Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AWM52_RS11430 Genome accession   NZ_LN854573
Coordinates   2523163..2525229 (-) Length   688 a.a.
NCBI ID   WP_059181773.1    Uniprot ID   -
Organism   Pseudomonas sp. URMO17WK12:I11 isolate Shine     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2518163..2530229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM52_RS11415 (PSHI_23540) - 2519786..2521378 (-) 1593 WP_027922093.1 AAA family ATPase -
  AWM52_RS11420 (PSHI_23550) aroB 2521389..2522489 (-) 1101 WP_059181772.1 3-dehydroquinate synthase -
  AWM52_RS11425 (PSHI_23560) aroK 2522640..2523158 (-) 519 WP_027922095.1 shikimate kinase AroK -
  AWM52_RS11430 (PSHI_23570) pilQ 2523163..2525229 (-) 2067 WP_059181773.1 type IV pilus secretin PilQ Machinery gene
  AWM52_RS11435 (PSHI_23580) - 2525243..2525770 (-) 528 WP_027922097.1 pilus assembly protein PilP -
  AWM52_RS11440 (PSHI_23590) pilO 2525767..2526384 (-) 618 WP_059181774.1 type 4a pilus biogenesis protein PilO -
  AWM52_RS11445 (PSHI_23600) - 2526381..2526947 (-) 567 WP_059181775.1 PilN domain-containing protein -
  AWM52_RS11450 (PSHI_23610) pilM 2526947..2528011 (-) 1065 WP_027922100.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 688 a.a.        Molecular weight: 74580.75 Da        Isoelectric Point: 5.4153

>NTDB_id=1114705 AWM52_RS11430 WP_059181773.1 2523163..2525229(-) (pilQ) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
MNRTFSTLGISLWIALVSPMVLASNLKALDVAALPGDRVELKLSFDGPPPVPDGYTTEQPARIALDLPGVVSQLKSKTRD
LGGGNARSATVVEANDRTRLIINLTQLTPYNARVEGNNLLVVVGQGANSKASKPAARAAVATPAPAKTYAPSSKAIRGVD
FQRGTQGEGNVVIDLSDPSIAPDIQEHEGKIILGFARTQLPEKLRVRLDVKDFATPVQFVNAVATGDRATITIEPGGAFD
YSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPW
DQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQISELAPLRRELLQVNYAKAADIAKLFTSVTSAEAKAD
ERGSITVDERTNNIIAYQPQDRLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVRNKGNWNTSGASDGR
TIGTPESTSTNSSFVDMGAAGSTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQEAS
SSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQSK
VVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2067 bp        

>NTDB_id=1114705 AWM52_RS11430 WP_059181773.1 2523163..2525229(-) (pilQ) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
ATGAACAGGACTTTCTCAACCCTCGGCATTTCGCTATGGATAGCGTTGGTGTCTCCGATGGTGCTGGCGAGCAACCTCAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGATCGCGTCGAGTTGAAGTTGTCTTTCGATGGGCCGCCACCGGTGCCCGACG
GTTACACGACCGAACAGCCGGCGCGGATTGCACTGGACCTGCCCGGCGTGGTCAGTCAGCTGAAGAGCAAGACGCGCGAC
CTCGGTGGCGGCAATGCCCGCAGTGCCACGGTGGTCGAGGCCAACGACCGCACGCGATTGATCATTAACCTGACGCAGTT
GACGCCGTATAACGCGAGGGTCGAAGGCAACAACCTGTTGGTGGTGGTCGGGCAGGGGGCCAACAGCAAGGCGTCGAAAC
CGGCGGCTCGCGCCGCGGTGGCAACACCGGCGCCGGCTAAAACCTACGCCCCTTCGAGCAAGGCCATTCGCGGCGTCGAC
TTCCAGCGGGGTACCCAGGGTGAAGGCAATGTGGTCATCGACCTGTCGGATCCGTCCATCGCACCAGACATCCAGGAACA
CGAAGGCAAGATCATCCTCGGTTTTGCCAGGACCCAATTGCCCGAAAAGTTGCGTGTGCGCCTCGACGTCAAGGATTTCG
CCACTCCGGTGCAATTCGTCAACGCCGTCGCCACTGGCGACCGTGCCACGATCACCATCGAGCCTGGCGGCGCGTTCGAC
TATTCGACCTATCAGACCGACAACAAGCTGACCGTCAGCATCCGGCCGATGACGGTCGACGATCTGCAAAAGCGCAACGC
AGAGCGCTTCGCCTACACCGGCGAAAAACTCTCGCTGAATTTCCAGGACATCGACGTGCGCTCGGTGCTGCAACTGATCG
CCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACCGTGCAGGGCGGCATCACGTTGCGGTTGCAAAACGTACCATGG
GATCAGGCACTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGATCGGTAACGTGCTGTTAGTGGCGCCGGC
CGATGAAATCGCCGCCCGGGAACGCCAGGAACTGGAATCGCAGAAGCAGATCTCCGAACTGGCGCCGCTGCGTCGCGAGC
TGTTGCAGGTCAACTACGCCAAGGCGGCGGACATTGCCAAGTTGTTCACTTCAGTGACCAGTGCCGAGGCGAAAGCCGAC
GAGCGGGGGTCGATCACCGTTGATGAGCGCACCAACAACATCATTGCTTACCAGCCCCAGGACCGTCTCGATGAGCTTCG
CCGCATCGTCACGCAACTGGACATCCCGGTTCGCCAGGTGATGATCGAAGCGCGAATCGTCGAAGCCAACGTCGATTACG
ACAAAAGCCTCGGCGTGCGCTGGGGTGGTTCGGTGCGGAACAAGGGCAACTGGAATACGTCCGGGGCCAGCGATGGGAGG
ACCATCGGCACACCGGAAAGTACCAGTACCAACTCGTCGTTTGTCGACATGGGCGCAGCAGGCAGCACCTCGGGCATCGG
CATCGCCTTCATCACCGACAACGTGTTGCTGGACCTGGAACTGACGGCGATGGAGAAAACCGGCAACGGCGAAATCGTCT
CGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCAAAAATCCTCAAGGGCACGGAGATTCCCTACCAGGAAGCCAGC
TCCAGTGGCGCGACTTCGGTGTCTTTCAAGGAAGCCTCGCTGTCGCTGGAAGTGACACCACAGATCACCCCGGACAACCG
GATTATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGCAGGATGTACCGCCGATCAAGAAAA
ACGAGGTCAACGCCAAGGTCCTGGTCAACGATGGCGAGACCATCGTGATTGGCGGGGTTTTCTCAAATACTCAAAGCAAG
GTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTCGGAGAAAAA
ATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II and III secretion system protein (Secretin)

(523-679)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.727

100

0.756
  comQ Acinetobacter baylyi ADP1

39.917

100

0.42
  pilQ Acinetobacter baumannii strain A118

37.867

100

0.413
  pilQ Acinetobacter baumannii D1279779

37.867

100

0.413
  pilQ Legionella pneumophila strain ERS1305867

37.946

97.674

0.371
  pilQ Legionella pneumophila strain Lp02

37.946

97.674

0.371

Multiple sequence alignment