Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACNVED_RS03690 Genome accession   NZ_CP183303
Coordinates   829558..830157 (+) Length   199 a.a.
NCBI ID   WP_419421141.1    Uniprot ID   -
Organism   Legionella sp. D16C41     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824558..835157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNVED_RS03675 (ACNVED_03675) - 826692..827687 (-) 996 WP_419420410.1 hypothetical protein -
  ACNVED_RS03680 (ACNVED_03680) pilM 827940..828998 (+) 1059 WP_419420411.1 type IV pilus assembly protein PilM Machinery gene
  ACNVED_RS03685 (ACNVED_03685) pilN 829004..829558 (+) 555 WP_419420412.1 PilN domain-containing protein Machinery gene
  ACNVED_RS03690 (ACNVED_03690) pilO 829558..830157 (+) 600 WP_419421141.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACNVED_RS03695 (ACNVED_03695) - 830154..830666 (+) 513 WP_419420413.1 pilus assembly protein PilP -
  ACNVED_RS03700 (ACNVED_03700) pilQ 830677..832815 (+) 2139 WP_419420414.1 type IV pilus secretin PilQ Machinery gene
  ACNVED_RS03705 (ACNVED_03705) aroK 833136..833663 (+) 528 WP_419420415.1 shikimate kinase AroK -
  ACNVED_RS03710 (ACNVED_03710) aroB 833650..834756 (+) 1107 WP_419420416.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 23072.18 Da        Isoelectric Point: 8.3117

>NTDB_id=1105171 ACNVED_RS03690 WP_419421141.1 829558..830157(+) (pilO) [Legionella sp. D16C41]
MNNLNLSELTLENVGQWPPIIKYLIAASLIAIIIVFGYLLIIKQNLESRDSYIAQEKTLREEFERKQQLAANLQTYKMQL
QIMNERFGTMLKQLPAENEMPGLLEDISKTGIASGLAFDLFAPQTEVKHDFYIELPIKLTVIGNYHQLAVFISRIIQMSR
IVTLHDFEINPQMDEKLKKPIKDLLIMQLTAKIYRYKTK

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1105171 ACNVED_RS03690 WP_419421141.1 829558..830157(+) (pilO) [Legionella sp. D16C41]
ATGAATAATTTAAATTTAAGTGAATTAACATTAGAAAATGTTGGCCAATGGCCACCTATAATAAAATACTTAATAGCTGC
ATCGTTAATAGCGATTATCATCGTCTTTGGTTATTTATTAATAATAAAACAAAATCTTGAATCACGTGATAGTTATATAG
CGCAGGAAAAAACATTGCGTGAAGAATTTGAAAGAAAACAGCAATTAGCAGCTAATTTACAAACCTATAAAATGCAGTTA
CAAATCATGAATGAGCGATTTGGCACTATGCTAAAACAGCTTCCAGCAGAAAATGAAATGCCAGGTTTATTAGAAGATAT
CTCCAAAACGGGTATAGCTAGTGGCTTAGCTTTTGATCTATTTGCTCCCCAAACTGAGGTAAAGCACGATTTCTATATTG
AGCTTCCAATCAAACTTACTGTAATAGGCAACTATCATCAATTAGCTGTGTTTATTAGTCGCATTATTCAAATGAGTCGT
ATTGTTACTTTACATGATTTTGAGATAAATCCACAGATGGATGAAAAATTAAAAAAACCTATAAAAGATTTATTAATAAT
GCAATTAACAGCAAAAATTTATAGATATAAAACCAAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(54-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

59.799

100

0.598
  comO Acinetobacter baylyi ADP1

41.27

94.975

0.392
  pilO Acinetobacter baumannii D1279779

38.947

95.477

0.372

Multiple sequence alignment