Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL203_RS07035 Genome accession   NZ_LR134383
Coordinates   1584637..1586289 (+) Length   550 a.a.
NCBI ID   WP_058470991.1    Uniprot ID   A0A0W0VAS7
Organism   Legionella jordanis strain NCTC11533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1579637..1591289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL203_RS07010 (NCTC11533_01446) mgtA 1579738..1582311 (-) 2574 WP_082647161.1 magnesium-translocating P-type ATPase -
  EL203_RS07015 (NCTC11533_01447) - 1582402..1582962 (-) 561 WP_058470994.1 hypoxanthine-guanine phosphoribosyltransferase -
  EL203_RS07020 (NCTC11533_01448) - 1582984..1583310 (-) 327 WP_058472202.1 DUF1820 family protein -
  EL203_RS07025 (NCTC11533_01449) - 1583411..1584166 (-) 756 WP_058470993.1 methyltransferase domain-containing protein -
  EL203_RS07030 (NCTC11533_01450) - 1584260..1584508 (-) 249 WP_058470992.1 hypothetical protein -
  EL203_RS07035 (NCTC11533_01451) pilB 1584637..1586289 (+) 1653 WP_058470991.1 GspE/PulE family protein Machinery gene
  EL203_RS07040 (NCTC11533_01452) - 1586286..1587488 (+) 1203 WP_058470990.1 type II secretion system F family protein -
  EL203_RS07045 (NCTC11533_01453) - 1587490..1588353 (+) 864 WP_058470989.1 A24 family peptidase -
  EL203_RS07050 (NCTC11533_01454) - 1588614..1588925 (+) 312 WP_058470988.1 hypothetical protein -
  EL203_RS07055 (NCTC11533_01455) - 1589261..1589851 (-) 591 WP_058470987.1 hypothetical protein -
  EL203_RS07060 (NCTC11533_01456) - 1589861..1590319 (-) 459 WP_058470986.1 hypothetical protein -

Sequence

Protein


Download         Length: 550 a.a.        Molecular weight: 62103.49 Da        Isoelectric Point: 6.2905

>NTDB_id=1122557 EL203_RS07035 WP_058470991.1 1584637..1586289(+) (pilB) [Legionella jordanis strain NCTC11533]
MEYNKQEQDFESIAHLFIQCNLLTEDELIHYQCDAPTSGCTLLQYLINTKSVSAKQLALLISEHFGLTYIDLDEIQLKDL
SEQFIDFELMVQHRLLPIAHKSHLILASDSPVSPSLLSELRFTSCHDFIPVIVETDKLDKWLKHQILKRNNHLEIQNKLN
EFGLNQDASVIQFLDQLLIDAVRKKASDIHFELYEEILRIRCRYDGILVEVGKYTRDLAQKLLGRIKILANLDISEKRVP
QDGRFQLEYEGNEETVDFRISICPTIHGEKMVIRILDQKTPKIPVEELGFNAVQKQNFLEAIQKPQGMILVTGPTGSGKT
VTLYSALHQLNCEDKNISTAEDPVELKMYGINQVQVHAKAGLTFASILRALLRQDPDIIMIGEIRDFETAEIAMKAAQTG
HLVFSTLHTNSAAESLTRLNTLGISNFNIANSVSLIVAQRLARRLCDHCKVSYRALSEWPQNIGKHLQTAIYRAVGCNQC
NQGYHGRIGLFEVMPLSASIAELMVSGGTSVEIHKQAQKEGMITLFESGLEKIKAGLTSFEEIRRVAVQQ

