Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EL121_RS01525 Genome accession   NZ_LR134310
Coordinates   338347..338784 (-) Length   145 a.a.
NCBI ID   WP_039197145.1    Uniprot ID   A0AAX3FHE9
Organism   Actinobacillus equuli strain NCTC8529     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333347..343784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL121_RS01500 (NCTC8529_00302) yacG 334253..334441 (-) 189 WP_005623139.1 DNA gyrase inhibitor YacG -
  EL121_RS01505 (NCTC8529_00303) coaE 334423..335061 (-) 639 WP_039197141.1 dephospho-CoA kinase -
  EL121_RS01510 (NCTC8529_00304) - 335083..335757 (-) 675 WP_039197142.1 fimbrial protein -
  EL121_RS01515 (NCTC8529_00305) pilC 335757..336950 (-) 1194 WP_039197143.1 type II secretion system F family protein Machinery gene
  EL121_RS01520 - 336943..338326 (-) 1384 Protein_296 GspE/PulE family protein -
  EL121_RS01525 (NCTC8529_00308) pilA 338347..338784 (-) 438 WP_039197145.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EL121_RS01530 (NCTC8529_00309) radA 338965..340344 (+) 1380 WP_039197146.1 DNA repair protein RadA Machinery gene
  EL121_RS01535 (NCTC8529_00310) pepB 340410..341693 (+) 1284 WP_039199020.1 aminopeptidase PepB -
  EL121_RS01540 (NCTC8529_00311) - 341821..342756 (+) 936 WP_039197147.1 SIS domain-containing protein -
  EL121_RS01545 (NCTC8529_00312) greB 342766..343254 (+) 489 WP_005611555.1 transcription elongation factor GreB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15385.67 Da        Isoelectric Point: 8.0828

>NTDB_id=1120997 EL121_RS01525 WP_039197145.1 338347..338784(-) (pilA) [Actinobacillus equuli strain NCTC8529]
MKFVFKPIQQAFTLIELMIVIAIIAILATVAIPSYNNYTKKAALSELLAASSAYKTDVEICIYNTGDTKGCSNGTNGIKN
GDDLKNSKYLGTVSVVAGAITVNGKGSVAGYGYTLTPDYANKTLTWKTTCSGEDKSLFPAAFCSE

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1120997 EL121_RS01525 WP_039197145.1 338347..338784(-) (pilA) [Actinobacillus equuli strain NCTC8529]
ATGAAATTCGTATTCAAGCCTATACAGCAGGCATTTACCCTGATTGAATTAATGATTGTAATTGCAATCATTGCTATTTT
GGCAACGGTAGCCATTCCTTCTTATAACAATTACACCAAAAAGGCGGCATTATCCGAACTGTTAGCGGCATCTTCCGCTT
ATAAAACAGATGTGGAAATTTGTATTTATAACACTGGAGATACCAAAGGCTGTTCTAATGGAACAAATGGTATTAAGAAT
GGTGATGATCTTAAAAATTCTAAGTATTTGGGGACTGTCTCTGTAGTTGCTGGCGCTATAACTGTGAATGGCAAGGGCAG
TGTAGCGGGATATGGCTATACGCTTACACCTGATTATGCAAATAAAACACTGACATGGAAAACGACCTGTTCAGGTGAGG
ATAAAAGTTTGTTCCCCGCAGCATTTTGCTCGGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

62.069

100

0.621
  pilA Haemophilus influenzae 86-028NP

56.429

96.552

0.545
  pilA Haemophilus influenzae Rd KW20

56.028

97.241

0.545
  comP Acinetobacter baylyi ADP1

42.553

97.241

0.414

Multiple sequence alignment