEA4040028_01451) - 1593094..1593549 (-) 456 WP_014841654.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62728.28 Da        Isoelectric Point: 5.5861

>NTDB_id=1147432 CKV84_RS07315 WP_010947251.1 1587300..1589024(+) (pilB) [Legionella pneumophila strain NCTC11985]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGTAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1147432 CKV84_RS07315 WP_010947251.1 1587300..1589024(+) (pilB) [Legionella pneumophila strain NCTC11985]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGATAAAACAAA
AGCAATTGAACTACACAAGCTGGCAGCAGCAGAAAAGATGTCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCTATGTTGGATATTAATTGCATCGATGTGGGCACTATT
CCTGCCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAAGCTTCATTAAAGGAAATTCAGTTTCACACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCCCTGATTGATAATCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTGGAAGGTTTAGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCACCTATCGTAAAGTTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATACCGAATCAGGTACAGGCAAGATGGTATTTTACATGAAGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCTCAGGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGGGCAATTGATTTCAGAGTGAGTACATGTCCAACTTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTACTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGCGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATCATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTGTAAAAGCTGCTCAGACAGGACATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAACATGGGAATACCAACTTTTAACATTGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGAGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAGCTGATCTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGCGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTCGGGTTGTTTGAGGTTTTACCGATGACGAAAGAGCTTGGTCAATTGATTATGTCAGGTGGTA
ATTCGCTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATTTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

99.303

100

0.993
  pilB Acinetobacter baylyi ADP1

49.656

100

0.503
  pilB Acinetobacter baumannii D1279779

50.617

98.78

0.5
  pilB Vibrio cholerae strain A1552

50.741

94.077

0.477
  pilF Neisseria gonorrhoeae MS11

46.82

98.606

0.462
  pilB Vibrio campbellii strain DS40M4

47.397

97.038

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

48.609

93.902

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

92.16

0.368
  pilF Thermus thermophilus HB27

38.662

93.728

0.362

Multiple sequence alignment