Detailed information NTG1120263

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: EL087_RS04085
• Genome accession: NZ_LR134288
• Coordinates: 826179..828407 (+)
• Length: 742 a.a.
• NCBI ID: WP_126406984.1
• Uniprot ID: A0A4U9YVU5
• Organism: Streptococcus milleri strain NCTC11169
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 821179..833407

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL087_RS04060	rpmG	822033..822182 (+)	150	WP_003024746.1	50S ribosomal protein L33	-
EL087_RS04065 (NCTC11169_00807)	secG	822222..822455 (+)	234	WP_003024744.1	preprotein translocase subunit SecG	-
EL087_RS04070 (NCTC11169_00808)	rnr	822547..824880 (+)	2334	WP_126406980.1	ribonuclease R	-
EL087_RS04075 (NCTC11169_00809)	smpB	824843..825310 (+)	468	WP_022526922.1	SsrA-binding protein SmpB	-
EL087_RS04080 (NCTC11169_00810)	comEA/celA/cilE	825491..826195 (+)	705	WP_126406982.1	helix-hairpin-helix domain-containing protein	Machinery gene
EL087_RS04085 (NCTC11169_00811)	comEC/celB	826179..828407 (+)	2229	WP_126406984.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EL087_RS04090 (NCTC11169_00812)	-	828488..829174 (+)	687	WP_232008265.1	DUF805 domain-containing protein	-
EL087_RS04095 (NCTC11169_00814)	-	829708..830694 (+)	987	WP_126406988.1	Gfo/Idh/MocA family protein	-
EL087_RS04100 (NCTC11169_00815)	-	830716..831369 (+)	654	WP_126406990.1	uracil-DNA glycosylase	-
EL087_RS04105 (NCTC11169_00816)	-	831406..831876 (+)	471	WP_003029850.1	NUDIX hydrolase	-
EL087_RS04110 (NCTC11169_00817)	-	831889..833166 (+)	1278	WP_126406992.1	dihydroorotase	-

Sequence

Protein

• Length: 742 a.a.
• Molecular weight: 85079.97 Da
• Isoelectric Point: 9.9008

>NTDB_id=1120263 EL087_RS04085 WP_126406984.1 826179..828407(+) (comEC/celB) [Streptococcus milleri strain NCTC11169]
MSQWIKRFPIKPIYIAFLLVWLYFAIYQSSWLGWLGFIFLVICLFRFYSPKECFMTFMILICFAGFFFIRREMVEQKTKV
EPSPIRQVAVLPDTIKVNGDSLSFRGKANGQTYQVYYKLKSKEEQLAFQNLSSLVTLTVEGEFEIPEKKRNFAGFDYQSY
LKTQGIYRILKVDTILSSQDRISLHPFEWLSSWRRKALVFIKNHFPNPMSNYMTGLLFGALDTDFDEMNNLYSSLGIIHL
FALSGMQVGLFMEGFRKLLLRLGLTQEMVHKCQYPFSFFYAGMTGFSVSVVRSLVQKLLSQHGITKLDNFALTMMILSLI
MPSFLLTAGGVLSCAYAFVISVIDFESLTSWRKIVVESSVISLGVLPILIFYFGEFQPWSILLTFVFSLIFDTMMLPGLT
LIFLSSPLIKLTQVNFLFEGLENSIRWIASVFGRPIVFGQPSPLLLIVMLLVLAILYDIRKNKKWVIFLSLLLSLLFFIN
KFPLQNEITMVDVGQGDSIFLRDWKGRNVLIDVGGREEIRTKEAWQKRATSSNAEKTLIPYLKSRGIDTIDALVLTNPNP
DYAGDVLEVAKKFAIKKIYISRSSLSNADFLEKLRKINTFIHVVKQGDKLPIFDYHLQVLSGASKNDHSIVLYGQFFRTR
FLFASDLKEEGEAKLMQHYPKLKTDVLKVGQHGAKDSSSSKFLQQIEPTVALISVGKNNQSKQPSQDIIERFAQLPAKVY
RTDEQGAVKFSGWTNWRLEMVK

