Detailed information NTG0114062

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: T303_RS00240
• Genome accession: NZ_CP006819
• Coordinates: 37320..37757 (-)
• Length: 145 a.a.
• NCBI ID: WP_011681686.1
• Uniprot ID: -
• Organism: Streptococcus thermophilus ASCC 1275
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 32320..42757

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
T303_RS00200 (T303_00200)	-	32383..33045 (-)	663	WP_011681679.1	type II CAAX endopeptidase family protein	-
T303_RS00205 (T303_00205)	-	33142..33585 (-)	444	WP_011681680.1	hypothetical protein	-
T303_RS11280 (T303_00210)	-	33659..33997 (-)	339	WP_224103197.1	CPBP family intramembrane glutamic endopeptidase	-
T303_RS11285	-	34041..34316 (-)	276	WP_011681681.1	hypothetical protein	-
T303_RS00220 (T303_00215)	-	34328..34526 (-)	199	Protein_41	helix-turn-helix transcriptional regulator	-
T303_RS00225 (T303_00220)	-	34775..35968 (-)	1194	WP_014608740.1	acetate kinase	-
T303_RS00230 (T303_00225)	comYH	36024..36980 (-)	957	WP_011681684.1	class I SAM-dependent methyltransferase	Machinery gene
T303_RS00235 (T303_00230)	comGG	37025..37342 (-)	318	WP_011681685.1	competence type IV pilus minor pilin ComGG	-
T303_RS00240 (T303_00235)	comYF	37320..37757 (-)	438	WP_011681686.1	competence type IV pilus minor pilin ComGF	Machinery gene
T303_RS00245 (T303_00240)	comGE	37741..38034 (-)	294	WP_024704238.1	competence type IV pilus minor pilin ComGE	-
T303_RS00250 (T303_00245)	comYD	38006..38434 (-)	429	WP_011226626.1	competence type IV pilus minor pilin ComGD	Machinery gene
T303_RS00255 (T303_00250)	comYC	38394..38720 (-)	327	WP_002946126.1	competence type IV pilus major pilin ComGC	Machinery gene
T303_RS00260 (T303_00255)	comYB	38717..39817 (-)	1101	WP_120764773.1	competence type IV pilus assembly protein ComGB	Machinery gene
T303_RS00265 (T303_00260)	comGA/cglA/cilD	39699..40640 (-)	942	WP_022096896.1	competence type IV pilus ATPase ComGA	Machinery gene
T303_RS00270 (T303_00265)	-	40721..41083 (-)	363	WP_014622006.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16375.85 Da
• Isoelectric Point: 8.4500

>NTDB_id=114062 T303_RS00240 WP_011681686.1 37320..37757(-) (comYF) [Streptococcus thermophilus ASCC 1275]
MYLSSKLRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVRQQENQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSASDFRKTNADGRGYQPMLFDVKSSKVSQKNQIVTIQVTLKNELQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=114062 T303_RS00240 WP_011681686.1 37320..37757(-) (comYF) [Streptococcus thermophilus ASCC 1275]
ATGTATCTAAGCAGTAAGCTGCGTGCCTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTCATTGCTGGCTCAGTTCA
AGTTTATCAAGGGTTAACCACTGTACTTGTGAGTAATGTTAAGCAGGTAAGACAACAAGAAAACCAAGATTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGATGTCAGTTGGTCAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTGGCTTTTGGTCTTTCAAGTGCCTCTGATTTTCGAAAAACAAATGCTGATGGTCGAGGTTATCAACCAAT
GCTTTTCGATGTCAAATCTAGTAAGGTAAGTCAAAAGAACCAGATAGTTACCATCCAAGTGACTCTTAAAAATGAACTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae D39	49.286	96.552	0.476
comGF/cglF	Streptococcus pneumoniae Rx1	49.286	96.552	0.476
comGF/cglF	Streptococcus pneumoniae R6	49.286	96.552	0.476
comGF/cglF	Streptococcus pneumoniae TIGR4	49.286	96.552	0.476
comGF/cglF	Streptococcus mitis NCTC 12261	47.518	97.241	0.462
comGF/cglF	Streptococcus mitis SK321	46.809	97.241	0.455
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434