Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BN1079_RS14055 Genome accession   NZ_CCSF01000001
Coordinates   3033403..3035514 (+) Length   703 a.a.
NCBI ID   WP_037025477.1    Uniprot ID   A0A078LWC0
Organism   Pseudomonas saudiphocaensis strain 20_BN     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3028403..3040514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BN1079_RS14035 (BN1079_02881) comM 3030569..3031633 (+) 1065 WP_037025465.1 pilus assembly protein PilM Machinery gene
  BN1079_RS14040 (BN1079_02882) pilN 3031633..3032205 (+) 573 WP_037025468.1 type 4a pilus biogenesis protein PilN -
  BN1079_RS14045 (BN1079_02883) pilO 3032202..3032825 (+) 624 WP_037025471.1 type 4a pilus biogenesis protein PilO -
  BN1079_RS14050 (BN1079_02884) pilP 3032822..3033349 (+) 528 WP_037025474.1 type 4a pilus biogenesis lipoprotein PilP -
  BN1079_RS14055 (BN1079_02885) pilQ 3033403..3035514 (+) 2112 WP_037025477.1 type IV pilus secretin PilQ Machinery gene
  BN1079_RS14060 (BN1079_02886) aroK 3035520..3036065 (+) 546 WP_074436845.1 shikimate kinase AroK -
  BN1079_RS14065 (BN1079_02887) aroB 3036092..3037195 (+) 1104 WP_037025480.1 3-dehydroquinate synthase -
  BN1079_RS14070 (BN1079_02888) - 3037206..3038783 (+) 1578 WP_037025482.1 AAA family ATPase -

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 75926.92 Da        Isoelectric Point: 4.7487