Nucleotide


Download         Length: 1653 bp        

>NTDB_id=1122557 EL203_RS07035 WP_058470991.1 1584637..1586289(+) (pilB) [Legionella jordanis strain NCTC11533]
ATGGAATACAATAAACAAGAACAAGATTTTGAGTCCATTGCTCACCTCTTCATTCAATGTAATCTGTTGACTGAGGATGA
ACTTATCCATTATCAATGTGATGCCCCAACATCGGGCTGCACACTCTTACAGTATTTAATTAATACCAAGTCCGTTTCCG
CAAAACAGTTGGCTTTATTAATATCAGAACATTTCGGCTTGACTTATATAGATCTGGATGAAATTCAACTGAAAGACCTA
TCAGAGCAATTCATTGATTTTGAGTTGATGGTTCAACATCGATTGCTGCCTATTGCCCATAAATCACATCTTATTCTGGC
AAGTGACAGCCCGGTTAGCCCCTCACTTCTTAGTGAACTTCGATTTACAAGTTGTCATGATTTTATTCCAGTGATCGTGG
AAACGGATAAATTAGATAAATGGCTCAAACACCAGATCTTAAAAAGAAATAATCACTTAGAAATTCAAAATAAATTGAAT
GAGTTTGGTTTGAACCAGGATGCATCAGTGATTCAGTTTCTAGATCAGCTTCTTATCGATGCTGTCAGAAAAAAAGCTTC
CGATATACATTTTGAACTTTATGAGGAAATTTTAAGAATTCGTTGCCGCTATGACGGCATTTTAGTTGAAGTTGGGAAAT
ATACTCGGGATTTAGCGCAAAAATTGCTCGGGCGTATCAAAATCCTTGCTAATCTTGATATTTCTGAAAAGCGCGTCCCC
CAAGATGGCCGCTTTCAGCTAGAGTATGAAGGCAATGAAGAAACGGTTGATTTTCGAATTAGCATCTGCCCTACCATCCA
CGGGGAGAAAATGGTAATTCGTATTCTTGATCAAAAAACTCCAAAGATCCCTGTTGAAGAATTGGGGTTTAACGCAGTGC
AAAAACAAAATTTTCTAGAGGCAATTCAAAAACCACAAGGAATGATTTTAGTCACAGGGCCAACTGGAAGCGGTAAAACC
GTAACGCTTTACAGCGCCTTACATCAATTAAATTGCGAAGATAAAAACATCTCTACGGCTGAGGATCCAGTCGAATTAAA
AATGTATGGAATAAACCAAGTGCAAGTGCATGCCAAGGCAGGTCTGACATTCGCCAGCATTTTACGTGCCCTATTACGCC
AAGACCCAGACATCATCATGATTGGCGAAATCCGCGATTTTGAAACAGCGGAAATTGCGATGAAAGCAGCTCAAACCGGA
CATTTAGTCTTTTCAACACTGCATACCAATAGTGCTGCAGAAAGTTTGACACGTCTTAATACGCTAGGGATTTCAAACTT
CAATATCGCCAATTCGGTGAGTTTAATTGTTGCCCAGAGGCTTGCGAGGCGTCTTTGTGATCACTGCAAGGTTTCTTATC
GGGCTCTTTCTGAATGGCCCCAAAATATCGGTAAACACCTGCAAACTGCTATTTATCGTGCAGTCGGCTGCAACCAGTGT
AATCAAGGATACCATGGCCGCATTGGCTTATTTGAAGTAATGCCCCTATCTGCATCGATTGCCGAACTCATGGTATCAGG
AGGAACCTCAGTAGAGATCCATAAGCAAGCTCAAAAGGAAGGCATGATAACTCTGTTTGAGTCAGGACTGGAAAAAATAA
AGGCTGGATTAACAAGCTTTGAAGAAATAAGACGAGTAGCGGTGCAACAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(177-444)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0VAS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

46.69

100

0.487
  pilB Vibrio cholerae strain A1552

44.746

100

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.403

96.909

0.44
  pilF Neisseria gonorrhoeae MS11

43.345

100

0.438
  pilB Acinetobacter baylyi ADP1

44.802

96.182

0.431
  pilB Vibrio campbellii strain DS40M4

44.278

96.909

0.429
  pilB Acinetobacter baumannii D1279779

44.275

95.273

0.422
  pilF Thermus thermophilus HB27

37.079

97.091

0.36

Multiple sequence alignment