Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H5027_RS04845 Genome accession   NZ_LR861807
Coordinates   1164805..1166541 (+) Length   578 a.a.
NCBI ID   WP_053051303.1    Uniprot ID   A0A8E4ENF3
Organism   Xanthomonas arboricola pv. juglandis isolate 3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1146863..1164770 1164805..1166541 flank 35


Gene organization within MGE regions


Location: 1146863..1166541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5027_RS04755 (XSP_000951) - 1146863..1147423 (-) 561 WP_102596783.1 hypothetical protein -
  H5027_RS22610 - 1147940..1148089 (-) 150 WP_256468814.1 hypothetical protein -
  H5027_RS04765 (XSP_000953) - 1148158..1148670 (+) 513 WP_049805061.1 ImmA/IrrE family metallo-endopeptidase -
  H5027_RS04770 (XSP_000954) - 1149245..1149631 (+) 387 WP_026064907.1 hypothetical protein -
  H5027_RS04775 (XSP_000955) - 1149720..1150112 (+) 393 WP_023904915.1 H-NS family nucleoid-associated regulatory protein -
  H5027_RS04780 (XSP_000956) glgX 1150784..1152916 (-) 2133 WP_053051310.1 glycogen debranching protein GlgX -
  H5027_RS04785 (XSP_000957) rimK 1153407..1154312 (+) 906 WP_166767257.1 30S ribosomal protein S6--L-glutamate ligase -
  H5027_RS22645 (XSP_000958) - 1154503..1154980 (+) 478 Protein_937 hypothetical protein -
  H5027_RS04795 (XSP_000959) - 1155008..1155685 (+) 678 WP_006448355.1 response regulator transcription factor -
  H5027_RS04800 (XSP_000960) - 1155678..1157012 (+) 1335 WP_016904089.1 HAMP domain-containing sensor histidine kinase -
  H5027_RS04805 (XSP_000961) - 1157116..1157264 (-) 149 Protein_940 SymE family type I addiction module toxin -
  H5027_RS04810 (XSP_000962) coaE 1157364..1157987 (-) 624 WP_053051308.1 dephospho-CoA kinase -
  H5027_RS04815 (XSP_000963) - 1158001..1158864 (-) 864 WP_016849610.1 A24 family peptidase -
  H5027_RS04820 (XSP_000964) pilC 1158871..1160130 (-) 1260 WP_053051318.1 type II secretion system F family protein Machinery gene
  H5027_RS04825 (XSP_000965) pilA 1160484..1160909 (+) 426 WP_053051307.1 pilin Machinery gene
  H5027_RS04830 (XSP_000966) - 1161045..1162634 (+) 1590 WP_053051306.1 phosphoethanolamine transferase -
  H5027_RS04835 (XSP_000967) - 1162705..1163274 (+) 570 WP_231103946.1 hypothetical protein -
  H5027_RS04840 (XSP_000968) - 1163244..1164770 (+) 1527 WP_053051304.1 hypothetical protein -
  H5027_RS04845 (XSP_000969) pilB 1164805..1166541 (+) 1737 WP_053051303.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62620.72 Da        Isoelectric Point: 5.6044

>NTDB_id=1132472 H5027_RS04845 WP_053051303.1 1164805..1166541(+) (pilB) [Xanthomonas arboricola pv. juglandis isolate 3]
MNSVATANLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAERKLVSAAQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSSLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1132472 H5027_RS04845 WP_053051303.1 1164805..1166541(+) (pilB) [Xanthomonas arboricola pv. juglandis isolate 3]
ATGAATTCAGTAGCTACCGCCAACCTCGTTGGTATTACCGGCATCGCTCGTCGCCTTGTGCAGGATGGTGCCCTGGAGGA
AGCCGCTGCGCGGACGGCAATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTTCCTCAGTGGTTTGCCGAAAGAAAACTGG
TGTCGGCGGCACAACTGGCGGCAGCCAATGCCGTGGAATTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCCAAT
CAGAACGCGGTCAAGCTGGTCAGTGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTTCATACCAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAACGCGTCGTTGGGCTCGTCGCTAGGG
GACGATGACGAGGGGATGGGCGACCTGGACGTGTCGGCCGGTGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGGATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCG
GTAAAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGACATTGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACGTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCATAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTACACGGCACTCGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGTTTGCCCG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCTTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGAGCCTGCCC
GAGCATGCGTTGCTGGCCGAAGGCTTCACGCCTGCGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGTACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCACAGAGTATCGGCATCCGCGACCTACGCCAGTCGGCGCTGGTC
AAGGCCGCGCACGGGGTAACCAGCCTGGCCGAGATCAACCGGGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.27

0.547
  pilB Acinetobacter baylyi ADP1

55.634

98.27

0.547
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilB Vibrio cholerae strain A1552

49.132

99.654

0.49
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilF Thermus thermophilus HB27

40.192

89.965

0.362

Multiple sequence alignment