Detailed information NTG1145564

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: H044120014_RS04700
• Genome accession: NZ_LT632616
• Coordinates: 1008643..1009707 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain ST37 isolate ST37
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1003643..1014707

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H044120014_RS04690 (BT148_RS04735)	-	1004680..1007064 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
H044120014_RS04695 (BT148_RS04740)	ravI	1007216..1008226 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
H044120014_RS04700 (BT148_RS04745)	pilM	1008643..1009707 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
H044120014_RS04705 (BT148_RS04750)	pilN	1009711..1010259 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
H044120014_RS04710 (BT148_RS04755)	pilO	1010273..1010872 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
H044120014_RS04715 (BT148_RS04760)	pilP	1010869..1011456 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
H044120014_RS04720 (BT148_RS04765)	pilQ	1011460..1013556 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
H044120014_RS04725 (BT148_RS04770)	aroK	1014002..1014529 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=1145564 H044120014_RS04700 WP_010946662.1 1008643..1009707(+) (pilM) [Legionella pneumophila strain ST37 isolate ST37]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1145564 H044120014_RS04700 WP_010946662.1 1008643..1009707(+) (pilM) [Legionella pneumophila strain ST37 isolate ST37]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418