Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   E0F32_RS10995 Genome accession   NZ_LR216050
Coordinates   2024995..2026293 (-) Length   432 a.a.
NCBI ID   WP_000867601.1    Uniprot ID   A0A0B7LBE5
Organism   Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2019995..2031293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F32_RS10975 (SAMEA3431333_02060) rplI 2021150..2021602 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  E0F32_RS10980 (SAMEA3431333_02061) - 2021599..2023572 (-) 1974 WP_000715124.1 DHH family phosphoesterase -
  E0F32_RS10985 (SAMEA3431333_02062) hpf 2023708..2024256 (-) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  E0F32_RS10990 (SAMEA3431333_02063) comFC/cflB 2024336..2024998 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  E0F32_RS10995 (SAMEA3431333_02064) comFA/cflA 2024995..2026293 (-) 1299 WP_000867601.1 DEAD/DEAH box helicase Machinery gene
  E0F32_RS11000 (SAMEA3431333_02065) - 2026349..2026984 (+) 636 WP_050072350.1 YigZ family protein -
  E0F32_RS11010 (SAMEA3431333_02066) cysK 2027269..2028189 (+) 921 WP_000029866.1 cysteine synthase A -
  E0F32_RS11020 (SAMEA3431333_02067) - 2028264..2028802 (-) 539 Protein_2052 transposase -
  E0F32_RS11025 (SAMEA3431333_02068) - 2029046..2030392 (-) 1347 WP_001809444.1 IS1380-like element ISSpn5 family transposase -
  E0F32_RS11030 - 2030558..2030790 (+) 233 Protein_2054 IS630 family transposase -
  E0F32_RS11035 (SAMEA3431333_02069) tnpB 2030931..2031254 (-) 324 WP_000973414.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49562.56 Da        Isoelectric Point: 8.5992

>NTDB_id=1126157 E0F32_RS10995 WP_000867601.1 2024995..2026293(-) (comFA/cflA) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1126157 E0F32_RS10995 WP_000867601.1 2024995..2026293(-) (comFA/cflA) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGTATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7LBE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

100

100

1
  comFA/cflA Streptococcus pneumoniae D39

100

100

1
  comFA/cflA Streptococcus pneumoniae R6

100

100

1
  comFA/cflA Streptococcus pneumoniae TIGR4

99.769

100

0.998
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.451

94.444

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389

Multiple sequence alignment