Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AWM52_RS11450 Genome accession   NZ_LN854573
Coordinates   2526947..2528011 (-) Length   354 a.a.
NCBI ID   WP_027922100.1    Uniprot ID   -
Organism   Pseudomonas sp. URMO17WK12:I11 isolate Shine     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2521947..2533011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM52_RS11425 (PSHI_23560) aroK 2522640..2523158 (-) 519 WP_027922095.1 shikimate kinase AroK -
  AWM52_RS11430 (PSHI_23570) pilQ 2523163..2525229 (-) 2067 WP_059181773.1 type IV pilus secretin PilQ Machinery gene
  AWM52_RS11435 (PSHI_23580) - 2525243..2525770 (-) 528 WP_027922097.1 pilus assembly protein PilP -
  AWM52_RS11440 (PSHI_23590) pilO 2525767..2526384 (-) 618 WP_059181774.1 type 4a pilus biogenesis protein PilO -
  AWM52_RS11445 (PSHI_23600) - 2526381..2526947 (-) 567 WP_059181775.1 PilN domain-containing protein -
  AWM52_RS11450 (PSHI_23610) pilM 2526947..2528011 (-) 1065 WP_027922100.1 pilus assembly protein PilM Machinery gene
  AWM52_RS11455 (PSHI_23620) - 2528229..2530673 (+) 2445 WP_059181776.1 penicillin-binding protein 1A -
  AWM52_RS11460 (PSHI_23630) - 2530814..2532082 (-) 1269 WP_027922102.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38108.55 Da        Isoelectric Point: 4.7417

>NTDB_id=1114706 AWM52_RS11450 WP_027922100.1 2526947..2528011(-) (pilM) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
MLGLFNKKANSLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLLKARTSLKSVAV
AVAGSAVITKTIEMDGGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGVSARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLSTQLAATQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVNEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVSGLDRLIEQRLDTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1114706 AWM52_RS11450 WP_027922100.1 2526947..2528011(-) (pilM) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
GTGCTAGGACTCTTCAATAAAAAAGCCAACTCGCTGTTGGGGATCGACATCAGTTCCACGTCGGTAAAGCTTCTGGAACT
GAGTCGTCAGGGTGACCGTTATCGGGTCGAGGCGTACGCGGTAGAGCCGTTGCCGGCCAATGCGGTAGTCGAAAAAAACA
TCGCTGAGCTCGAGGGTGTCGGGCAGGCGCTCTCTCGTGTGCTGCTCAAGGCCAGAACCAGCCTCAAGAGCGTGGCGGTG
GCAGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGGTGGTCTTTCCGACGACGAGATGGAAAACCAGCT
CAAGATCGAGGCCGATCAGTACATTCCCTACCCTTTGGATGAAGTCGCCATCGACTTCGAAGTCCAGGGCGTGTCCGCGC
GTAACCCGGAGCGGGTCAATGTGTTGCTGGCCGCCTGTCGCAAGGAAAATGTCGAAGTCCGAGAGGCGGCTCTGGCCTTG
GCCGGCCTGACTGCGCGCGTGGTCGATGTCGAGGCTTACGCGCTGGAGCGTTCCTTCGGCTTGCTCTCGACACAACTGGC
CGCCACCCAGGAGCGCCTGACGGTTGCGGTGGTGGACATTGGCGCGACCATGACCACCCTCAGCGTGCTGCACAACGGCA
AGATCATTTATACCCGCGAGCAATTGTTCGGGGGGCGGCAGCTGACCGAGGAAATTCAGCGCCGGTATGGCCTGACGGTC
GAGCAGGCCGGGCTGGCGAAGAAGCAGGGCGGATTGCCAGACGACTACGTCAACGAAGTCTTGCAACCGTTTCGCGAGGC
GCTGGTACAGCAGGTTTCGCGCTCGTTGCAGTTCTTCTTTGCCTCTGGCCAGTACAACGCGGTCGATCACATTCTGCTGG
CCGGTGGCACGGCGTCGGTGTCGGGGCTGGACCGGTTGATCGAACAGCGCCTGGATACGCCGACTCAGGTGGCCAACCCG
TTCGCCAACATGGCCTTGAGCAGCAAGGTCAACGCCGGCGCGCTGGCTAGCGATGCGCCAGCCTTGATGATCGCGTGCGG
GCTCGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469

Multiple sequence alignment