Detailed information NTG1104388

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACNM63_RS12295
• Genome accession: NZ_CP183191
• Coordinates: 2545329..2545943 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain QC02
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2540329..2550943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNM63_RS12265 (ACNM63_12265)	rpsT	2540632..2540898 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ACNM63_RS12270 (ACNM63_12270)	holA	2540861..2541956 (-)	1096	Protein_2369	DNA polymerase III subunit delta	-
ACNM63_RS12275 (ACNM63_12275)	-	2541996..2542147 (-)	152	Protein_2370	hypothetical protein	-
ACNM63_RS12280 (ACNM63_12280)	-	2542188..2542322 (+)	135	WP_003152870.1	YqzM family protein	-
ACNM63_RS12285 (ACNM63_12285)	comEC	2542341..2544692 (-)	2352	WP_419246904.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACNM63_RS12290 (ACNM63_12290)	-	2544693..2545262 (-)	570	WP_003152868.1	ComE operon protein 2	-
ACNM63_RS12295 (ACNM63_12295)	comEA	2545329..2545943 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACNM63_RS12300 (ACNM63_12300)	comER	2546002..2546823 (+)	822	WP_014418420.1	late competence protein ComER	-
ACNM63_RS12305 (ACNM63_12305)	-	2546892..2547629 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
ACNM63_RS12310 (ACNM63_12310)	rsfS	2547626..2547982 (-)	357	WP_007408260.1	ribosome silencing factor	-
ACNM63_RS12315 (ACNM63_12315)	yqeK	2548000..2548560 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACNM63_RS12320 (ACNM63_12320)	-	2548550..2549119 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ACNM63_RS12325 (ACNM63_12325)	yhbY	2549130..2549420 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ACNM63_RS12330 (ACNM63_12330)	aroE	2549414..2550256 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=1104388 ACNM63_RS12295 WP_014418419.1 2545329..2545943(-) (comEA) [Bacillus velezensis strain QC02]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1104388 ACNM63_RS12295 WP_014418419.1 2545329..2545943(-) (comEA) [Bacillus velezensis strain QC02]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363