Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   DQM35_RS00665 Genome accession   NZ_LS483338
Coordinates   102784..103818 (+) Length   344 a.a.
NCBI ID   WP_225793059.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC12064     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97784..108818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM35_RS00655 (NCTC12064_00133) - 101466..101831 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQM35_RS00660 (NCTC12064_00134) comGA 101924..102848 (+) 925 Protein_81 competence type IV pilus ATPase ComGA -
  DQM35_RS00665 (NCTC12064_00135) comYB 102784..103818 (+) 1035 WP_225793059.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQM35_RS00670 (NCTC12064_00136) comYC 103820..104146 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DQM35_RS00675 (NCTC12064_00137) comGD 104121..104549 (+) 429 WP_023605232.1 competence type IV pilus minor pilin ComGD -
  DQM35_RS00680 (NCTC12064_00138) comGE 104506..104790 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  DQM35_RS00685 (NCTC12064_00139) comYF 104783..105217 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQM35_RS00690 (NCTC12064_00140) comGG 105201..105527 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  DQM35_RS00695 (NCTC12064_00141) comYH 105625..106578 (+) 954 WP_023610092.1 class I SAM-dependent methyltransferase Machinery gene
  DQM35_RS00700 (NCTC12064_00142) - 106637..107833 (+) 1197 WP_038431193.1 acetate kinase -
  DQM35_RS00705 - 108020..108328 (+) 309 WP_032467031.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39646.12 Da        Isoelectric Point: 9.7085

>NTDB_id=1137282 DQM35_RS00665 WP_225793059.1 102784..103818(+) (comYB) [Streptococcus pyogenes strain NCTC12064]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMAILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1137282 DQM35_RS00665 WP_225793059.1 102784..103818(+) (comYB) [Streptococcus pyogenes strain NCTC12064]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGGCTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment