Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ACK8OV_RS18330 Genome accession   NZ_JBKHUK010000001
Coordinates   3740674..3742194 (-) Length   506 a.a.
NCBI ID   WP_023331589.1    Uniprot ID   A0A2J0PD61
Organism   Enterobacter kobei strain L5089hy     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 3735674..3747194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8OV_RS18320 (ACK8OV_18320) hdfR 3739366..3740187 (-) 822 WP_014885681.1 HTH-type transcriptional regulator HdfR -
  ACK8OV_RS18325 (ACK8OV_18325) - 3740306..3740644 (+) 339 WP_014885680.1 DUF413 domain-containing protein -
  ACK8OV_RS18330 (ACK8OV_18330) comM 3740674..3742194 (-) 1521 WP_023331589.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  ACK8OV_RS18335 (ACK8OV_18335) ilvL 3742536..3742634 (+) 99 WP_001311244.1 ilv operon leader peptide -
  ACK8OV_RS18340 (ACK8OV_18340) ilvX 3742721..3742771 (+) 51 WP_201405605.1 peptide IlvX -
  ACK8OV_RS18345 (ACK8OV_18345) ilvG 3742774..3744420 (+) 1647 WP_045135411.1 acetolactate synthase 2 catalytic subunit -
  ACK8OV_RS18350 (ACK8OV_18350) ilvM 3744417..3744680 (+) 264 WP_003860184.1 acetolactate synthase 2 small subunit -
  ACK8OV_RS18355 (ACK8OV_18355) ilvE 3744699..3745628 (+) 930 WP_014885677.1 branched-chain-amino-acid transaminase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55055.09 Da        Isoelectric Point: 7.3092

>NTDB_id=1113356 ACK8OV_RS18330 WP_023331589.1 3740674..3742194(-) (comM) [Enterobacter kobei strain L5089hy]
MSLSVVYTRAALGVKAPLISVEVHLSNGLPGLTLVGLPETTVKEARDRVRSAIINSGYTFPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLNTTRLGSYEFVGELALTGALRGVPGAISGALEAIRAGRQIIVANENASEVSLIAEKGCLVAGHL
QEVCAWLEGRHELSEPEECDNVIADTPEDLSEIMGQEQGKRALEITAAGGHNLLLIGPPGTGKTMLASRLSGLLPPLNNH
EALESAAIYSLISSTSLQKQWRRRPFRSPHHSASLTAMVGGGSIPGPGEISLAHNGILFLDELPEFERRVLDALREPIES
GEIHISRTRAKISYPAQFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLGKLSGPFLDRFDLSLEIPLPPPGLLRQKGITG
ESSADVRERVIAAQTRQYARQNRLNARLDNAGIRQFCSLNMEDAVWLEETLTRFGLSIRAWQRLLKVARTIADVEGCSDI
QRKHLQEALSYRAIDRLLLHLQKLLA

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=1113356 ACK8OV_RS18330 WP_023331589.1 3740674..3742194(-) (comM) [Enterobacter kobei strain L5089hy]
ATGTCACTGTCAGTTGTTTATACGCGTGCGGCTCTCGGGGTGAAGGCACCGCTTATTTCTGTAGAGGTTCATCTGAGTAA
TGGGCTACCCGGGCTCACGCTCGTCGGGCTACCTGAAACGACGGTTAAAGAAGCCCGGGATCGCGTTCGCAGCGCAATAA
TAAATAGCGGTTATACCTTCCCCGCGAAGAAGATCACCATCAACCTGGCTCCCGCCGATCTGCCCAAAGAGGGAGGACGA
TATGATTTACCTATCGCGATTGCGCTTCTCGCAGCCTCTGAGCAGCTTAATACGACCAGGCTAGGCTCGTACGAGTTTGT
GGGAGAACTCGCGCTCACAGGCGCGTTAAGGGGCGTTCCCGGTGCGATATCGGGCGCGCTGGAAGCTATACGTGCAGGAC
GGCAAATCATTGTCGCGAATGAAAATGCATCAGAAGTGAGTCTTATCGCCGAGAAGGGGTGTCTTGTCGCAGGGCATTTA
CAGGAAGTTTGCGCCTGGCTGGAAGGGCGCCATGAACTGTCCGAGCCGGAAGAGTGTGACAATGTTATAGCCGATACCCC
AGAGGATCTCAGCGAGATTATGGGACAGGAGCAAGGGAAACGGGCGCTGGAGATTACGGCCGCAGGTGGACACAATCTTC
TTTTGATCGGCCCGCCTGGTACGGGTAAAACGATGCTGGCGAGCAGGCTGAGTGGCTTGCTGCCACCGCTCAATAATCAT
GAAGCGCTGGAGAGTGCCGCCATTTATAGCCTTATCAGTTCTACCTCCTTGCAAAAACAGTGGCGCCGTCGCCCTTTTCG
TTCACCTCATCATAGCGCTTCACTGACGGCGATGGTCGGCGGTGGGTCTATTCCCGGGCCGGGTGAAATCTCACTGGCGC
ACAATGGCATTCTGTTTCTCGACGAGCTGCCTGAGTTTGAGCGCCGCGTCCTGGATGCCCTGAGAGAACCCATTGAATCT
GGAGAGATACACATCTCACGCACGCGAGCCAAAATAAGCTATCCCGCGCAGTTTCAGCTGGTCGCTGCGATGAATCCCAG
CCCTACGGGTCATTATCAGGGTAATCATAACCGCTGTACGCCAGAGCAGACGTTGCGCTATCTGGGAAAGTTATCCGGCC
CGTTTCTCGACCGTTTCGATTTATCCCTCGAAATCCCCCTTCCCCCGCCGGGCCTGCTCAGGCAAAAAGGCATCACGGGT
GAAAGCTCAGCAGATGTACGCGAGCGGGTCATTGCTGCCCAGACGCGACAATATGCCCGTCAGAACAGGCTGAATGCGCG
GCTGGATAATGCCGGGATCCGGCAGTTTTGTTCTCTTAACATGGAGGATGCGGTTTGGCTGGAGGAAACCTTGACGCGTT
TTGGTCTTTCTATACGCGCGTGGCAGCGTTTGCTGAAAGTGGCCAGAACCATTGCTGACGTGGAGGGTTGTAGTGACATT
CAGAGGAAACACTTGCAGGAGGCGCTGAGCTACCGCGCTATCGATCGTTTGCTGCTCCATCTGCAGAAGTTGCTGGCGTA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J0PD61

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.153

100

0.585
  comM Vibrio cholerae strain A1552

58.648

99.407

0.583
  comM Glaesserella parasuis strain SC1401

58.185

100

0.583
  comM Vibrio campbellii strain DS40M4

58.333

99.605

0.581
  comM Legionella pneumophila str. Paris

50.101

98.221

0.492
  comM Legionella pneumophila strain ERS1305867

50.101

98.221

0.492
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.181

100

0.443

Multiple sequence alignment