Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKV79_RS07335 Genome accession   NZ_LT906451
Coordinates   1626816..1628522 (+) Length   568 a.a.
NCBI ID   WP_028372968.1    Uniprot ID   A0A0W0VJ03
Organism   Legionella lansingensis strain NCTC12830     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1621816..1633522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV79_RS07305 (SAMEA44548918_01482) - 1622305..1622805 (-) 501 WP_028372974.1 hypothetical protein -
  CKV79_RS07310 (SAMEA44548918_01483) - 1623251..1624504 (-) 1254 WP_028372973.1 MFS transporter -
  CKV79_RS07315 (SAMEA44548918_01484) - 1624574..1625134 (-) 561 WP_028372972.1 hypoxanthine-guanine phosphoribosyltransferase -
  CKV79_RS07320 (SAMEA44548918_01485) - 1625158..1625484 (-) 327 WP_028372971.1 DUF1820 family protein -
  CKV79_RS07325 (SAMEA44548918_01486) - 1625548..1626315 (-) 768 WP_051546124.1 methyltransferase domain-containing protein -
  CKV79_RS07330 (SAMEA44548918_01487) - 1626437..1626685 (-) 249 WP_028372969.1 hypothetical protein -
  CKV79_RS07335 (SAMEA44548918_01488) pilB 1626816..1628522 (+) 1707 WP_028372968.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKV79_RS07340 (SAMEA44548918_01489) pilC 1628524..1629741 (+) 1218 WP_028372967.1 type II secretion system F family protein Machinery gene
  CKV79_RS07345 (SAMEA44548918_01490) - 1629738..1630601 (+) 864 WP_408606902.1 prepilin peptidase -
  CKV79_RS07350 (SAMEA44548918_01491) - 1630706..1631296 (-) 591 WP_131796106.1 triacylglycerol lipase -
  CKV79_RS07355 (SAMEA44548918_01492) - 1631469..1632056 (-) 588 WP_035915296.1 hypothetical protein -
  CKV79_RS07360 (SAMEA44548918_01493) - 1632069..1632524 (-) 456 WP_028372963.1 hypothetical protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63747.63 Da        Isoelectric Point: 6.7379

