Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   EL092_RS09570 Genome accession   NZ_LR134291
Coordinates   1989191..1989628 (-) Length   145 a.a.
NCBI ID   WP_012130937.1    Uniprot ID   A8AZH5
Organism   Streptococcus gordonii strain NCTC7868     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1984191..1994628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL092_RS09545 (NCTC7868_01908) folP 1984799..1985764 (-) 966 WP_012130932.1 dihydropteroate synthase -
  EL092_RS09550 (NCTC7868_01909) - 1985842..1986507 (-) 666 WP_012130933.1 CPBP family intramembrane glutamic endopeptidase -
  EL092_RS09555 (NCTC7868_01910) - 1986555..1987745 (-) 1191 WP_012130934.1 acetate kinase -
  EL092_RS09560 (NCTC7868_01911) comYH 1987795..1988748 (-) 954 WP_012130935.1 class I SAM-dependent methyltransferase Machinery gene
  EL092_RS09565 (NCTC7868_01912) comGG 1988779..1989210 (-) 432 WP_223341947.1 competence type IV pilus minor pilin ComGG -
  EL092_RS09570 (NCTC7868_01913) comGF/cglF 1989191..1989628 (-) 438 WP_012130937.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL092_RS09575 (NCTC7868_01914) comGE/cglE 1989612..1989905 (-) 294 WP_012130938.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL092_RS09580 (NCTC7868_01915) comYD 1989877..1990305 (-) 429 WP_012130939.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL092_RS09585 (NCTC7868_01916) comYC 1990265..1990582 (-) 318 WP_012130940.1 competence type IV pilus major pilin ComGC Machinery gene
  EL092_RS09590 (NCTC7868_01917) comYB 1990579..1991613 (-) 1035 WP_012130941.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL092_RS09595 (NCTC7868_01918) comYA 1991525..1992484 (-) 960 WP_012130942.1 competence type IV pilus ATPase ComGA Machinery gene
  EL092_RS09600 (NCTC7868_01919) - 1992586..1992954 (-) 369 WP_012130943.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16606.13 Da        Isoelectric Point: 6.8839

>NTDB_id=1120389 EL092_RS09570 WP_012130937.1 1989191..1989628(-) (comGF/cglF) [Streptococcus gordonii strain NCTC7868]
MLKKGKLAAFTLLEALVALLVISGGLLVFQAMTKLLASEIQYQEQTKAKEWLLFADQLDAELSRSHFEKVEQNKLYVKQD
GKSIAFGKSKSDDFRKTDANGRGYQPMIYGLEAAQIHKEGQIVHLLLQFRDGLEREYIYRVEEEH

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1120389 EL092_RS09570 WP_012130937.1 1989191..1989628(-) (comGF/cglF) [Streptococcus gordonii strain NCTC7868]
ATGTTGAAAAAAGGTAAACTAGCAGCCTTCACCCTGCTGGAAGCCTTAGTGGCCCTCTTGGTCATCAGTGGTGGCCTCTT
GGTTTTCCAGGCCATGACCAAGCTTCTAGCCAGCGAGATTCAGTATCAAGAGCAAACTAAGGCCAAGGAATGGCTTTTGT
TTGCGGACCAGTTAGATGCAGAATTATCTCGGAGCCATTTTGAAAAAGTCGAGCAAAATAAGCTTTATGTCAAACAAGAT
GGCAAGTCGATTGCATTTGGTAAGTCTAAAAGCGATGATTTTAGAAAAACAGATGCGAATGGCCGTGGTTATCAGCCCAT
GATTTATGGACTAGAAGCAGCTCAAATACATAAAGAAGGTCAGATAGTTCATTTGCTTCTGCAGTTTAGAGATGGCCTAG
AAAGAGAGTACATCTATCGTGTTGAAGAAGAACATTAG

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(38-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8AZH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis NCTC 12261

68.085

97.241

0.662
  comGF/cglF Streptococcus pneumoniae Rx1

66.901

97.931

0.655
  comGF/cglF Streptococcus pneumoniae D39

66.901

97.931

0.655
  comGF/cglF Streptococcus pneumoniae R6

66.901

97.931

0.655
  comGF/cglF Streptococcus pneumoniae TIGR4

66.901

97.931

0.655
  comGF/cglF Streptococcus mitis SK321

65.957

97.241

0.641
  comYF Streptococcus mutans UA140

47.887

97.931

0.469
  comYF Streptococcus mutans UA159

47.183

97.931

0.462
  comGF Lactococcus lactis subsp. cremoris KW2

42.754

95.172

0.407

Multiple sequence alignment