Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   DQL15_RS00675 Genome accession   NZ_LS483323
Coordinates   103457..104491 (+) Length   344 a.a.
NCBI ID   WP_223845212.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8300     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 98457..109491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL15_RS00665 (NCTC8300_00134) - 102125..102490 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQL15_RS00670 (NCTC8300_00135) comYA 102583..103521 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL15_RS00675 (NCTC8300_00136) comYB 103457..104491 (+) 1035 WP_223845212.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL15_RS00680 (NCTC8300_00137) comYC 104493..104819 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL15_RS00685 (NCTC8300_00138) comGD 104794..105222 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL15_RS00690 (NCTC8300_00139) comGE 105179..105463 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL15_RS00695 (NCTC8300_00140) comYF 105456..105890 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL15_RS00700 (NCTC8300_00141) comGG 105874..106200 (+) 327 WP_002987787.1 competence type IV pilus minor pilin ComGG -
  DQL15_RS00705 (NCTC8300_00142) comYH 106298..107251 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DQL15_RS00710 (NCTC8300_00143) - 107310..108506 (+) 1197 WP_002987791.1 acetate kinase -
  DQL15_RS00715 - 108693..109001 (+) 309 WP_011284425.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39734.23 Da        Isoelectric Point: 9.7085

>NTDB_id=1136454 DQL15_RS00675 WP_223845212.1 103457..104491(+) (comYB) [Streptococcus pyogenes strain NCTC8300]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWSTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGVELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1136454 DQL15_RS00675 WP_223845212.1 103457..104491(+) (comYB) [Streptococcus pyogenes strain NCTC8300]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGAGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGTTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.462

94.477

0.401

Multiple sequence alignment