Detailed information NTG1137284

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQM35_RS00685
• Genome accession: NZ_LS483338
• Coordinates: 104783..105217 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC12064
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99783..110217

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM35_RS00655 (NCTC12064_00133)	-	101466..101831 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQM35_RS00660 (NCTC12064_00134)	comGA	101924..102848 (+)	925	Protein_81	competence type IV pilus ATPase ComGA	-
DQM35_RS00665 (NCTC12064_00135)	comYB	102784..103818 (+)	1035	WP_225793059.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQM35_RS00670 (NCTC12064_00136)	comYC	103820..104146 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQM35_RS00675 (NCTC12064_00137)	comGD	104121..104549 (+)	429	WP_023605232.1	competence type IV pilus minor pilin ComGD	-
DQM35_RS00680 (NCTC12064_00138)	comGE	104506..104790 (+)	285	WP_011284422.1	competence type IV pilus minor pilin ComGE	-
DQM35_RS00685 (NCTC12064_00139)	comYF	104783..105217 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQM35_RS00690 (NCTC12064_00140)	comGG	105201..105527 (+)	327	WP_002992739.1	competence type IV pilus minor pilin ComGG	-
DQM35_RS00695 (NCTC12064_00141)	comYH	105625..106578 (+)	954	WP_023610092.1	class I SAM-dependent methyltransferase	Machinery gene
DQM35_RS00700 (NCTC12064_00142)	-	106637..107833 (+)	1197	WP_038431193.1	acetate kinase	-
DQM35_RS00705	-	108020..108328 (+)	309	WP_032467031.1	hypothetical protein	-
DQM35_RS00710 (NCTC12064_00143)	proC	108411..109181 (-)	771	WP_002992745.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=1137284 DQM35_RS00685 WP_002992738.1 104783..105217(+) (comYF) [Streptococcus pyogenes strain NCTC12064]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1137284 DQM35_RS00685 WP_002992738.1 104783..105217(+) (comYF) [Streptococcus pyogenes strain NCTC12064]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396