Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CKW04_RS06195 Genome accession   NZ_LT906442
Coordinates   1314506..1315570 (+) Length   354 a.a.
NCBI ID   WP_058479248.1    Uniprot ID   A0A0W1ANT4
Organism   Legionella waltersii strain NCTC13017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1309506..1320570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW04_RS06190 (SAMEA4504053_01261) - 1311959..1312933 (-) 975 WP_058479247.1 hypothetical protein -
  CKW04_RS06195 (SAMEA4504053_01263) pilM 1314506..1315570 (+) 1065 WP_058479248.1 type IV pilus assembly protein PilM Machinery gene
  CKW04_RS06200 (SAMEA4504053_01264) pilN 1315575..1316123 (+) 549 WP_058479281.1 PilN domain-containing protein Machinery gene
  CKW04_RS06205 (SAMEA4504053_01265) pilO 1316135..1316734 (+) 600 WP_058479249.1 type 4a pilus biogenesis protein PilO Machinery gene
  CKW04_RS06210 (SAMEA4504053_01266) pilP 1316734..1317255 (+) 522 WP_408607318.1 pilus assembly protein PilP Machinery gene
  CKW04_RS06215 (SAMEA4504053_01267) pilQ 1317259..1319352 (+) 2094 WP_058479251.1 type IV pilus secretin PilQ Machinery gene
  CKW04_RS06220 (SAMEA4504053_01268) aroK 1319695..1320222 (+) 528 WP_058479252.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38969.10 Da        Isoelectric Point: 4.8010

>NTDB_id=1147226 CKW04_RS06195 WP_058479248.1 1314506..1315570(+) (pilM) [Legionella waltersii strain NCTC13017]
MLKLLKPKQHSILGIDISSSAVKVLEISGSGEQLMVECYGREELLPGSLEGNVIKDVDAVASCIKKLVDRFKFTTKKVAL
AVPDSAVISKVVQINDGLSDQEIEELVIIEADKYIPYPIDEINLDFEVLGHSPKNAAMLDVLIVASRAENVSGRVDAVTK
AGLEPKVVDVESYAVERAAQQLARELPASGQDKIIAIIDIGANYTHLFVLHGMKIIFSREEKFGVMQLLEAIAEHYKMSI
EQAKEAKNTGNLPTDYETEVLEPFKEMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPDLAHLIQDQLGVTTTVANP
LSYMSFNKMVNMESITNEAPTLMVACGLALRNVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1147226 CKW04_RS06195 WP_058479248.1 1314506..1315570(+) (pilM) [Legionella waltersii strain NCTC13017]
GTGCTCAAGCTTTTAAAACCTAAACAGCATTCAATTCTTGGAATTGATATTAGCTCATCTGCAGTGAAAGTCCTTGAAAT
TTCCGGTTCTGGTGAACAACTCATGGTTGAGTGCTATGGCCGTGAAGAACTTCTACCTGGCTCATTAGAAGGAAACGTCA
TTAAAGACGTCGATGCAGTAGCAAGTTGCATAAAGAAACTAGTTGATAGATTTAAGTTTACTACCAAAAAAGTTGCTTTA
GCCGTGCCAGATTCTGCAGTGATTAGTAAAGTCGTTCAAATCAATGATGGCTTAAGTGATCAAGAGATAGAGGAACTTGT
TATTATTGAAGCAGATAAATACATCCCTTATCCTATAGATGAGATCAACTTGGATTTTGAGGTATTGGGTCACTCACCAA
AAAACGCAGCCATGTTAGATGTTCTTATTGTTGCTTCTCGGGCTGAAAATGTAAGTGGTAGAGTCGATGCAGTCACTAAA
GCTGGTTTAGAGCCCAAAGTGGTTGATGTGGAATCCTATGCAGTAGAGAGAGCCGCCCAGCAATTAGCTCGAGAACTACC
TGCATCTGGTCAGGACAAAATCATTGCAATTATTGATATTGGGGCAAACTACACGCATTTATTTGTCCTTCATGGTATGA
AAATTATATTTTCTAGAGAGGAAAAATTTGGTGTGATGCAATTGTTAGAAGCTATTGCAGAACATTATAAAATGAGTATC
GAACAGGCTAAGGAAGCTAAAAATACAGGGAATTTGCCTACTGATTATGAAACTGAAGTGCTTGAGCCATTTAAAGAAAT
GATATTATTGCAAATAAAGCGGACATTGCAGTTCTTTTACTCAACCAGTCAACATGGATTTGTTGATCATATTTTATTGG
CAGGCGGGATGGCTAGACAGCCTGATTTAGCTCATTTGATACAAGATCAACTTGGAGTTACCACAACCGTAGCGAACCCT
TTGTCGTATATGTCATTTAATAAAATGGTTAACATGGAATCAATAACCAATGAAGCTCCCACTTTAATGGTGGCTTGTGG
TTTGGCTCTAAGGAATGTTGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W1ANT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

80.226

100

0.802
  pilM Acinetobacter baumannii D1279779

43.785

100

0.438
  comM Acinetobacter nosocomialis M2

43.785

100

0.438
  comM Acinetobacter baylyi ADP1

42.373

100

0.424

Multiple sequence alignment