Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BQ6063_RS07550 Genome accession   NZ_LT632615
Coordinates   1648537..1650261 (+) Length   574 a.a.
NCBI ID   WP_061690474.1    Uniprot ID   -
Organism   Legionella pneumophila strain ST23 isolate ST23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643537..1655261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BQ6063_RS07530 (EUL28_01489) - 1643890..1646412 (+) 2523 WP_027226542.1 putative bifunctional diguanylate cyclase/phosphodiesterase -
  BQ6063_RS07535 (EUL28_01490) - 1646414..1646983 (-) 570 WP_011213801.1 hypoxanthine-guanine phosphoribosyltransferase -
  BQ6063_RS07540 (EUL28_01491) - 1647026..1647352 (-) 327 WP_011213802.1 DUF1820 family protein -
  BQ6063_RS07545 (EUL28_01492) - 1647535..1648278 (-) 744 WP_011213803.1 methyltransferase domain-containing protein -
  BQ6063_RS07550 (EUL28_01493) pilB 1648537..1650261 (+) 1725 WP_061690474.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BQ6063_RS07555 (EUL28_01494) pilC 1650267..1651487 (+) 1221 WP_027226540.1 type II secretion system F family protein Machinery gene
  BQ6063_RS07560 (EUL28_01495) pilD 1651533..1652396 (+) 864 WP_011946525.1 prepilin peptidase Machinery gene
  BQ6063_RS07565 (EUL28_01496) - 1652410..1653615 (-) 1206 WP_013101470.1 cyclic nucleotide-binding domain-containing protein -
  BQ6063_RS07570 (EUL28_01497) - 1653723..1654316 (-) 594 WP_011213808.1 hypothetical protein -
  BQ6063_RS07575 (EUL28_01498) - 1654332..1654787 (-) 456 WP_027226538.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62712.27 Da        Isoelectric Point: 5.5861

>NTDB_id=1145531 BQ6063_RS07550 WP_061690474.1 1648537..1650261(+) (pilB) [Legionella pneumophila strain ST23 isolate ST23]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PVNLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVSGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1145531 BQ6063_RS07550 WP_061690474.1 1648537..1650261(+) (pilB) [Legionella pneumophila strain ST23 isolate ST23]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGACAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATATCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAATTGCATTGATGTGGGCACCATT
CCTGTCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGTCGCGGCACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAGGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCTCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTAGAAGGTTTGGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCACCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAAC
CTTATGAACGAGAATATCGAATCAGGTACAGGCAAGATGGTATTTTACATGAGGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAAGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGCACATGTCCAACCTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGTCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATCATG
GTTGGTGAGATACGTGATCTTGAAACAGCAGAGATTGCTATAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAATATGGGAATACCTACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGTGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAAGCTGATTTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGTGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTTGGGTTGTTTGAGGTTTTACCGATGACAAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGTTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATCTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

99.826

100

0.998
  pilB Acinetobacter baylyi ADP1

49.828

100

0.505
  pilB Acinetobacter baumannii D1279779

50.794

98.78

0.502
  pilB Vibrio cholerae strain A1552

50.926

94.077

0.479
  pilF Neisseria gonorrhoeae MS11

46.996

98.606

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

49.165

93.902

0.462
  pilB Vibrio campbellii strain DS40M4

47.576

97.038

0.462
  pilF Thermus thermophilus HB27

38.662

93.728

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

92.16

0.362

Multiple sequence alignment