Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DQL80_RS07775 Genome accession   NZ_LS483302
Coordinates   1572958..1573932 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain NCTC8726     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1567958..1578932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL80_RS07735 (NCTC8726_01469) gcvT 1568459..1569550 (-) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  DQL80_RS07740 - 1569709..1570233 (-) 525 WP_111680347.1 shikimate kinase -
  DQL80_RS07745 - 1570223..1570369 (-) 147 WP_001789879.1 hypothetical protein -
  DQL80_RS07750 (NCTC8726_01471) comGF 1570466..1570963 (-) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL80_RS07755 (NCTC8726_01472) comGE 1570881..1571180 (-) 300 WP_172452442.1 competence protein ComGE Machinery gene
  DQL80_RS07760 (NCTC8726_01473) comGD 1571167..1571613 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  DQL80_RS07765 (NCTC8726_01474) comGC 1571591..1571902 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL80_RS07770 (NCTC8726_01475) comGB 1571916..1572986 (-) 1071 WP_000776414.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL80_RS07775 (NCTC8726_01476) comGA 1572958..1573932 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL80_RS07780 (NCTC8726_01477) - 1573984..1574607 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  DQL80_RS07785 (NCTC8726_01478) - 1574604..1574933 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DQL80_RS07790 (NCTC8726_01479) - 1574933..1575919 (-) 987 WP_000161307.1 ROK family glucokinase -
  DQL80_RS07795 - 1575916..1576119 (-) 204 WP_000087561.1 YqgQ family protein -
  DQL80_RS07800 (NCTC8726_01480) - 1576100..1577563 (-) 1464 WP_111680349.1 rhomboid family protein -
  DQL80_RS07805 (NCTC8726_01481) - 1577575..1578114 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  DQL80_RS07810 (NCTC8726_01482) rpmG 1578323..1578472 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=1135602 DQL80_RS07775 WP_000697220.1 1572958..1573932(-) (comGA) [Staphylococcus aureus strain NCTC8726]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1135602 DQL80_RS07775 WP_000697220.1 1572958..1573932(-) (comGA) [Staphylococcus aureus strain NCTC8726]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment