Detailed information NTG0112314

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: S7S_RS01685
• Genome accession: NZ_CP004387
• Coordinates: 348781..349848 (+)
• Length: 355 a.a.
• NCBI ID: WP_041025862.1
• Uniprot ID: A0A0B4XI61
• Organism: Isoalcanivorax pacificus W11-5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 343781..354848

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
S7S_RS01670 (S7S_01670)	rpmE	344041..344262 (+)	222	WP_008736397.1	50S ribosomal protein L31	-
S7S_RS01675 (S7S_01675)	-	344402..345679 (+)	1278	WP_041025861.1	malic enzyme-like NAD(P)-binding protein	-
S7S_RS01680 (S7S_01680)	-	345957..348575 (-)	2619	WP_052269180.1	penicillin-binding protein 1A	-
S7S_RS01685 (S7S_01685)	pilM	348781..349848 (+)	1068	WP_041025862.1	pilus assembly protein PilM	Machinery gene
S7S_RS01690 (S7S_01690)	-	349845..350414 (+)	570	WP_041025863.1	PilN domain-containing protein	-
S7S_RS01695 (S7S_01695)	-	350411..351094 (+)	684	WP_041025864.1	type 4a pilus biogenesis protein PilO	-
S7S_RS01700 (S7S_01700)	-	351120..351644 (+)	525	WP_238582922.1	pilus assembly protein PilP	-
S7S_RS01705 (S7S_01705)	pilQ	351691..353769 (+)	2079	WP_052269181.1	type IV pilus secretin PilQ	Machinery gene
S7S_RS01710 (S7S_01710)	aroK	353779..354306 (+)	528	WP_041025867.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 38538.88 Da
• Isoelectric Point: 4.2861

>NTDB_id=112314 S7S_RS01685 WP_041025862.1 348781..349848(+) (pilM) [Isoalcanivorax pacificus W11-5]
MLPFLSQKAQRVLGIDISTTAVKLLELSKSGDRYRVDSYGVEPLPPNAVVEKTVNDEPSVGETIARLISRARPGSKLAAV
AVPGSAVITKTIEMDASLDEDEMENQLKVEADQYIPFPLDEVRMDFCVMGQVEGNADKVEVLLVASRTENVQRRVDCIEI
GGLKAGGVDVEAYAMERAFQLVLPQLPHGDELETVAVFDIGATMTTLNVLHQGRTVYTREQLFGGKQLTEEIQRRYGISM
EEAGLAKKTGELPDDYESEVLQPFKEAIVQQINRSLQFFYGSTQFNEVDYILLAGGSASIAGLPELVQEKIGASCSVANP
FTDMALASKVSAASIANDAPALMIACGLALRSFEE

Nucleotide

• Length: 1068 bp

>NTDB_id=112314 S7S_RS01685 WP_041025862.1 348781..349848(+) (pilM) [Isoalcanivorax pacificus W11-5]
GTGCTGCCTTTCCTGAGTCAAAAGGCCCAGAGGGTCCTGGGGATCGACATCAGCACCACGGCGGTCAAGCTGCTGGAGCT
GTCTAAGAGCGGGGATCGGTATCGGGTGGACAGTTACGGCGTCGAGCCGTTACCGCCCAATGCTGTGGTCGAGAAGACCG
TCAATGACGAACCCAGTGTCGGCGAGACCATCGCGCGGCTGATTTCCCGTGCCCGTCCGGGCTCCAAGCTGGCGGCGGTG
GCCGTGCCCGGCTCGGCGGTGATCACCAAGACCATCGAAATGGACGCCTCTCTCGACGAAGACGAGATGGAGAACCAGCT
CAAGGTCGAGGCGGACCAGTACATCCCCTTCCCGCTGGACGAAGTGCGCATGGATTTCTGCGTCATGGGCCAGGTGGAAG
GTAACGCCGACAAGGTCGAGGTGTTGCTGGTGGCTTCGCGCACCGAGAACGTGCAGCGCCGGGTCGACTGTATCGAAATC
GGCGGCCTGAAGGCCGGCGGCGTGGACGTGGAGGCCTACGCGATGGAGCGGGCCTTTCAGCTGGTGCTGCCGCAACTGCC
GCACGGCGACGAACTGGAAACCGTGGCCGTGTTCGACATCGGTGCCACCATGACGACCCTGAATGTGCTGCATCAGGGTC
GTACTGTTTATACGCGTGAGCAGCTGTTCGGGGGCAAGCAGCTCACCGAGGAAATCCAGCGCCGCTACGGCATCTCGATG
GAAGAGGCCGGCCTGGCGAAGAAGACCGGCGAGCTGCCGGACGACTACGAGAGCGAAGTGCTGCAGCCGTTCAAGGAAGC
GATCGTGCAGCAGATCAACCGTTCGCTGCAGTTCTTCTATGGCTCCACCCAGTTCAACGAAGTGGATTACATCCTGCTGG
CGGGGGGCTCGGCGTCCATTGCCGGCTTGCCGGAGCTGGTGCAGGAGAAAATCGGGGCAAGCTGCTCGGTGGCCAATCCG
TTTACGGATATGGCCCTGGCGAGCAAGGTCAGCGCCGCCAGCATCGCCAATGATGCGCCGGCACTGATGATTGCCTGCGG
CCTGGCGCTGAGGAGCTTCGAAGAATGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0B4XI61

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.955	99.718	0.538
comM	Acinetobacter nosocomialis M2	53.955	99.718	0.538
comM	Acinetobacter baylyi ADP1	51.831	100	0.518
pilM	Legionella pneumophila strain ERS1305867	46.328	99.718	0.462