Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JMX72_RS26680 Genome accession   NZ_LR898867
Coordinates   5690497..5692200 (+) Length   567 a.a.
NCBI ID   WP_003116249.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain MINF_3A isolate MINF_3A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5670087..5689665 5690497..5692200 flank 832


Gene organization within MGE regions


Location: 5670087..5692200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMX72_RS26560 - 5670087..5671301 (+) 1215 WP_031653965.1 site-specific integrase -
  JMX72_RS26565 - 5671293..5672039 (-) 747 WP_044261824.1 hypothetical protein -
  JMX72_RS26570 - 5672252..5672395 (+) 144 WP_315986167.1 helix-turn-helix domain-containing protein -
  JMX72_RS26575 - 5672392..5672808 (+) 417 WP_031653968.1 hypothetical protein -
  JMX72_RS26580 - 5672801..5673034 (+) 234 WP_201720838.1 hypothetical protein -
  JMX72_RS26585 - 5673031..5673279 (+) 249 WP_031653970.1 hypothetical protein -
  JMX72_RS26590 - 5673276..5673581 (+) 306 WP_034010508.1 hypothetical protein -
  JMX72_RS32610 - 5673584..5674021 (+) 438 WP_309039959.1 hypothetical protein -
  JMX72_RS26600 - 5674060..5674527 (+) 468 WP_031629313.1 hypothetical protein -
  JMX72_RS26605 - 5674534..5675484 (+) 951 WP_223716474.1 DUF3631 domain-containing protein -
  JMX72_RS26610 - 5675484..5675780 (+) 297 WP_034010502.1 hypothetical protein -
  JMX72_RS26615 - 5675836..5676732 (+) 897 WP_023082017.1 hypothetical protein -
  JMX72_RS26620 - 5677654..5677818 (+) 165 WP_153519866.1 hypothetical protein -
  JMX72_RS26625 - 5677835..5679427 (+) 1593 WP_023082018.1 anti-phage dCTP deaminase -
  JMX72_RS26630 - 5680230..5680736 (-) 507 WP_034010500.1 GNAT family N-acetyltransferase -
  JMX72_RS26635 - 5681500..5681769 (+) 270 WP_225007575.1 hypothetical protein -
  JMX72_RS26640 - 5681769..5682005 (+) 237 WP_003096116.1 hypothetical protein -
  JMX72_RS26645 - 5682139..5682471 (-) 333 WP_079384197.1 hypothetical protein -
  JMX72_RS26650 - 5682464..5685133 (-) 2670 WP_034049795.1 RHS repeat domain-containing protein -
  JMX72_RS26655 - 5685148..5686296 (-) 1149 WP_079388041.1 DUF6531 domain-containing protein -
  JMX72_RS26660 - 5688482..5688724 (+) 243 WP_023109353.1 hypothetical protein -
  JMX72_RS26670 - 5688967..5689665 (-) 699 WP_223677410.1 hypothetical protein -
  JMX72_RS26675 - 5689729..5690268 (-) 540 WP_033938465.1 pilin -
  JMX72_RS26680 pilB 5690497..5692200 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=1134801 JMX72_RS26680 WP_003116249.1 5690497..5692200(+) (pilB) [Pseudomonas aeruginosa strain MINF_3A isolate MINF_3A]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1134801 JMX72_RS26680 WP_003116249.1 5690497..5692200(+) (pilB) [Pseudomonas aeruginosa strain MINF_3A isolate MINF_3A]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAAAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.582
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376

Multiple sequence alignment