Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   S7S_RS01705 Genome accession   NZ_CP004387
Coordinates   351691..353769 (+) Length   692 a.a.
NCBI ID   WP_052269181.1    Uniprot ID   A0A0B4XJ85
Organism   Isoalcanivorax pacificus W11-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346691..358769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S7S_RS01685 (S7S_01685) pilM 348781..349848 (+) 1068 WP_041025862.1 pilus assembly protein PilM Machinery gene
  S7S_RS01690 (S7S_01690) - 349845..350414 (+) 570 WP_041025863.1 PilN domain-containing protein -
  S7S_RS01695 (S7S_01695) - 350411..351094 (+) 684 WP_041025864.1 type 4a pilus biogenesis protein PilO -
  S7S_RS01700 (S7S_01700) - 351120..351644 (+) 525 WP_238582922.1 pilus assembly protein PilP -
  S7S_RS01705 (S7S_01705) pilQ 351691..353769 (+) 2079 WP_052269181.1 type IV pilus secretin PilQ Machinery gene
  S7S_RS01710 (S7S_01710) aroK 353779..354306 (+) 528 WP_041025867.1 shikimate kinase AroK -
  S7S_RS01715 (S7S_01715) aroB 354370..355452 (+) 1083 WP_041025868.1 3-dehydroquinate synthase -
  S7S_RS01720 (S7S_01720) - 355440..356786 (+) 1347 WP_041025869.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75092.71 Da        Isoelectric Point: 4.4794

>NTDB_id=112315 S7S_RS01705 WP_052269181.1 351691..353769(+) (pilQ) [Isoalcanivorax pacificus W11-5]
MRKWVGALLVAVAGTLAGQAALASTLQSLNYASLPGNKLEVRLGFDQSPPDVRSYSIEKPARIALDLVGATNGLEGRNHN
LGTGNARSVTVVEAKDRTRLIVNLAQLTGYDTRVEGNELVLTLGVDSQTANVFEPGVDEQQAPAGRGNQVNQIDFRRGET
GEGRIVINLSNPSIPVDVREERGRIQVRFVGAAIPEALRRRLDVTDFATPVRYVDASMDGETGVVSIEPTGNWEYLAYQA
DGEFSINVKPVTEAEESRRRRDAFEYTGEKLSLNFQDIEVRSVLQLIADFTSMNLVASDTVSGRITLRLQNVPWDQALAL
ILKTKGLDKRQVGNVLLVAPADEIAAREQLELESQAQIESLAPLRTEYVRVNYARAMDLQSLIKGEGSLLSSRGSVTVDE
RTNTLIIQDTARKLEDIHEVINRLDVAVQQVLIEARVVVATSDLTDELGIRWGGLAFQGDKLAEDGRSMVFSGGATNILN
FGVGVLNGDDIEVESPDDMVVDLGSSDSRATRFSLGFVDINSGILELELSALAAEGQGEVVATPKVLTADQQPALIAAGT
QIGYQEASSSGATAVSFVNAELRLEATPRITPDGRIIMDLKINNDSVGSVIAGIPSINTNRVETTVLVNDGETVVLGGIF
QQEKREGVEKTPFLGDLPWIGRLFRREFKSDDKQELLIFITPRLLKDTYAGK