Nucleotide

• Length: 2229 bp

>NTDB_id=1120263 EL087_RS04085 WP_126406984.1 826179..828407(+) (comEC/celB) [Streptococcus milleri strain NCTC11169]
ATGTCACAGTGGATTAAAAGATTTCCGATTAAGCCGATTTACATTGCTTTTTTGCTCGTCTGGTTGTATTTTGCAATCTA
TCAAAGTAGCTGGTTAGGCTGGTTGGGTTTTATCTTTCTGGTGATTTGTCTTTTTCGCTTTTATTCACCGAAAGAATGTT
TCATGACCTTCATGATTCTAATTTGTTTTGCTGGTTTTTTCTTTATTCGCAGGGAAATGGTAGAGCAGAAGACGAAAGTA
GAACCTTCTCCCATAAGACAAGTGGCAGTTCTACCTGACACGATTAAGGTAAATGGTGATTCGCTTTCTTTTCGTGGTAA
AGCTAATGGGCAGACTTATCAAGTTTACTACAAATTGAAATCAAAGGAAGAACAGTTGGCTTTTCAAAATCTCTCTAGTC
TGGTTACATTGACTGTTGAAGGGGAATTTGAAATCCCTGAGAAGAAGCGTAATTTTGCTGGTTTTGATTACCAATCCTAT
TTAAAAACGCAAGGGATTTATCGAATTTTAAAAGTGGATACCATTTTATCGAGCCAAGATAGAATCAGCTTGCACCCTTT
TGAGTGGCTTTCTAGCTGGCGAAGAAAAGCACTGGTATTTATCAAGAACCATTTTCCAAATCCGATGAGTAATTACATGA
CAGGACTCTTATTTGGTGCCTTGGATACAGACTTCGATGAAATGAACAATCTTTACTCTAGCTTGGGGATTATTCATTTA
TTTGCGCTGTCTGGCATGCAAGTTGGTTTATTTATGGAGGGTTTTCGTAAGTTGCTGTTAAGGCTGGGGCTTACACAGGA
AATGGTCCATAAATGCCAATATCCATTTTCTTTCTTCTATGCGGGAATGACTGGATTTTCAGTATCCGTTGTACGGAGCT
TAGTTCAGAAATTACTGTCGCAACATGGTATCACTAAGTTAGATAATTTTGCTTTAACGATGATGATATTGTCCTTGATT
ATGCCTTCCTTTCTTTTAACGGCCGGAGGAGTTCTCTCTTGCGCCTATGCTTTTGTTATTAGTGTGATAGATTTTGAAAG
TCTGACTTCTTGGCGAAAGATTGTTGTAGAGAGCAGTGTCATTTCGCTTGGTGTTTTACCGATTCTAATCTTTTATTTTG
GTGAATTTCAACCTTGGTCTATTTTGTTGACATTTGTTTTTTCACTGATTTTCGATACAATGATGCTGCCAGGCTTGACG
TTGATTTTTCTTTCTTCGCCTTTGATAAAGCTAACTCAGGTCAATTTTCTATTTGAAGGGTTAGAAAATAGCATTCGTTG
GATAGCAAGTGTCTTTGGCAGACCAATCGTTTTTGGGCAACCCAGTCCGCTTTTGCTGATTGTTATGCTACTTGTACTGG
CTATTTTGTATGATATTAGAAAAAATAAAAAATGGGTAATATTTCTTAGTCTGCTTCTTTCATTACTCTTTTTCATAAAT
AAATTTCCCTTGCAAAATGAGATCACAATGGTTGATGTTGGGCAGGGAGATAGTATTTTTCTGAGAGACTGGAAAGGACG
AAATGTATTGATTGATGTTGGGGGACGTGAGGAAATTAGAACAAAAGAAGCTTGGCAAAAACGAGCGACTAGCTCAAATG
CAGAGAAAACTTTGATTCCTTACTTAAAAAGTCGTGGCATAGATACGATTGATGCTTTAGTCTTAACAAATCCTAATCCA
GATTATGCAGGAGATGTATTAGAAGTTGCTAAAAAATTTGCAATAAAGAAAATTTATATTTCCAGAAGTAGTCTAAGCAA
TGCAGATTTTCTAGAGAAATTAAGGAAAATAAACACATTCATTCATGTTGTAAAACAAGGCGACAAACTTCCTATTTTTG
ATTATCATTTGCAAGTTCTTTCGGGTGCTAGCAAGAACGACCATTCGATCGTTTTATATGGTCAGTTTTTCCGTACAAGA
TTTTTATTTGCGAGCGACTTGAAAGAAGAGGGCGAAGCAAAGTTAATGCAGCATTATCCAAAGTTGAAAACAGATGTTTT
GAAAGTCGGACAGCATGGAGCCAAGGACTCATCAAGTTCAAAATTTTTACAACAAATAGAACCAACGGTCGCTCTCATTT
CTGTTGGGAAAAATAATCAATCTAAGCAACCAAGTCAAGATATAATAGAGCGATTTGCACAATTGCCTGCTAAGGTCTAT
CGGACAGATGAACAAGGGGCAGTTAAATTTTCAGGGTGGACAAACTGGCGATTAGAGATGGTCAAGTAA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-592)

• Competence protein (Competence) (216-460)

• Domain of unknown function (DUF4131) (DUF4131) (29-175)

3D structure

• AlphaFold DB: A0A4U9YVU5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	55.422	100	0.558
comEC/celB	Streptococcus mitis NCTC 12261	54.692	100	0.55
comEC/celB	Streptococcus pneumoniae TIGR4	54.351	100	0.547
comEC/celB	Streptococcus pneumoniae Rx1	53.548	100	0.539
comEC/celB	Streptococcus pneumoniae D39	53.548	100	0.539
comEC/celB	Streptococcus pneumoniae R6	53.548	100	0.539
comEC	Lactococcus lactis subsp. cremoris KW2	47.177	100	0.473