Detailed information NTG1135597

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: DQL80_RS07750
• Genome accession: NZ_LS483302
• Coordinates: 1570466..1570963 (-)
• Length: 165 a.a.
• NCBI ID: WP_001796472.1
• Uniprot ID: A0A5F0HJ55
• Organism: Staphylococcus aureus strain NCTC8726
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1565466..1575963

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL80_RS07725 (NCTC8726_01467)	gcvPB	1565628..1567100 (-)	1473	WP_000202176.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
DQL80_RS07730 (NCTC8726_01468)	gcvPA	1567093..1568439 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
DQL80_RS07735 (NCTC8726_01469)	gcvT	1568459..1569550 (-)	1092	WP_000093344.1	glycine cleavage system aminomethyltransferase GcvT	-
DQL80_RS07740	-	1569709..1570233 (-)	525	WP_111680347.1	shikimate kinase	-
DQL80_RS07745	-	1570223..1570369 (-)	147	WP_001789879.1	hypothetical protein	-
DQL80_RS07750 (NCTC8726_01471)	comGF	1570466..1570963 (-)	498	WP_001796472.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL80_RS07755 (NCTC8726_01472)	comGE	1570881..1571180 (-)	300	WP_172452442.1	competence protein ComGE	Machinery gene
DQL80_RS07760 (NCTC8726_01473)	comGD	1571167..1571613 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
DQL80_RS07765 (NCTC8726_01474)	comGC	1571591..1571902 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL80_RS07770 (NCTC8726_01475)	comGB	1571916..1572986 (-)	1071	WP_000776414.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQL80_RS07775 (NCTC8726_01476)	comGA	1572958..1573932 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL80_RS07780 (NCTC8726_01477)	-	1573984..1574607 (-)	624	WP_001223009.1	MBL fold metallo-hydrolase	-
DQL80_RS07785 (NCTC8726_01478)	-	1574604..1574933 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
DQL80_RS07790 (NCTC8726_01479)	-	1574933..1575919 (-)	987	WP_000161307.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19169.89 Da
• Isoelectric Point: 10.1358

>NTDB_id=1135597 DQL80_RS07750 WP_001796472.1 1570466..1570963(-) (comGF) [Staphylococcus aureus strain NCTC8726]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1135597 DQL80_RS07750 WP_001796472.1 1570466..1570963(-) (comGF) [Staphylococcus aureus strain NCTC8726]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A5F0HJ55

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	95.758	100	0.958