Detailed information NTG1145523

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BQ6063_RS04780
• Genome accession: NZ_LT632615
• Coordinates: 1027543..1028607 (+)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila strain ST23 isolate ST23
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1022543..1033607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BQ6063_RS04770 (EUL28_00928)	-	1023583..1025967 (-)	2385	WP_061690536.1	penicillin-binding protein 1A	-
BQ6063_RS04775 (EUL28_00929)	ravI	1026119..1027126 (-)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
BQ6063_RS04780 (EUL28_00930)	pilM	1027543..1028607 (+)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
BQ6063_RS04785 (EUL28_00931)	pilN	1028611..1029159 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
BQ6063_RS04790 (EUL28_00932)	pilO	1029173..1029772 (+)	600	WP_011213355.1	type 4a pilus biogenesis protein PilO	Machinery gene
BQ6063_RS04795 (EUL28_00933)	pilP	1029769..1030353 (+)	585	WP_011213356.1	pilus assembly protein PilP	Machinery gene
BQ6063_RS04800 (EUL28_00934)	pilQ	1030357..1032453 (+)	2097	WP_027220941.1	type IV pilus secretin PilQ	Machinery gene
BQ6063_RS04805 (EUL28_00935)	aroK	1032899..1033426 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=1145523 BQ6063_RS04780 WP_011215094.1 1027543..1028607(+) (pilM) [Legionella pneumophila strain ST23 isolate ST23]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=1145523 BQ6063_RS04780 WP_011215094.1 1027543..1028607(+) (pilM) [Legionella pneumophila strain ST23 isolate ST23]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAGATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407