Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EL064_RS04255 Genome accession   NZ_LR134178
Coordinates   973902..974966 (+) Length   354 a.a.
NCBI ID   WP_115701265.1    Uniprot ID   -
Organism   Legionella sainthelensi strain NCTC12450     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 968902..979966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL064_RS04245 (NCTC12450_00888) - 969646..972012 (-) 2367 WP_115701106.1 penicillin-binding protein 1A -
  EL064_RS04250 (NCTC12450_00889) - 972403..973665 (-) 1263 WP_241969646.1 M protein -
  EL064_RS04255 (NCTC12450_00890) pilM 973902..974966 (+) 1065 WP_115701265.1 type IV pilus assembly protein PilM Machinery gene
  EL064_RS04260 (NCTC12450_00891) pilN 974971..975534 (+) 564 WP_115701110.1 PilN domain-containing protein Machinery gene
  EL064_RS04265 (NCTC12450_00892) pilO 975531..976127 (+) 597 WP_115701113.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL064_RS04270 (NCTC12450_00893) pilP 976127..976633 (+) 507 WP_115701116.1 pilus assembly protein PilP Machinery gene
  EL064_RS04275 (NCTC12450_00894) pilQ 976642..978747 (+) 2106 WP_115701119.1 type IV pilus secretin PilQ Machinery gene
  EL064_RS04280 (NCTC12450_00895) aroK 979077..979598 (+) 522 WP_027270601.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38995.02 Da        Isoelectric Point: 4.8910

>NTDB_id=1118798 EL064_RS04255 WP_115701265.1 973902..974966(+) (pilM) [Legionella sainthelensi strain NCTC12450]
MLQLLQPQHRSTLGIDITSTAVKILEISGKNGDLVIENYGRELLPPNAMDGNVIKDLDAVSQCVKKIVDRLHTPCKQAAL
AVPDAAIISKVVQINEGLNDEEMEELIATEADKYIPYPIEEINLDFEVLGPYQKNPHLLDVLIVASRAENVNQRVGIATA
AGLEAVVVDVESYAVERAAQRIASDLPGLGKEKTIAIIDIGACYTHLFVLHKMKLVYSREEKFGDKQLVESIAERYKITL
EEATVARDKKQLPSNYETEVLEPFKENILLQIKRTLQFFYSTNPEGEVDHILLAGGLARLPGLVTLVKDRLGKAAAIANP
LSYMTPSKKIHLDDINNDAPALMVACGLALRNVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1118798 EL064_RS04255 WP_115701265.1 973902..974966(+) (pilM) [Legionella sainthelensi strain NCTC12450]
ATGCTTCAATTATTGCAACCCCAGCATCGTTCAACTCTTGGAATAGACATAACATCCACAGCAGTCAAAATATTAGAAAT
CTCAGGTAAGAATGGTGATCTTGTTATTGAGAATTACGGACGTGAACTATTGCCACCCAATGCAATGGATGGAAATGTTA
TCAAGGATCTCGATGCTGTCTCTCAATGTGTTAAAAAAATAGTTGACCGATTGCATACCCCGTGTAAGCAAGCTGCATTG
GCTGTGCCAGATGCGGCGATAATTAGTAAAGTAGTGCAAATTAATGAAGGCTTAAATGATGAGGAGATGGAGGAGCTTAT
CGCGACTGAGGCTGATAAATATATTCCTTATCCAATTGAAGAAATTAATCTTGATTTCGAGGTTTTGGGACCCTATCAGA
AAAACCCACATTTACTTGATGTGCTTATCGTTGCTTCGCGAGCGGAAAATGTCAACCAGCGTGTTGGAATAGCTACGGCT
GCGGGTTTAGAGGCTGTAGTAGTAGATGTTGAATCTTACGCAGTGGAGCGAGCAGCACAACGAATAGCTTCAGATTTACC
AGGACTTGGTAAAGAAAAGACCATTGCTATTATTGATATTGGGGCATGTTATACTCATTTATTCGTATTGCATAAAATGA
AATTAGTTTATTCTCGAGAAGAAAAATTTGGCGATAAGCAATTAGTTGAATCAATTGCTGAGCGCTATAAAATAACCTTA
GAGGAGGCAACAGTTGCCAGGGATAAAAAACAATTACCTTCTAATTATGAAACAGAAGTTTTAGAGCCTTTTAAAGAAAA
TATCTTGTTGCAGATTAAGAGGACGTTGCAGTTTTTTTATTCTACAAACCCAGAAGGAGAGGTTGATCATATTCTATTGG
CTGGGGGATTAGCAAGATTACCAGGCTTAGTTACTCTAGTTAAAGACCGATTAGGAAAAGCAGCTGCTATCGCTAATCCA
CTTTCATATATGACCCCAAGTAAAAAAATCCATTTGGACGACATAAATAATGATGCGCCCGCACTTATGGTTGCTTGTGG
TTTAGCATTAAGAAATGTAGAGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

66.102

100

0.661
  pilM Acinetobacter baumannii D1279779

43.22

100

0.432
  comM Acinetobacter nosocomialis M2

43.22

100

0.432
  comM Acinetobacter baylyi ADP1

40.678

100

0.407

Multiple sequence alignment