>NTDB_id=1147400 CKV79_RS07335 WP_028372968.1 1626816..1628522(+) (pilB) [Legionella lansingensis strain NCTC12830]
MEIQEEQFLHPISQLLVQTNFFTEIKMLKYQKEARSTKQTLPQYLIKNKLISSQKLAWLLSLHFGIDFLDLDSVNNDLIP
WKLIDEKIMRQYHLAPLFVRGNQLFLAIDDPSNQIALKEVQFHTGFYPMPVVVESDKLMHFIEKLAQIRLKQGPTNDHPT
LQLQSPDIEDVYDANLEASRDDGPIVKFVNAILSGAIHRKASDIHFEPYEDRYRIRYRQDGILVEVASPSIHLASRVAAR
VKIMSNLDISERRLPQDGRFKMKFSEKEAIDFRVSICPIVNGEKVVIRILDPNTPKIPIEELGLNATQQELFLQAIGKPQ
GMVLVTGPTGSGKTVSLYAAINLLNTQDKNILTAEDPVEIKIPGVNQVHINPKAGLTFATTLRSFLRQDPDIIMIGEIRD
AETAEMAINAAQTGHLVLSTLHTNSAVESLTRLNNMGIPSFNIASSVSLLIAQRLARRLCNDCKVERKDLSSHDLFMLGF
DKESAVNIKLYQRCGCKHCIDGYSGRIGLFEVVPISKTIGDLILAGQGTVDIMKQAQNEGMITLYESGIEKIKAGLTTIE
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1147400 CKV79_RS07335 WP_028372968.1 1626816..1628522(+) (pilB) [Legionella lansingensis strain NCTC12830]
ATGGAAATTCAGGAAGAACAATTTTTACACCCAATTTCTCAGCTATTGGTTCAAACAAATTTTTTTACTGAGATAAAAAT
GCTTAAATATCAAAAGGAAGCTCGCTCTACCAAGCAGACTTTACCTCAGTATTTGATCAAGAATAAGCTAATCTCTTCAC
AAAAATTAGCATGGCTGCTCTCTCTTCATTTTGGAATAGATTTTCTTGATTTGGATAGTGTTAATAATGATCTCATCCCC
TGGAAACTCATTGACGAAAAAATCATGCGTCAATATCACTTGGCACCTCTTTTTGTGCGTGGGAATCAATTATTCCTAGC
TATTGACGATCCTAGTAATCAAATTGCTCTCAAAGAAGTTCAATTTCACACAGGTTTTTATCCGATGCCAGTTGTCGTGG
AAAGTGACAAATTAATGCATTTTATCGAAAAGCTAGCTCAAATAAGGCTCAAACAAGGTCCAACGAATGATCACCCTACT
CTCCAATTACAGAGTCCAGATATCGAAGATGTTTATGATGCAAATCTAGAAGCATCGCGCGATGATGGTCCAATCGTTAA
ATTTGTCAATGCAATTCTTTCAGGTGCAATACACCGGAAAGCCTCTGATATTCATTTCGAACCGTATGAGGATAGATACC
GTATTCGTTATCGTCAGGATGGAATTTTAGTCGAAGTTGCATCACCATCAATCCACCTGGCAAGTAGAGTGGCAGCTCGC
GTAAAAATTATGTCCAATTTGGATATTTCTGAGCGCCGTCTACCTCAAGACGGTCGTTTTAAAATGAAATTTTCTGAAAA
AGAAGCCATTGATTTCCGAGTAAGTATTTGTCCAATTGTTAATGGCGAAAAAGTTGTCATCCGTATCCTTGATCCCAATA
CTCCCAAAATTCCCATCGAGGAACTTGGATTGAATGCTACTCAACAGGAACTCTTCTTACAGGCCATCGGAAAACCTCAG
GGGATGGTTTTAGTGACTGGACCTACCGGTAGTGGTAAGACCGTATCTCTCTATGCAGCGATCAATCTCCTAAATACCCA
GGATAAAAATATCTTAACTGCAGAGGATCCTGTTGAAATCAAGATACCTGGGGTCAATCAGGTACACATCAATCCCAAAG
CGGGACTAACCTTTGCAACAACGCTGCGCTCCTTTTTGCGACAAGATCCTGACATTATCATGATCGGTGAAATCAGAGAC
GCAGAAACAGCTGAGATGGCTATAAATGCAGCCCAAACCGGACATCTTGTGCTTTCTACTTTGCATACAAATAGTGCAGT
TGAGAGCTTAACACGATTAAATAATATGGGCATTCCTTCATTCAATATTGCCAGTTCTGTTAGTTTATTAATTGCTCAGC
GATTAGCAAGACGATTATGTAACGATTGCAAGGTTGAGCGTAAAGACCTTTCTTCACACGATTTATTTATGCTTGGGTTT
GATAAAGAGAGTGCGGTGAATATCAAACTCTATCAACGTTGCGGATGTAAGCACTGCATCGATGGGTACAGCGGGCGCAT
TGGCTTATTTGAAGTGGTACCCATCTCAAAAACAATTGGGGATCTTATTCTCGCGGGACAAGGGACAGTAGATATTATGA
AGCAAGCCCAAAATGAAGGAATGATAACGCTTTATGAGTCTGGCATTGAGAAAATCAAAGCAGGTTTAACAACAATCGAG
GAAGTTAATCGCGTCACGACAAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0VJ03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.544

100

0.577
  pilB Acinetobacter baumannii D1279779

46.892

99.12

0.465
  pilB Acinetobacter baylyi ADP1

46.466

99.648

0.463
  pilF Neisseria gonorrhoeae MS11

45.161

98.239

0.444
  pilB Vibrio cholerae strain A1552

45.926

95.07

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

43.19

98.239

0.424
  pilB Vibrio campbellii strain DS40M4

42.553

99.296

0.423
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.38

100

0.364

Multiple sequence alignment