Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H044120014_RS04710 Genome accession   NZ_LT632616
Coordinates   1010273..1010872 (+) Length   199 a.a.
NCBI ID   WP_014841277.1    Uniprot ID   A0AAV2UVV9
Organism   Legionella pneumophila strain ST37 isolate ST37     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1005273..1015872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H044120014_RS04695 (BT148_RS04740) ravI 1007216..1008226 (-) 1011 WP_010946661.1 Dot/Icm T4SS effector RavI -
  H044120014_RS04700 (BT148_RS04745) pilM 1008643..1009707 (+) 1065 WP_010946662.1 type IV pilus assembly protein PilM Machinery gene
  H044120014_RS04705 (BT148_RS04750) pilN 1009711..1010259 (+) 549 WP_010946663.1 PilN domain-containing protein Machinery gene
  H044120014_RS04710 (BT148_RS04755) pilO 1010273..1010872 (+) 600 WP_014841277.1 type 4a pilus biogenesis protein PilO Machinery gene
  H044120014_RS04715 (BT148_RS04760) pilP 1010869..1011456 (+) 588 WP_010946665.1 pilus assembly protein PilP Machinery gene
  H044120014_RS04720 (BT148_RS04765) pilQ 1011460..1013556 (+) 2097 WP_010946666.1 type IV pilus secretin PilQ Machinery gene
  H044120014_RS04725 (BT148_RS04770) aroK 1014002..1014529 (+) 528 WP_010946667.1 shikimate kinase AroK -
  H044120014_RS04730 (BT148_RS04775) aroB 1014516..1015625 (+) 1110 WP_010946668.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22496.21 Da        Isoelectric Point: 5.3369

>NTDB_id=1145566 H044120014_RS04710 WP_014841277.1 1010273..1010872(+) (pilO) [Legionella pneumophila strain ST37 isolate ST37]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1145566 H044120014_RS04710 WP_014841277.1 1010273..1010872(+) (pilO) [Legionella pneumophila strain ST37 isolate ST37]
ATGGACACGGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGTATCAGTATACTGATAATTGGATTAGGGTATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCAGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATTGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACTTTGCATGAATTTAGTGTTGAGGGGGTGTCATCCGGAGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGCTATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

99.497

100

0.995
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

37.696

95.98

0.362

Multiple sequence alignment