>NTDB_id=111355 BN1079_RS14055 WP_037025477.1 3033403..3035514(+) (pilQ) [Pseudomonas saudiphocaensis strain 20_BN]
MNTSLSRLGLSLLAALISPALLAANLNTIDVASLPGDKVELKLGFDEPVAAPRGYTLDQPARIALDLPGVSNKLGARNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGNNLFVLLGEGGTPTPVAAPAPAQPVVGTAPVERVIAGKSISNVD
FRRGEDGAGNVVITLSDPSVSPNIEEQGGKIRVSFAKTQLPEALRVRLDVQDFATPVKFVDSSSRADGATISIEPTGRYD
YLAYQTDDKLTISVKPLTESEAEQRKAEHFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRLQNVPW
DQALDLVLKTKGLDKRQVGNVLLVAPAEEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTDREGASE
DRGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGARSDGKWSIYGKDGAQT
FDEDGRRYLPGTDTFGNYTSDDGVAPVPFVDMGVLNSTSGIGIGFITDNVILDLQLSAMEKTGNGEIVSQPKVMTADKET
AKILKGSEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFGRVLNGVPPINKNEVNAKILVADG
ETVVIGGVFSNSQTKSVDKVPFLGDLPYLGRLFRRDSVQDAKSELLVFLTPKILNHQAIAVSR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=111355 BN1079_RS14055 WP_037025477.1 3033403..3035514(+) (pilQ) [Pseudomonas saudiphocaensis strain 20_BN]
ATGAATACCTCCCTCTCTCGTCTCGGTTTGTCCCTTCTGGCTGCGCTCATTTCGCCTGCGTTGCTGGCAGCGAATCTCAA
CACCATCGATGTGGCCTCCCTGCCGGGCGATAAGGTCGAGCTCAAGCTTGGGTTTGACGAGCCTGTGGCCGCTCCGCGTG
GTTACACCCTCGATCAGCCAGCACGTATCGCGCTCGATCTGCCTGGTGTGTCGAACAAGCTTGGCGCCAGGAATCGCGAG
CTGGGCGTTGGCAATGCGCGCAGCGTGACTGTTGTCGAGGCGCAGGGGCGTACCCGGCTGATCGTCAACCTCACTTCGCT
GGTGCCCTATTCCACGCGTGTGGATGGCAATAACCTGTTCGTCCTGCTGGGTGAGGGTGGCACGCCCACTCCGGTGGCTG
CGCCTGCGCCAGCACAGCCAGTGGTTGGTACTGCGCCGGTTGAGCGTGTGATTGCAGGCAAGTCCATCAGCAATGTCGAT
TTCCGGCGTGGAGAAGATGGCGCCGGTAATGTGGTCATCACTCTGTCGGATCCTTCGGTCAGCCCGAACATCGAAGAGCA
GGGCGGCAAGATTCGTGTTTCCTTTGCCAAGACACAGCTGCCGGAGGCACTGCGTGTGCGTCTTGACGTGCAGGACTTCG
CCACGCCGGTAAAATTTGTCGACTCGAGCAGTCGGGCTGATGGCGCAACCATTTCCATCGAGCCAACAGGCCGCTACGAC
TACCTTGCCTACCAGACCGACGACAAGCTCACCATCAGCGTCAAACCGCTGACCGAGTCCGAGGCCGAGCAGCGTAAGGC
CGAGCACTTTGCCTATTCGGGTGAAAAGCTGTCATTGAATTTTCAGGATATCGATGTGCGCTCGGTGCTTCAGCTGATTG
CCGACTTCACTGATCTCAACCTGGTAGCCAGTGACACAGTAAGCGGGAACATCACGCTGCGCCTGCAGAATGTGCCATGG
GACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGTCTCGACAAGCGCCAGGTTGGTAACGTCTTGCTGGTCGCGCCGGC
TGAAGAGATCGCTGCACGCGAACGGCAGGAGCTGGAATCACAGCGCCAGATCGCCGAACTGGCGCCGCTGCGCCGTGAGG
TGGTTCAGGTCAACTATGCCAAAGCGGCCGACATTGCCCGCCTGTTCCAATCGGTAACCGACCGGGAAGGCGCCAGTGAG
GACCGCGGCTCCATTGCCGTTGACGATCGCACCAACAACATCATTGCCTACCAGACCCAGGAGCGACTGGACGAGCTGCG
CCGCATCGTGGCGCAACTGGATATCCCGGTCCGTCAGGTGATGATCGAGGCGCGTATCGTCGAAGCCAACGTGGACTACG
ACAAAAGCCTGGGTGTGCGTTGGGGCGGTGCAAGGTCGGATGGGAAGTGGAGCATCTACGGCAAGGATGGAGCCCAGACC
TTTGATGAGGATGGCCGTCGTTACCTTCCGGGAACAGATACCTTCGGCAACTACACATCCGATGACGGTGTAGCGCCGGT
GCCCTTTGTGGATATGGGCGTGCTCAACAGCACCTCGGGTATCGGTATCGGTTTCATCACTGACAACGTAATCCTGGACC
TGCAACTGTCAGCCATGGAAAAAACCGGTAACGGCGAAATCGTTTCGCAGCCCAAGGTCATGACCGCTGATAAAGAGACG
GCCAAAATTCTGAAAGGCTCGGAGATCCCCTATCAGGAAGCCAGCTCCAGCGGTGCAACCAGCACTTCGTTCAAAGAGGC
GGCGCTGTCCCTGGAAGTGACCCCGCAGATCACACCTGACAACCGCATCATCATGGAAGTGAAGGTGACCAAGGATGCTC
CTGACTTCGGCCGGGTGCTCAATGGTGTGCCGCCAATCAACAAGAATGAGGTCAACGCCAAGATTCTGGTTGCCGATGGT
GAGACCGTTGTCATCGGCGGCGTCTTTTCGAACTCGCAGACCAAGAGCGTAGACAAGGTTCCTTTCCTAGGAGATCTGCC
GTATCTTGGCCGCCTTTTCCGCCGAGACTCGGTGCAAGATGCGAAGTCGGAACTCCTGGTCTTCCTTACACCGAAAATCC
TCAATCATCAGGCAATAGCAGTGAGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-122)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-439)

Bacterial type II and III secretion system protein (Secretin)

(538-694)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A078LWC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.14

100

0.765
  comQ Acinetobacter baylyi ADP1

41.345

99.431

0.411
  pilQ Acinetobacter baumannii D1279779

39.16

100

0.411
  pilQ Acinetobacter baumannii strain A118

39.16

100

0.411

Multiple sequence alignment