Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFC/cflB   Type   Machinery gene
Locus tag   E0F32_RS10990 Genome accession   NZ_LR216050
Coordinates   2024336..2024998 (-) Length   220 a.a.
NCBI ID   WP_000649971.1    Uniprot ID   A0A0B7MFB3
Organism   Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2019336..2029998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F32_RS10965 (SAMEA3431333_02058) - 2019486..2019752 (-) 267 WP_001278167.1 Veg family protein -
  E0F32_RS10970 (SAMEA3431333_02059) dnaB 2019754..2021106 (-) 1353 WP_000852486.1 replicative DNA helicase -
  E0F32_RS10975 (SAMEA3431333_02060) rplI 2021150..2021602 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  E0F32_RS10980 (SAMEA3431333_02061) - 2021599..2023572 (-) 1974 WP_000715124.1 DHH family phosphoesterase -
  E0F32_RS10985 (SAMEA3431333_02062) hpf 2023708..2024256 (-) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  E0F32_RS10990 (SAMEA3431333_02063) comFC/cflB 2024336..2024998 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  E0F32_RS10995 (SAMEA3431333_02064) comFA/cflA 2024995..2026293 (-) 1299 WP_000867601.1 DEAD/DEAH box helicase Machinery gene
  E0F32_RS11000 (SAMEA3431333_02065) - 2026349..2026984 (+) 636 WP_050072350.1 YigZ family protein -
  E0F32_RS11010 (SAMEA3431333_02066) cysK 2027269..2028189 (+) 921 WP_000029866.1 cysteine synthase A -
  E0F32_RS11020 (SAMEA3431333_02067) - 2028264..2028802 (-) 539 Protein_2052 transposase -

Sequence

Protein


Download         Length: 220 a.a.        Molecular weight: 25241.10 Da        Isoelectric Point: 7.3688

>NTDB_id=1126156 E0F32_RS10990 WP_000649971.1 2024336..2024998(-) (comFC/cflB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEENCPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQ
AMKDFFSRYKFDGDFLLRKVFASFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSLVR

Nucleotide


Download         Length: 663 bp        

>NTDB_id=1126156 E0F32_RS10990 WP_000649971.1 2024336..2024998(-) (comFC/cflB) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGAAGTGCTTGTTATGTGGGCAGACTATGAAGACTGTTTTAACTTTTAGTAGTCTCTTACTTCTGAGGAATGATGACTC
TTGTCTTTGTTCAGACTGTGATTCTACTTTTGAAAGAATTGGGGAAGAGAACTGTCCAAATTGTATGAAAACAGAGTTGT
CAACAAAGTGTCAAGATTGTCAACTTTGGTGTAAAGAGGGAGTTGAAGTCAGTCATAGAGCGATTTTTACTTACAATCAA
GCTATGAAGGATTTTTTCAGTCGGTATAAGTTTGATGGAGACTTCCTGTTAAGAAAAGTTTTCGCTTCATTTTTAAGTGA
GGAGTTGAAAAAGTACAAAGAGTATCAATTTGTTGTAATTCCCCTAAGTCCTGATAGATATGCTAATAGAGGATTTAATC
AGGTTGAGGGCTTGGTAGAGGCAGCAGGCTTTGAGTATCTGGATTTATTAGAGAAAAGAGAAGAGAGAGCCAGTTCTTCT
AAAAATCGTTCAGAGCGCTTGGGGACAGAACTTCCTTTCTTTATTAAAAGTGGAGTCACTATTCCTAAAAAAATCCTACT
TATAGATGATATCTATACTACAGGAGCAACTATAAATCGTGTTAAGAAACTGTTGGAAGAAGCTGGTGCTAAGGATGTAA
AAACATTTTCCCTTGTAAGATGA

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(153-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7MFB3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFC/cflB Streptococcus pneumoniae TIGR4

100

100

1
  comFC/cflB Streptococcus pneumoniae Rx1

99.545

100

0.995
  comFC/cflB Streptococcus pneumoniae D39

99.545

100

0.995
  comFC/cflB Streptococcus pneumoniae R6

99.545

100

0.995
  comFC/cflB Streptococcus mitis NCTC 12261

91.818

100

0.918
  comFC/cflB Streptococcus mitis SK321

91.364

100

0.914

Multiple sequence alignment