Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EL064_RS04260 Genome accession   NZ_LR134178
Coordinates   974971..975534 (+) Length   187 a.a.
NCBI ID   WP_115701110.1    Uniprot ID   -
Organism   Legionella sainthelensi strain NCTC12450     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 969971..980534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL064_RS04250 (NCTC12450_00889) - 972403..973665 (-) 1263 WP_241969646.1 M protein -
  EL064_RS04255 (NCTC12450_00890) pilM 973902..974966 (+) 1065 WP_115701265.1 type IV pilus assembly protein PilM Machinery gene
  EL064_RS04260 (NCTC12450_00891) pilN 974971..975534 (+) 564 WP_115701110.1 PilN domain-containing protein Machinery gene
  EL064_RS04265 (NCTC12450_00892) pilO 975531..976127 (+) 597 WP_115701113.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL064_RS04270 (NCTC12450_00893) pilP 976127..976633 (+) 507 WP_115701116.1 pilus assembly protein PilP Machinery gene
  EL064_RS04275 (NCTC12450_00894) pilQ 976642..978747 (+) 2106 WP_115701119.1 type IV pilus secretin PilQ Machinery gene
  EL064_RS04280 (NCTC12450_00895) aroK 979077..979598 (+) 522 WP_027270601.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 21850.52 Da        Isoelectric Point: 7.2778

>NTDB_id=1118799 EL064_RS04260 WP_115701110.1 974971..975534(+) (pilN) [Legionella sainthelensi strain NCTC12450]
MTQINLLPWREQKREQEKKVFMVLFCIVIAISIFVVFLINTYASDLVSNQLVRNQMLQDEIAAMNNQLNEIKSLKKTREL
FISRMFIVQHLQSTRTLMVHLFDELINVMPSGIYLTKVEGKNDILSVTGYTESNTYVSILMKNIENNEWIHSPVLSEIKK
EDRQEPANNEFKLNFVLAPQFQLGIIR

Nucleotide


Download         Length: 564 bp        

>NTDB_id=1118799 EL064_RS04260 WP_115701110.1 974971..975534(+) (pilN) [Legionella sainthelensi strain NCTC12450]
ATGACACAAATTAATCTTCTTCCTTGGCGTGAACAAAAGCGGGAGCAAGAAAAAAAGGTGTTCATGGTTTTATTTTGCAT
AGTTATAGCAATTTCAATATTTGTCGTGTTTTTAATTAATACTTATGCGTCTGACTTAGTGAGTAATCAACTTGTTCGAA
ATCAGATGCTGCAAGATGAAATAGCGGCTATGAATAACCAGCTAAACGAAATTAAAAGCTTGAAAAAAACTAGAGAGCTG
TTCATCTCTAGAATGTTTATCGTTCAGCATTTACAATCAACCAGGACATTAATGGTTCATTTATTTGATGAGTTAATTAA
TGTAATGCCTTCTGGCATTTATTTGACAAAAGTAGAAGGGAAAAATGATATTCTCTCTGTCACTGGGTATACTGAATCGA
ACACCTACGTTTCAATTTTAATGAAAAATATAGAGAATAATGAATGGATACATTCTCCTGTTCTAAGTGAAATTAAGAAG
GAAGACAGGCAGGAGCCTGCTAATAATGAATTTAAGTTAAATTTTGTACTTGCTCCTCAATTCCAGCTGGGAATTATACG
ATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

61.749

97.861

0.604

Multiple sequence alignment