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=112315 S7S_RS01705 WP_052269181.1 351691..353769(+) (pilQ) [Isoalcanivorax pacificus W11-5]
ATGCGGAAATGGGTCGGGGCTCTGCTGGTCGCTGTGGCGGGTACGCTGGCGGGTCAGGCGGCCCTGGCATCCACGCTGCA
GTCGCTCAATTATGCGTCGCTGCCCGGCAACAAGCTGGAAGTGCGGCTGGGATTTGACCAGTCACCGCCAGATGTTCGCA
GCTACAGTATCGAGAAGCCGGCCCGTATTGCCCTGGACCTGGTGGGGGCCACCAATGGTCTGGAGGGCCGCAACCACAAT
CTGGGCACGGGAAATGCCCGCAGCGTCACGGTGGTGGAAGCGAAAGACCGGACGCGGCTGATCGTCAACCTGGCGCAACT
GACCGGTTATGACACGCGCGTGGAAGGCAATGAGCTGGTGCTCACGCTGGGTGTGGACAGCCAGACAGCGAATGTCTTCG
AACCGGGCGTCGATGAGCAACAGGCCCCGGCAGGCCGTGGCAACCAGGTCAACCAGATTGATTTCCGCCGGGGTGAAACG
GGCGAAGGCCGTATTGTCATCAACCTGTCCAACCCGTCGATTCCGGTGGACGTGCGCGAAGAGCGCGGCCGCATCCAGGT
GCGCTTCGTGGGCGCGGCGATTCCTGAAGCGTTGCGCCGCCGTCTGGACGTGACCGATTTCGCCACTCCGGTGCGCTATG
TGGATGCCAGCATGGACGGCGAGACCGGCGTGGTGTCCATCGAGCCGACCGGCAACTGGGAATACCTCGCGTATCAGGCC
GACGGCGAATTCAGTATCAATGTGAAGCCGGTGACGGAAGCGGAAGAGTCGCGCCGTCGCCGCGATGCGTTCGAATACAC
GGGTGAGAAGCTGTCGCTGAATTTCCAGGACATTGAAGTGCGTTCGGTGCTGCAATTGATTGCTGATTTTACCAGCATGA
ACCTGGTCGCGTCGGACACGGTGTCCGGCCGCATTACCCTGCGCCTGCAGAACGTACCCTGGGACCAGGCGTTGGCCCTG
ATTCTTAAAACCAAAGGGCTGGATAAGCGCCAGGTCGGTAATGTGCTGCTGGTGGCGCCGGCGGACGAGATTGCTGCGCG
TGAGCAACTGGAGCTGGAAAGTCAGGCACAGATTGAATCGCTGGCACCATTGCGTACGGAGTATGTGCGGGTCAATTACG
CACGCGCCATGGATCTGCAGTCACTGATCAAGGGAGAGGGCTCCCTGTTGTCCAGCCGTGGCTCGGTGACGGTGGACGAG
CGCACGAACACCCTGATCATCCAGGATACGGCACGCAAGCTGGAGGATATCCACGAAGTGATTAACAGACTGGATGTGGC
AGTCCAGCAGGTGTTGATTGAAGCGCGCGTGGTCGTGGCGACTTCTGATTTGACCGACGAGTTGGGTATTCGCTGGGGCG
GGCTGGCGTTCCAGGGAGACAAGCTGGCAGAAGACGGTCGTTCCATGGTGTTCAGCGGTGGCGCCACCAATATTCTTAAC
TTTGGTGTTGGTGTGCTGAATGGCGATGACATTGAGGTCGAGTCGCCGGACGACATGGTGGTGGATCTCGGCTCCTCAGA
CAGTCGTGCAACCCGTTTTTCACTTGGCTTTGTTGATATCAATTCGGGAATACTTGAGCTTGAGCTGTCTGCGCTGGCTG
CAGAAGGGCAAGGTGAAGTGGTGGCCACGCCCAAGGTGCTGACAGCGGACCAGCAGCCCGCCCTGATTGCTGCCGGTACC
CAGATCGGTTATCAGGAAGCCTCGTCCAGCGGTGCAACGGCGGTGTCCTTTGTGAACGCCGAACTTCGTCTGGAAGCGAC
ACCACGCATCACACCCGATGGCCGCATCATCATGGACCTGAAGATCAACAATGACTCCGTGGGCAGCGTCATCGCCGGCA
TTCCCAGCATCAATACCAACCGTGTAGAGACCACGGTGCTGGTGAATGATGGCGAAACCGTGGTGCTTGGTGGTATTTTC
CAGCAGGAGAAACGGGAAGGCGTGGAGAAGACCCCGTTCCTGGGTGATCTGCCGTGGATTGGCCGCCTGTTCAGACGCGA
ATTCAAGAGCGATGACAAGCAGGAACTGCTGATCTTCATTACCCCGCGCCTGCTGAAAGACACCTACGCGGGTAAATAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(294-340)

Bacterial type II/III secretion system short domain (Secretin_N)

(367-430)

AMIN domain (AMIN)

(39-123)

Bacterial type II and III secretion system protein (Secretin)

(531-685)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B4XJ85

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

47.989

100

0.5
  pilQ Acinetobacter baumannii D1279779

42.562

100

0.447
  pilQ Acinetobacter baumannii strain A118

42.562

100

0.447
  comQ Acinetobacter baylyi ADP1

42.957

100

0.445
  pilQ Legionella pneumophila strain ERS1305867

38.71

98.555

0.382
  pilQ Legionella pneumophila strain Lp02

38.563

98.555

0.38

Multiple sequence alignment