Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   E0F40_RS10005 Genome accession   NZ_LR216065
Coordinates   1861785..1862246 (-) Length   153 a.a.
NCBI ID   WP_000250529.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1856785..1867246
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F40_RS09975 (SAMEA3714487_01891) - 1857097..1857921 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  E0F40_RS09980 (SAMEA3714487_01892) rnpA 1857896..1858267 (-) 372 WP_000739246.1 ribonuclease P protein component -
  E0F40_RS09985 (SAMEA3714487_01893) - 1858415..1859605 (-) 1191 WP_000167755.1 acetate kinase -
  E0F40_RS09990 (SAMEA3714487_01894) comYH 1859656..1860609 (-) 954 WP_000345084.1 class I SAM-dependent methyltransferase Machinery gene
  E0F40_RS09995 - 1860670..1861257 (-) 588 Protein_1865 class I SAM-dependent methyltransferase -
  E0F40_RS10000 (SAMEA3714487_01897) comGG/cglG 1861394..1861807 (-) 414 WP_000265617.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F40_RS10005 (SAMEA3714487_01898) comGF/cglF 1861785..1862246 (-) 462 WP_000250529.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F40_RS10010 (SAMEA3714487_01899) comGE/cglE 1862209..1862511 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F40_RS10015 (SAMEA3714487_01900) comGD/cglD 1862474..1862878 (-) 405 WP_000588002.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F40_RS10020 (SAMEA3714487_01901) comGC/cglC 1862871..1863137 (-) 267 WP_000962026.1 competence type IV pilus major pilin ComGC Machinery gene
  E0F40_RS10025 (SAMEA3714487_01902) - 1863139..1863396 (-) 258 WP_000698513.1 hypothetical protein -
  E0F40_RS10030 (SAMEA3714487_01903) - 1863667..1864623 (-) 957 WP_054385177.1 N-acetylmuramoyl-L-alanine amidase -
  E0F40_RS10035 (SAMEA3714487_01904) - 1864627..1864959 (-) 333 WP_001186216.1 phage holin -
  E0F40_RS10040 (SAMEA3714487_01905) - 1864963..1865262 (-) 300 WP_050203193.1 hypothetical protein -
  E0F40_RS10045 (SAMEA3714487_01906) - 1865271..1865621 (-) 351 WP_000852245.1 hypothetical protein -
  E0F40_RS10050 (SAMEA3714487_01907) - 1865624..1865827 (-) 204 WP_001091112.1 hypothetical protein -
  E0F40_RS12520 (SAMEA3714487_01908) - 1865808..1865924 (-) 117 WP_001063633.1 hypothetical protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17851.34 Da        Isoelectric Point: 6.4832

>NTDB_id=1126370 E0F40_RS10005 WP_000250529.1 1861785..1862246(-) (comGF/cglF) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1126370 E0F40_RS10005 WP_000250529.1 1861785..1862246(-) (comGF/cglF) [Streptococcus pneumoniae strain GPSC18 substr. ST13 isolate 55896440-41bd-11e5-998e-3c4a9275d6c6]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAAGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae D39

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae R6

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae TIGR4

94.118

100

0.941
  comGF/cglF Streptococcus mitis SK321

92.81

100

0.928
  comGF/cglF Streptococcus mitis NCTC 12261

91.503

100

0.915

Multiple sequence alignment