Detailed information NTG1121150

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: EL107_RS07165
• Genome accession: NZ_LR134314
• Coordinates: 1422869..1423303 (-)
• Length: 144 a.a.
• NCBI ID: WP_002986542.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC8302
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1417869..1428303

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL107_RS07140 (NCTC8302_01414)	proC	1418905..1419675 (+)	771	WP_011184092.1	pyrroline-5-carboxylate reductase	-
EL107_RS07145	-	1419758..1420066 (-)	309	WP_011017251.1	hypothetical protein	-
EL107_RS07150 (NCTC8302_01415)	-	1420253..1421449 (-)	1197	WP_002986533.1	acetate kinase	-
EL107_RS07155 (NCTC8302_01416)	comYH	1421508..1422461 (-)	954	WP_021340467.1	class I SAM-dependent methyltransferase	Machinery gene
EL107_RS07160 (NCTC8302_01417)	comGG	1422559..1422885 (-)	327	WP_002986539.1	competence type IV pilus minor pilin ComGG	-
EL107_RS07165 (NCTC8302_01418)	comYF	1422869..1423303 (-)	435	WP_002986542.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL107_RS07170 (NCTC8302_01419)	comGE	1423296..1423580 (-)	285	WP_027968839.1	competence type IV pilus minor pilin ComGE	-
EL107_RS07175 (NCTC8302_01420)	comGD	1423537..1423965 (-)	429	WP_011184089.1	competence type IV pilus minor pilin ComGD	-
EL107_RS07180 (NCTC8302_01421)	comYC	1423940..1424266 (-)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
EL107_RS07185 (NCTC8302_01422)	comYB	1424268..1425302 (-)	1035	WP_011184088.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL107_RS07190 (NCTC8302_01423)	comYA	1425238..1426176 (-)	939	WP_002986557.1	competence type IV pilus ATPase ComGA	Machinery gene
EL107_RS07195 (NCTC8302_01424)	-	1426269..1426634 (-)	366	WP_002986560.1	DUF1033 family protein	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16573.20 Da
• Isoelectric Point: 10.3584

>NTDB_id=1121150 EL107_RS07165 WP_002986542.1 1422869..1423303(-) (comYF) [Streptococcus pyogenes strain NCTC8302]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1121150 EL107_RS07165 WP_002986542.1 1422869..1423303(-) (comYF) [Streptococcus pyogenes strain NCTC8302]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCATTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396