Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KHN79_RS12510 Genome accession   NZ_HG992749
Coordinates   2741434..2742027 (-) Length   197 a.a.
NCBI ID   WP_182011508.1    Uniprot ID   -
Organism   Vibrio sp. B1FLJ16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2736434..2747027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN79_RS12490 (ACOMICROBIO_LOCUS1816) aroB 2737325..2738425 (-) 1101 WP_182011504.1 3-dehydroquinate synthase -
  KHN79_RS12495 (ACOMICROBIO_LOCUS1817) aroK 2738457..2738975 (-) 519 WP_182011505.1 shikimate kinase AroK -
  KHN79_RS12500 (ACOMICROBIO_LOCUS1818) pilQ 2739164..2740897 (-) 1734 WP_182011506.1 type IV pilus secretin PilQ family protein Machinery gene
  KHN79_RS12505 (ACOMICROBIO_LOCUS1819) pilP 2740929..2741444 (-) 516 WP_182011507.1 pilus assembly protein PilP Machinery gene
  KHN79_RS12510 (ACOMICROBIO_LOCUS1820) pilO 2741434..2742027 (-) 594 WP_182011508.1 type 4a pilus biogenesis protein PilO Machinery gene
  KHN79_RS12515 (ACOMICROBIO_LOCUS1821) pilN 2742020..2742595 (-) 576 WP_182011509.1 PilN domain-containing protein Machinery gene
  KHN79_RS12520 (ACOMICROBIO_LOCUS1822) pilM 2742579..2743595 (-) 1017 WP_182011510.1 type IV pilus assembly protein PilM Machinery gene
  KHN79_RS12525 (ACOMICROBIO_LOCUS1823) - 2743738..2746305 (+) 2568 WP_182011511.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23019.50 Da        Isoelectric Point: 4.8225

>NTDB_id=1112450 KHN79_RS12510 WP_182011508.1 2741434..2742027(-) (pilO) [Vibrio sp. B1FLJ16]
MVNWQDLEMDEIAEWPLIPQLITIGLIVLLLQGVGYWFYLTPKEERLNSLIQQEETLKVTLRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSESFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=1112450 KHN79_RS12510 WP_182011508.1 2741434..2742027(-) (pilO) [Vibrio sp. B1FLJ16]
ATGGTTAATTGGCAAGATCTGGAAATGGATGAGATTGCTGAATGGCCTTTAATACCTCAGTTGATCACCATTGGGTTGAT
TGTTCTCCTTTTGCAGGGCGTTGGTTACTGGTTTTATCTCACTCCTAAGGAAGAGCGTCTGAATAGTCTCATCCAGCAGG
AAGAGACGCTGAAAGTCACATTGCGAATTAAAGCTAATAAGGTTGCTGCACTGCCGCAACTTCAGTCCCAGTTAGATGAG
TTAACAACCCGCTACGAGTTACTGCTGCAGCAATTGCCAGCACAAAAAGAACTCGCGACTATGCTGGCATCAGTGAACGA
GTTGGGTATCGACAACAAACTGACCTTTACCCGTATCGACTGGGGTGAAAAGCAGTCTGAAAGCTTTTTGTACCGACTAC
CACTTAACATCGAGTTAACCGGGGATTTTCATGACATCGGTCGTTTCTCACAGGCGATTGCGACTTTACCACGCATCATC
ACGTTCAAAGATGTGACCTGGCAGCGCGTCAGTCAGGAGAGTGAAACGCTGCACTTTCGTGTGAGAGCGAACACCTACCA
GTTCAAACCAGAGGAAAAGAAAGATGAAAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

89.474

96.447

0.863
  pilO Vibrio cholerae strain A1552

72.081

100

0.721

Multiple sequence alignment