Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACMCNJ_RS05160 Genome accession   NZ_AP038788
Coordinates   1103579..1104016 (-) Length   145 a.a.
NCBI ID   WP_226691633.1    Uniprot ID   -
Organism   Rodentibacter sp. THUN1656     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1098579..1109016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMCNJ_RS05130 (THUN1656_09690) - 1099186..1099467 (-) 282 WP_226691638.1 GNAT family N-acetyltransferase -
  ACMCNJ_RS05135 (THUN1656_09700) yacG 1099464..1099670 (-) 207 WP_226691637.1 DNA gyrase inhibitor YacG -
  ACMCNJ_RS05140 (THUN1656_09710) coaE 1099663..1100283 (-) 621 WP_226692923.1 dephospho-CoA kinase -
  ACMCNJ_RS05145 (THUN1656_09720) pilD 1100283..1100984 (-) 702 WP_226691636.1 prepilin peptidase Machinery gene
  ACMCNJ_RS05150 (THUN1656_09730) pilC 1100981..1102201 (-) 1221 WP_226691635.1 type II secretion system F family protein Machinery gene
  ACMCNJ_RS05155 (THUN1656_09740) pilB 1102203..1103582 (-) 1380 WP_226691634.1 GspE/PulE family protein Machinery gene
  ACMCNJ_RS05160 (THUN1656_09750) pilA 1103579..1104016 (-) 438 WP_226691633.1 prepilin peptidase-dependent pilin Machinery gene
  ACMCNJ_RS05165 (THUN1656_09760) ampD 1104132..1104695 (+) 564 WP_226691632.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMCNJ_RS05170 (THUN1656_09770) rppH 1105212..1105796 (+) 585 WP_226691631.1 RNA pyrophosphohydrolase -
  ACMCNJ_RS05175 (THUN1656_09780) lgt 1105928..1106728 (+) 801 WP_374707287.1 prolipoprotein diacylglyceryl transferase -
  ACMCNJ_RS05180 (THUN1656_09790) - 1106852..1107703 (+) 852 WP_226691630.1 thymidylate synthase -
  ACMCNJ_RS05185 (THUN1656_09800) tadA 1107715..1108221 (+) 507 WP_255618266.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15020.27 Da        Isoelectric Point: 8.4769

>NTDB_id=111217 ACMCNJ_RS05160 WP_226691633.1 1103579..1104016(-) (pilA) [Rodentibacter sp. THUN1656]
MKLTSQRTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAMSELLQAATPFKSDVELCVYSTGATANCSGGSNGIA
ADITSPKGYVKGISTNAGVITVVGNGALDGVQYTLTATGDHSRGISWSTTCNGDTSLFPAGFCTK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=111217 ACMCNJ_RS05160 WP_226691633.1 1103579..1104016(-) (pilA) [Rodentibacter sp. THUN1656]
ATGAAACTCACCTCTCAACGCACATTGAAAAAAGGATTTACACTAATCGAATTAATGATCGTGATAGCCATCATCGCGAT
TCTCGCGACAATTGCAATTCCCTCTTATCAGAATTATACAAAAAAGGCGGCGATGTCTGAATTGTTACAAGCCGCAACCC
CTTTTAAATCCGACGTGGAACTCTGCGTTTATAGCACAGGAGCAACCGCAAATTGCAGCGGCGGTTCAAACGGTATCGCT
GCCGATATTACCTCACCAAAAGGCTATGTGAAAGGTATTAGTACTAACGCCGGCGTTATTACTGTTGTCGGCAACGGCGC
TTTAGACGGTGTCCAATATACATTAACCGCCACCGGTGATCATTCTAGAGGAATAAGCTGGTCTACAACCTGTAACGGTG
ATACCTCCTTATTTCCGGCAGGATTCTGTACAAAATGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

72.222

99.31

0.717
  pilA Haemophilus influenzae Rd KW20

69.444

99.31

0.69
  pilA Glaesserella parasuis strain SC1401

57.746

97.931

0.566
  pilA Vibrio campbellii strain DS40M4

38.71

100

0.414

Multiple sequence alignment