Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FGL10_RS07160 Genome accession   NZ_LR590477
Coordinates   1362658..1364334 (-) Length   558 a.a.
NCBI ID   WP_003710978.1    Uniprot ID   D0WCG9
Organism   Neisseria lactamica strain NCTC10617     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1357658..1369334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL10_RS07140 (NCTC10617_01240) pilG 1359571..1360803 (+) 1233 WP_003710974.1 type 4 pilus assembly protein PilG Machinery gene
  FGL10_RS07145 (NCTC10617_01241) pilD 1360877..1361737 (+) 861 WP_003710975.1 prepilin peptidase Machinery gene
  FGL10_RS07150 (NCTC10617_01242) coaE 1361739..1362371 (+) 633 WP_003710976.1 dephospho-CoA kinase -
  FGL10_RS07155 (NCTC10617_01243) - 1362364..1362573 (+) 210 WP_003710977.1 DNA gyrase inhibitor YacG -
  FGL10_RS07160 (NCTC10617_01244) pilF 1362658..1364334 (-) 1677 WP_003710978.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FGL10_RS07175 (NCTC10617_01246) - 1364966..1365412 (-) 447 WP_003710982.1 DUF441 domain-containing protein -
  FGL10_RS07180 (NCTC10617_01247) - 1365417..1366391 (-) 975 WP_003710984.1 polyprenyl synthetase family protein -
  FGL10_RS07190 (NCTC10617_01248) rplU 1366615..1366923 (+) 309 WP_002216394.1 50S ribosomal protein L21 -
  FGL10_RS07195 (NCTC10617_01249) rpmA 1366949..1367221 (+) 273 WP_002212328.1 50S ribosomal protein L27 -
  FGL10_RS07200 (NCTC10617_01250) ubiM 1367455..1368639 (+) 1185 WP_003714013.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  FGL10_RS07205 (NCTC10617_01251) rpmG 1368828..1368983 (-) 156 WP_003689824.1 50S ribosomal protein L33 -
  FGL10_RS07210 (NCTC10617_01252) rpmB 1369015..1369248 (-) 234 WP_002216391.1 50S ribosomal protein L28 -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62047.15 Da        Isoelectric Point: 5.4107

>NTDB_id=1126939 FGL10_RS07160 WP_003710978.1 1362658..1364334(-) (pilF) [Neisseria lactamica strain NCTC10617]
MSVGLLRTLVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSVLDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRDNKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEEQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPIDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNEKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEIGFTDEDLAKDWKLY
HAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1126939 FGL10_RS07160 WP_003710978.1 1362658..1364334(-) (pilF) [Neisseria lactamica strain NCTC10617]
ATGAGCGTAGGTTTGCTGAGAACTTTGGTTCAAAACCAAGTGGTTACTGTCGAACAGGCCGAACACTATTATAAGGAGTC
GCAGGCGGGTAAAGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCTAAATCCCTTGCGGCACTGATTGCCA
GGGTGTTCAGTTATTCGGTTCTTGATTTGCGCCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAAGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGACAATAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTGTCTGCCGCAGGGATTGCGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGGTCGAGATCGACATCGCTGCTTCAGGAGCTTGGGGAGGAGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAATGAGGAGGCGGAGGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATTCATTTTGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGCGTGGACGGGCAGCTTCGCGAGGTGG
TTCAGCCGCCTATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTGATGTCGCGTTTGGATATTTCCGAAAAACGC
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTATCGATTTCCGCGTCAGCACTTTGCCGAC
GCTGTTTGGCGAAAAGGTTGTGATGCGGATTTTGAACTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGCTGTTGGAAGCGATTCATCGTCCTTACGGTATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTCAACGTCAATGAGAAGCAGGGTCTGACTTTTGCCGCTGCTTTAAAGTCTTTCC
TGCGTCAGGATCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGTTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCTTCTGCTCTGAAGGAAATCGGCTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CATGCCGTCGGTTGCGACCGTTGCCGGGGTCAGGGTTACAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0WCG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

97.849

100

0.978
  pilB Acinetobacter baumannii D1279779

50.355

100

0.509
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.703

100

0.484
  pilB Vibrio cholerae strain A1552

45.552

100

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio campbellii strain DS40M4

44.149

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.179

96.057

0.376

Multiple sequence alignment