Detailed information NTG0110621

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACAM27_RS16830
• Genome accession: NZ_AP031605
• Coordinates: 3708840..3709904 (-)
• Length: 354 a.a.
• NCBI ID: WP_011784345.1
• Uniprot ID: A0A368USH3
• Organism: Marinobacter sp. RI1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3703840..3714904

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACAM27_RS16805 (MspRI1_33310)	aroK	3704278..3704868 (-)	591	WP_023010980.1	shikimate kinase AroK	-
ACAM27_RS16810 (MspRI1_33320)	pilQ	3705004..3707106 (-)	2103	WP_369612339.1	type IV pilus secretin PilQ	Machinery gene
ACAM27_RS16815 (MspRI1_33330)	-	3707121..3707663 (-)	543	WP_153741467.1	pilus assembly protein PilP	-
ACAM27_RS16820 (MspRI1_33340)	-	3707666..3708277 (-)	612	WP_224851272.1	type 4a pilus biogenesis protein PilO	-
ACAM27_RS16825 (MspRI1_33350)	-	3708277..3708840 (-)	564	WP_011784346.1	PilN domain-containing protein	-
ACAM27_RS16830 (MspRI1_33360)	pilM	3708840..3709904 (-)	1065	WP_011784345.1	pilus assembly protein PilM	Machinery gene
ACAM27_RS16835 (MspRI1_33370)	-	3710142..3712682 (+)	2541	WP_374730293.1	penicillin-binding protein 1A	-
ACAM27_RS16840 (MspRI1_33380)	-	3712769..3714034 (-)	1266	WP_222534799.1	malic enzyme-like NAD(P)-binding protein	-
ACAM27_RS16845 (MspRI1_33390)	rpmE	3714248..3714469 (-)	222	WP_014420566.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37993.03 Da
• Isoelectric Point: 4.1824

>NTDB_id=110621 ACAM27_RS16830 WP_011784345.1 3708840..3709904(-) (pilM) [Marinobacter sp. RI1]
MFGLPGKKSSAVLGVDVSSSSVKLLELSKHGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTSVKQVAV
AVSGSAVITKVIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDQVDVLLAACRKENVDIREDALEI
AGLTTKIVDVEAYALERAYSLIEPQLDSQGEELVVAIVDIGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYNEEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FANMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=110621 ACAM27_RS16830 WP_011784345.1 3708840..3709904(-) (pilM) [Marinobacter sp. RI1]
GTGTTCGGATTACCAGGAAAAAAATCCAGTGCCGTCCTTGGAGTGGATGTAAGCTCCAGTTCGGTCAAGCTGCTAGAGCT
GTCAAAACATGGCGATCGATATAAGGTCGAGAGCTATGCGGTAGAGCCGTTGCCGGCCAATGCAGTTGTTGAAAAAAACA
TCACAGACGTCGAAGCGGTTGGGGAAGTGCTCAAGCGTGTCGCGTCCAAGTCACGCACCAGTGTGAAACAGGTGGCTGTT
GCTGTTTCCGGCTCGGCGGTGATCACCAAGGTTATCCAGATGGATGGTGGTCTCAACGAGTTTGAAATGGAAGACCAGAT
CGCGCTGGAAGCTGATCAGTACATTCCTTACCCCCTTGATGAAGTGGCGATCGATTTCGAAGTCCAGGGCCCGTCCGAAT
CCAACCCGGATCAGGTGGATGTGCTGCTGGCGGCCTGCCGTAAGGAAAACGTCGATATCCGCGAAGACGCCCTGGAAATT
GCAGGGCTGACAACCAAGATTGTCGATGTGGAAGCCTACGCCCTGGAGCGAGCCTACAGCCTGATTGAGCCTCAGCTTGA
CTCCCAGGGAGAAGAGTTGGTGGTTGCCATCGTTGATATCGGTGCAACCATGACAACTCTTAGTGTGCTGGCAGAGGGTA
AGACGGTATATACCCGTGAGCAGATCTTTGGCGGAAAACAGCTGACTGAAGAAATTCAGCGTCGTTATGGCCTGTCGCTC
GAAGAGGCTGGTCTGGCCAAGAAACAGGGTGGTTTGCCGGACGACTACAACGAGGAAGTTCTGACTCCGTTCCGGGAAGC
GGTTGTTCAGCAAGTGGCGCGGGCGCTGCAGTTTTTCTTCGGCGCCAGTCAGTACAATGCGGTGGATTACGTAGTGCTTG
CCGGTGGAACAGCGTCGATTCAGGGGCTCACGGAAATGGTGGAAGAGAAAACCGGAACGCCAACCCTGGTGGCCAATCCG
TTTGCCAACATGGCGGTGGGTTCCCGGGTCAACGCCTCTGCGCTCAGCAATGATGCCCCCTCGTTGATGATTGCCTGCGG
TCTGGCAATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A368USH3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.672	100	0.537
comM	Acinetobacter nosocomialis M2	53.672	100	0.537
comM	Acinetobacter baylyi ADP1	51.695	100	0.517
pilM	Legionella pneumophila strain ERS1305867	45.198	100	0.452