Detailed information NTG1137005

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQL30_RS00790
• Genome accession: NZ_LS483333
• Coordinates: 117998..118432 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC12048
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 112998..123432

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL30_RS00760 (NCTC12048_00154)	-	114679..115044 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQL30_RS00765 (NCTC12048_00155)	comYA	115137..116075 (+)	939	WP_111713563.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL30_RS00770 (NCTC12048_00156)	comYB	116011..117045 (+)	1035	WP_227868709.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQL30_RS00775 (NCTC12048_00157)	comGC	117047..117361 (+)	315	WP_111713564.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL30_RS00780 (NCTC12048_00158)	comGD	117336..117764 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
DQL30_RS00785 (NCTC12048_00159)	comGE	117721..118005 (+)	285	WP_011284422.1	competence type IV pilus minor pilin ComGE	-
DQL30_RS00790 (NCTC12048_00160)	comYF	117998..118432 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL30_RS00795 (NCTC12048_00161)	comGG	118416..118742 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
DQL30_RS00800 (NCTC12048_00162)	comYH	118840..119793 (+)	954	WP_002987790.1	class I SAM-dependent methyltransferase	Machinery gene
DQL30_RS00805 (NCTC12048_00163)	-	119852..121048 (+)	1197	WP_010921803.1	acetate kinase	-
DQL30_RS00810	-	121235..121543 (+)	309	Protein_112	hypothetical protein	-
DQL30_RS00815 (NCTC12048_00164)	proC	121626..122396 (-)	771	WP_038433852.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=1137005 DQL30_RS00790 WP_002992738.1 117998..118432(+) (comYF) [Streptococcus pyogenes strain NCTC12048]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1137005 DQL30_RS00790 WP_002992738.1 117998..118432(+) (comYF) [Streptococcus pyogenes strain NCTC12048]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396