Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KHN79_RS12520 Genome accession   NZ_HG992749
Coordinates   2742579..2743595 (-) Length   338 a.a.
NCBI ID   WP_182011510.1    Uniprot ID   -
Organism   Vibrio sp. B1FLJ16     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2737579..2748595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN79_RS12495 (ACOMICROBIO_LOCUS1817) aroK 2738457..2738975 (-) 519 WP_182011505.1 shikimate kinase AroK -
  KHN79_RS12500 (ACOMICROBIO_LOCUS1818) pilQ 2739164..2740897 (-) 1734 WP_182011506.1 type IV pilus secretin PilQ family protein Machinery gene
  KHN79_RS12505 (ACOMICROBIO_LOCUS1819) pilP 2740929..2741444 (-) 516 WP_182011507.1 pilus assembly protein PilP Machinery gene
  KHN79_RS12510 (ACOMICROBIO_LOCUS1820) pilO 2741434..2742027 (-) 594 WP_182011508.1 type 4a pilus biogenesis protein PilO Machinery gene
  KHN79_RS12515 (ACOMICROBIO_LOCUS1821) pilN 2742020..2742595 (-) 576 WP_182011509.1 PilN domain-containing protein Machinery gene
  KHN79_RS12520 (ACOMICROBIO_LOCUS1822) pilM 2742579..2743595 (-) 1017 WP_182011510.1 type IV pilus assembly protein PilM Machinery gene
  KHN79_RS12525 (ACOMICROBIO_LOCUS1823) - 2743738..2746305 (+) 2568 WP_182011511.1 PBP1A family penicillin-binding protein -
  KHN79_RS12530 (ACOMICROBIO_LOCUS1824) oxyR 2746374..2747282 (-) 909 WP_182011512.1 DNA-binding transcriptional regulator OxyR -
  KHN79_RS12535 (ACOMICROBIO_LOCUS1825) - 2747440..2748168 (+) 729 WP_182011513.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37586.69 Da        Isoelectric Point: 6.8887

>NTDB_id=1112452 KHN79_RS12520 WP_182011510.1 2742579..2743595(-) (pilM) [Vibrio sp. B1FLJ16]
MDKLIVTGIDIGHNSLKAVVLKPNGDKYALLGYKEILLKDGIVAENNTINHQEIVNTLKQLKKELPFGARKVAISVPDNA
VISKKLNIEQNVEENEVEFAVVQAFSHQSPFPVEELSLDFVRLNTEEGQRGVASYQVFATRKDVVESRVEALNKSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYGLLDVGSHSSSFTMFTEQGELFHKEFACGTSSLPRTSQEESLTEDAHSQTEQFN
RQMVERVKRQIQLYTSINGSQNIKGIWLSGGGAATPMLAEELSHQLALECELLNPLGLFEMNVSKRKRRAADWQHFSTAA
GLAVRGIQWLGGQRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1112452 KHN79_RS12520 WP_182011510.1 2742579..2743595(-) (pilM) [Vibrio sp. B1FLJ16]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCACAACAGCCTCAAAGCCGTAGTACTTAAACCCAATGGCGATAA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGACGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTCAAGCAGCTGAAAAAAGAGCTGCCTTTTGGGGCTCGGAAAGTCGCAATTTCTGTTCCTGACAATGCG
GTTATCAGCAAAAAGCTCAACATTGAACAAAACGTCGAAGAAAACGAGGTTGAGTTTGCGGTTGTGCAGGCTTTTTCTCA
CCAATCCCCTTTCCCGGTTGAAGAACTGAGTCTGGACTTTGTCCGTCTTAACACTGAAGAAGGGCAGAGAGGAGTAGCCA
GCTATCAGGTGTTTGCTACGCGTAAAGATGTTGTAGAAAGCCGGGTTGAAGCATTAAACAAATCTGGTCTTAAACCTGTT
TTGGTTGACGTACATTCACAAAGTCTCGGGCATATCTGGAAACTGGCAGCAGAACGTTTTCCGGATAAGAACAAGTACGG
GTTGCTCGATGTTGGCTCACATTCTAGCTCGTTCACGATGTTCACAGAGCAAGGGGAGCTTTTCCATAAAGAGTTTGCCT
GCGGAACATCTTCTCTTCCTCGGACTTCTCAAGAGGAGTCTCTGACCGAAGACGCGCACAGCCAGACGGAACAATTTAAC
CGACAGATGGTTGAACGGGTTAAGCGTCAGATTCAGTTGTATACCTCTATCAATGGTTCACAAAATATCAAAGGTATTTG
GTTATCGGGCGGTGGAGCCGCAACCCCAATGCTTGCAGAAGAACTATCGCATCAGCTGGCACTGGAGTGCGAGTTACTAA
ACCCGCTAGGTCTGTTTGAGATGAATGTGTCTAAACGCAAGCGCAGGGCAGCTGACTGGCAGCACTTTTCTACCGCTGCG
GGGCTGGCAGTCAGAGGTATTCAATGGTTAGGAGGTCAACGTGCTGTATCGCATTAA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.249

100

0.822
  pilM Vibrio cholerae strain A1552

51.045

99.112

0.506

Multiple sequence alignment