Detailed information NTG1140432

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQM86_RS00685
• Genome accession: NZ_LS483407
• Coordinates: 104531..104965 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC13744
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99531..109965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM86_RS00655 (NCTC13744_00133)	-	101200..101565 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQM86_RS00660 (NCTC13744_00134)	comYA	101658..102596 (+)	939	WP_111674429.1	competence type IV pilus ATPase ComGA	Machinery gene
DQM86_RS00665 (NCTC13744_00135)	comYB	102532..103566 (+)	1035	WP_225793059.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQM86_RS00670 (NCTC13744_00136)	comYC	103568..103894 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQM86_RS00675 (NCTC13744_00137)	comGD	103869..104297 (+)	429	WP_023605232.1	competence type IV pilus minor pilin ComGD	-
DQM86_RS00680 (NCTC13744_00138)	comGE	104254..104538 (+)	285	WP_011284422.1	competence type IV pilus minor pilin ComGE	-
DQM86_RS00685 (NCTC13744_00139)	comYF	104531..104965 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQM86_RS00690 (NCTC13744_00140)	comGG	104949..105275 (+)	327	WP_002992739.1	competence type IV pilus minor pilin ComGG	-
DQM86_RS00695 (NCTC13744_00141)	comYH	105373..106326 (+)	954	WP_002987790.1	class I SAM-dependent methyltransferase	Machinery gene
DQM86_RS00700 (NCTC13744_00142)	-	106385..107581 (+)	1197	WP_002986533.1	acetate kinase	-
DQM86_RS00705	-	107768..108076 (+)	309	WP_011017251.1	hypothetical protein	-
DQM86_RS00710 (NCTC13744_00143)	proC	108159..108929 (-)	771	WP_002986527.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=1140432 DQM86_RS00685 WP_002992738.1 104531..104965(+) (comYF) [Streptococcus pyogenes strain NCTC13744]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1140432 DQM86_RS00685 WP_002992738.1 104531..104965(+) (comYF) [Streptococcus pyogenes strain NCTC13744]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396