Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGHP2_RS18875 Genome accession   NZ_AP035784
Coordinates   4253156..4254892 (+) Length   578 a.a.
NCBI ID   WP_368352429.1    Uniprot ID   -
Organism   Acidovorax sacchari strain HC-21     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4248156..4259892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHP2_RS18845 (AVXHC21_37510) proB 4248610..4249749 (-) 1140 WP_368352426.1 glutamate 5-kinase -
  ACGHP2_RS18850 (AVXHC21_37520) cgtA 4249828..4250901 (-) 1074 WP_368352427.1 Obg family GTPase CgtA -
  ACGHP2_RS18855 (AVXHC21_37530) rpmA 4250998..4251255 (-) 258 WP_011796716.1 50S ribosomal protein L27 -
  ACGHP2_RS18860 (AVXHC21_37540) rplU 4251268..4251579 (-) 312 WP_011796717.1 50S ribosomal protein L21 -
  ACGHP2_RS18865 (AVXHC21_37550) - 4251765..4252745 (+) 981 WP_368352428.1 polyprenyl synthetase family protein -
  ACGHP2_RS18875 (AVXHC21_37560) pilB 4253156..4254892 (+) 1737 WP_368352429.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHP2_RS18880 (AVXHC21_37570) pilC 4254924..4256141 (+) 1218 WP_415266442.1 type II secretion system F family protein Machinery gene
  ACGHP2_RS18885 (AVXHC21_37580) - 4256141..4257073 (+) 933 WP_415266443.1 prepilin peptidase -
  ACGHP2_RS18890 (AVXHC21_37590) coaE 4257070..4257696 (+) 627 WP_415266444.1 dephospho-CoA kinase -
  ACGHP2_RS18895 (AVXHC21_37600) zapD 4257761..4258516 (+) 756 WP_368352433.1 cell division protein ZapD -
  ACGHP2_RS18900 (AVXHC21_37610) - 4258543..4258800 (+) 258 WP_368352434.1 DNA gyrase inhibitor YacG -
  ACGHP2_RS18905 (AVXHC21_37620) - 4258826..4259281 (-) 456 WP_368352435.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63294.56 Da        Isoelectric Point: 5.3406

>NTDB_id=110825 ACGHP2_RS18875 WP_368352429.1 4253156..4254892(+) (pilB) [Acidovorax sacchari strain HC-21]
MADADYALKDASSIALPGLGRALISAGQLSQKVAEDIYRKSQVNRSSFIAELTGSGSVSPINLAHTVSSVFGAPLLDIEA
IDPLRLPRDVLDPKICQAYRVIVLSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLVRLVDATTKSTTETM
ESMVSGGDFEFDDLPSQESTEAQDTAATEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPP
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKE
RLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPACKAPADIP
HETLLEAGYKEEDIDGSWVTYRPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAEQAKAEGVRSLRESGLH
KARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=110825 ACGHP2_RS18875 WP_368352429.1 4253156..4254892(+) (pilB) [Acidovorax sacchari strain HC-21]
ATGGCTGACGCCGATTACGCCCTCAAGGATGCTTCCAGTATCGCCCTGCCCGGCCTGGGGCGGGCATTGATCTCGGCCGG
GCAACTGAGCCAGAAGGTGGCGGAGGACATCTACCGGAAGTCCCAGGTCAACCGGAGCAGTTTCATCGCGGAGCTGACCG
GATCGGGTTCGGTTTCCCCGATCAACCTGGCGCATACCGTGTCTTCGGTGTTCGGCGCCCCCCTGCTCGACATCGAGGCC
ATCGATCCGCTGCGCCTGCCGCGCGACGTGCTGGATCCGAAGATCTGCCAGGCGTACCGGGTCATCGTGCTGAGCAAGCG
CAACAACCGGCTCATCGTCGCGACGGCGGACCCGACCGACCAGGAGGCGGCGGAGAAGATCAAGTTCACCACGCAGATGG
GCGTGGACTGGATCATCGCCGAGTACGACAAGCTGGTGCGGCTGGTCGATGCGACCACCAAGTCCACGACCGAGACCATG
GAAAGCATGGTCTCCGGGGGCGATTTCGAGTTCGACGACCTGCCGTCGCAGGAGAGTACCGAAGCCCAGGACACGGCCGC
GACGGAGGTCGAAGACGCCCCCATCGTCAAGTTCCTGCACAAGATGCTGCTGGACGCGTTCAACATGCGCGCGTCCGACC
TGCACTTCGAGCCGTACGAGCACCAGTACCGGGTCCGCTTCCGCATCGACGGCGAGCTGCGCGAGATCGCGTCGCCGCCC
ATCGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCCCGCCTCGACATCTCGGAAAAGCGCGTGCCCCAGGA
CGGGCGCATGAAGCTCAAGGTGGGGCCGGACCGGGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGA
AGATCGTGATCCGTATCCTCGACCCCAGCAGCGCCAAGCTGGGCATCGATGCGCTCGGCTACGAGCCCGAGGAGAAGGAG
CGCCTGCTCCATGCCATCGGCCGGCCCTACGGCATGATCCTGGTGACGGGGCCGACGGGCTCGGGCAAGACGGTGTCTCT
ATACACCTGCCTGAACCTGCTGAACAAGCCGGGGGTCAACATCGCGACGGCGGAAGACCCGTCGGAAATCAACCTGCCCG
GCGTCAACCAGGTCAACGTGAACGAAAAGGCCGGGCTGACCTTCGCCACGGCGCTCAAGTCGTTCCTGCGCCAGGATCCC
GACATCATCATGGTGGGGGAAATCCGCGACCTGGAAACCGCGGACATCTCGATCAAGGCGGCCCAGACGGGCCACCTGGT
GCTGTCCACGCTGCACACCAACGATGCTCCCACGACGCTGACGCGGATGCGGAACATGGGCATCGCGCCCTTCAACATCG
CGTCGAGCGTGATCCTGATCACGGCGCAGCGCCTGGCCCGCCGGCTGTGCCCTGCGTGCAAGGCGCCCGCGGACATCCCC
CACGAGACGCTGCTCGAGGCGGGCTACAAGGAAGAGGACATCGACGGCTCCTGGGTGACCTATCGCCCGGTGGGCTGCCC
GGCCTGCAACAACGGCTACAAGGGCCGCGTCGGCATCTACCAGGTCATGCCCATCTCGGAAGAGATCCAGCGCATCATCC
TGCGCGACGGCAGCGCGCTGGAGATCGCGGAGCAGGCGAAGGCCGAAGGCGTGCGCTCGCTGCGCGAATCCGGACTCCAC
AAGGCGCGGCTCGGGCTGACCTCGCTCGAAGAGGTGCTGGCCGTGACGAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.833

98.443

0.54
  pilB Acinetobacter baumannii D1279779

53.054

99.135

0.526
  pilF Neisseria gonorrhoeae MS11

52.837

97.578

0.516
  pilB Legionella pneumophila strain ERS1305867

49.468

97.578

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.369

92.907

0.431
  pilB Vibrio campbellii strain DS40M4

44.385

97.059

0.431
  pilB Vibrio cholerae strain A1552

47.238

90.83

0.429
  pilF Thermus thermophilus HB27

39.145

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.373

99.308

0.391

Multiple sequence alignment