Detailed information NTG0114635

Bioinformatically predicted

Overview

• Name: pknB
• Type: Regulator
• Locus tag: QI18_RS10250
• Genome accession: NZ_CP010050
• Coordinates: 2079289..2081172 (-)
• Length: 627 a.a.
• NCBI ID: WP_039116083.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk
• Function: modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology) Competence regulation

Genomic Context

Location: 2074289..2086172

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QI18_RS10220 (QI18_10220)	-	2074695..2075375 (-)	681	WP_012898438.1	5'-methylthioadenosine/adenosylhomocysteine nucleosidase	-
QI18_RS10225 (QI18_10225)	macP	2075408..2075686 (-)	279	WP_003130796.1	cell wall synthase accessory phosphoprotein MacP	-
QI18_RS10230 (QI18_10230)	-	2075679..2076263 (-)	585	WP_012898439.1	NUDIX hydrolase	-
QI18_RS10235 (QI18_10235)	glmU	2076368..2077744 (-)	1377	WP_003130798.1	bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU	-
QI18_RS10240 (QI18_10240)	proC	2077972..2078760 (+)	789	WP_012898440.1	pyrroline-5-carboxylate reductase	-
QI18_RS10245 (QI18_10245)	rpsO	2078838..2079107 (-)	270	WP_010906184.1	30S ribosomal protein S15	-
QI18_RS10250 (QI18_10250)	pknB	2079289..2081172 (-)	1884	WP_039116083.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
QI18_RS10255 (QI18_10255)	-	2081172..2081948 (-)	777	WP_039116084.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
QI18_RS10260 (QI18_10260)	rsmB	2082029..2083303 (-)	1275	WP_014570715.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
QI18_RS10270 (QI18_10270)	-	2084506..2085360 (-)	855	WP_039116086.1	GH25 family lysozyme	-
QI18_RS10275 (QI18_10275)	-	2085362..2085814 (-)	453	WP_039116087.1	phage holin	-

Sequence

Protein

• Length: 627 a.a.
• Molecular weight: 67929.01 Da
• Isoelectric Point: 4.7019

>NTDB_id=114635 QI18_RS10250 WP_039116083.1 2079289..2081172(-) (pknB) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKEHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVVNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLDQAKSKIKDAKLKVGTVHKQQSSTI
AEGNVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPTVGA
SVDPTQEIVITYSVYTAPSSSSTTSESTTTPETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide

• Length: 1884 bp

>NTDB_id=114635 QI18_RS10250 WP_039116083.1 2079289..2081172(-) (pknB) [Lactococcus lactis subsp. lactis strain S0 isolate fresh raw milk]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAACTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATCAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGATCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGAACATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGTAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CGCTTGACCAAGCAAAAAGCAAAATTAAAGATGCAAAACTAAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAATGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATTTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCCGACGGTTATGTATATTCTGTTTATCCGACTGTTGGAGCG
TCCGTTGATCCAACTCAAGAAATTGTAATCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACAACGACGCCTGAAACTTCGTCAAGCACAACTTCAAGTACTTCAAGTTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

• Protein kinase domain (Pkinase) (12-265)

• PASTA domain (PASTA) (506-573)

• PASTA domain (PASTA) (365-428)

• PASTA domain (PASTA) (434-501)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pknB	Streptococcus mutans UA159	48.897	86.762	0.424
stkP/pknB	Streptococcus salivarius strain HSISS4	51.06	82.775	0.423
stkP	Streptococcus pneumoniae TIGR4	48.024	80.702	0.388
stkP	Streptococcus pneumoniae D39	47.826	80.702	0.386
stkP	Streptococcus pneumoniae R6	47.826	80.702	0.386
stkP	Streptococcus pneumoniae Rx1	47.628	80.702	0.384